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World J Clin Cases. Jun 26, 2022; 10(18): 5934-5945
Published online Jun 26, 2022. doi: 10.12998/wjcc.v10.i18.5934
Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications
Yue-Ying Huang, Xiao-Yu Zhang, Ping Zhu, Ling Ji
Yue-Ying Huang, Xiao-Yu Zhang, Ping Zhu, School of Medical Laboratory, Weifang Medical University, Weifang 261053, Shandong Province, China
Ling Ji, Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen 518035, Guangdong Province, China
Author contributions: Huang YY contributed to the conception of the study; Zhang XY designed the work; Zhu P contributed to the acquisition of the case; Ji L revised the manuscript critically for important intellectual content; all authors have read the manuscript and gave their final approval of the version to be published.
Supported by the Shenzhen Science and Technology R&D Fund, No. JCYJ20190809095203586.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Ling Ji, Doctor, Chief Technician, Department of Laboratory Medicine, Peking University Shenzhen Hospital, No. 1120 Lianhua Road, Futian District, Shenzhen 518035, Guangdong Province, China. 1120303921@qq.com
Received: November 3, 2021
Peer-review started: November 3, 2021
First decision: December 27, 2021
Revised: January 10, 2022
Accepted: April 24, 2022
Article in press: April 24, 2022
Published online: June 26, 2022
Core Tip

Core Tip: This review mainly discusses and explores the potential clinical applications of the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) technology. The detection technologies for nucleic acids and small molecules of different pathogens based on the CRISPR/Cas system are summarized. The advantages and disadvantages of the CRISPR/Cas technology from the aspects of gene editing, disease treatment, multi-drug resistance, and treatment are enumerated, asserting that CRISPR/Cas system has unlimited potential in clinical applications with certain challenges.