H Pylori
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Sep 28, 2006; 12(36): 5793-5797
Published online Sep 28, 2006. doi: 10.3748/wjg.v12.i36.5793
Human β-defensin-3 induction in H pylori-infected gastric mucosal tissues
K Kawauchi, A Yagihashi, N Tsuji, N Uehara, D Furuya, D Kobayashi, N Watanabe
K Kawauchi, A Yagihashi, N Tsuji, N Uehara, D Furuya, D Kobayashi, N Watanabe, Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan
Author contributions: All authors contributed equally to the work.
Correspondence to: Atsuhito Yagihashi, MD, PhD, Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan. yagihasi@sapmed.ac.jp
Telephone: +81-11-6112111-3640 Fax: +81-11-6227502
Received: March 28, 2006
Revised: June 5, 2006
Accepted: June 14, 2006
Published online: September 28, 2006
Abstract

AIM: To examine human β-defensin-3 (hBD-3) expression in inflamed gastric mucosal tissues or MKN45 gastric cancer cells with or without H pylori infection for better understanding the innate immune response to H pylori.

METHODS: We used reverse transcription-polymerase chain reactions and immunohistochemistry to examine hBD-3 expression in inflamed gastric mucosal tissues or MKN45 gastric cancer cells with or without H pylori. Effects of hBD-3 against H pylori were also evaluated.

RESULTS: The mean mRNA expression of hBD-3 in H pylori-positive specimens was significantly higher than that in H pylori-negative specimens (P = 0.0002, Mann-Whitney). In addition, unlike uninfected samples, 8 of 15 (53.33%) infected mucosal samples expressed hBD-3 protein. H pylori dose-dependently induced mRNA expression of hBD-3 in MKN45 cells, an effect inhibited by adding anti-toll-like receptor (TLR)-4 antibody. HBD-3 protein completely inhibited H pylori growth.

CONCLUSION: Our results suggest that like hBD-2, hBD-3 may be involved in the pathophysiology of H pylori-induced gastritis.

Keywords: Human β-defensin-2, Human β-defensin-3, H pylori, Gastric mucosa