Identification of key genes and pathways in gastric signet ring cell carcinoma based on transcriptome analysis

Figure 1 Summary of transcriptome sequencing results of mRNA expression in gastric carcinoma. A: Circos plot shows the differential genes on the human chromosomes. From the outside and inwards, the first layer of the Circos plot is a chromosome map of the human genome; the black and white bars represent expression, while the third layer of circus plot represents differential genes, red indicates up-regulated genes, and green indicates down-regulated genes; B: Volcano plot of the distributions of mRNAs; C: Numbers of differential genes between gastric signet ring cell carcinoma and non-gastric signet ring cell carcinoma; D: Cluster analysis of functional mRNAs in four cases of gastric carcinoma, and data are presented as a heat map (P < 0.05). GSRCC: Gastric signet ring cell carcinoma.

Figure 2 KEGG and PANTHER pathway analysis of differential genes. A: Top 15 enriched KEGG pathways are presented; B: Top 15 enriched PANTHER pathways are presented.

Figure 3 Protein-protein interaction network of differential genes. Using the STRING online database, 310 genes were selected and used to construct the protein-protein interaction network.

Figure 4 Expression of MAGEA2/3/6, MAGEA4, and REG1B in gastric signet ring cell carcinoma. Data were collected from TCGA and GTEx data using GEPIA (http://gepia.cancer-pku.cn/index.html). A: FPKM of genes in transcriptome sequencing data in 408 gastric carcinoma tissues (T) and 211 normal tissues (N); B: Survival rate calculated by the Kaplan-Meier method in patients separated according to the median expression level of each gene (95%CI); C: Quantitative real-time PCR was used to measure the relative expression levels of the indicate genes in 27 gastric signet ring cell carcinoma samples and 29 non-gastric signet ring cell carcinoma samples. The results were normalized to the endogenous GAPDH RNA control. GSRCC: Gastric signet ring cell carcinoma.