Protective effect of melatonin against multistress condition induced lipid peroxidation via measurement of gastric mucosal lesion and plasma malondialdehyde levels in rats

doi:10.3748/wjg.v12.i46.7527

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Dec 14, 2006 (publication date) through May 30, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 14, 2006; 12(46): 7527-7531
Published online Dec 14, 2006. doi: 10.3748/wjg.v12.i46.7527

Protective effect of melatonin against multistress condition induced lipid peroxidation via measurement of gastric mucosal lesion and plasma malondialdehyde levels in rats

V Kiarostami, L Samini, M Ghazi-Khansari

V Kiarostami, L Samini, Faculty of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, Iran

M Ghazi-Khansari, Department of Pharmacology, School of Medicine, Tehran University of Medical sciences, Tehran, Iran

Author contributions: All authors contributed equally to the work.

Correspondence to: M Ghazi-Khansari, PhD, Associate Professor, Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, PO Box:13145-784, Tehran, Iran. ghazikha@sina.tums.ac.ir

Telephone: +98-21-66402569 Fax: +98-21-66402569

Received: July 22, 2006
Revised: October 15, 2006
Accepted: October 23, 2006
Published online: December 14, 2006

Abstract

AIM: To study the protective effect of a natural antioxidant, melatonin, against multistress condition induced lipid peroxidation via determination of gastric damage and plasma malondialdehyde (MDA) level by high performance liquid chromatography in rats.

METHODS: We compared indomethacin-induced gastric damage and MDA plasma level in three groups of rats: unoperated, bile duct ligated and sham-operated and evaluated the role of the melatonin on gastric damage and plasma MDA level. Indomethacin and melatonin were injected intraperitoneally in doses of 50 mg/kg and 20 mg/kg, respectively. Animals were killed 4 h after indomethacin injection.

RESULTS: Indomethacin induced more severe gastric damage and plasma MDA level in bile duct ligated animals was significantly higher (3.1 ± 0.04 μmol/L) than sham (2.8 ± 0.04 μmol/L) and unoperated animals (1.4 ± 0.08 μmol/L). Pretreatment with melatonin reduced indomethacin-induced gastric damage and plasma MDA level.

CONCLUSION: Considering the results of this study, we suggest that in multistress conditions the intensity of gastric damage and the plasma MDA level are great and melatonin reduces the negative effect of lipid peroxidation and cell damage by oxidative stress in multistress conditions due to its antioxidizing activity.

Keywords: Melatonin, Oxidative stress, Gastric ulcer, Cholestasis, Malondialdehyde, High performance liquid chromatography, Rat