Copyright
©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
Association between homeobox protein transcript antisense intergenic ribonucleic acid genetic polymorphisms and cholangiocarcinoma
Dimitra Ioanna Lampropoulou, Konstantinos Laschos, Gerasimos Aravantinos, Konstantinos Georgiou, Konstantinos Papiris, George Theodoropoulos, Maria Gazouli, Dimitrios Filippou
Dimitra Ioanna Lampropoulou, Konstantinos Laschos, Gerasimos Aravantinos, Medical Oncology, General Oncology Hospital of Kifissia “Agioi Anargiroi”, Athens 14564, Greece
Konstantinos Georgiou, George Theodoropoulos, 1st Department of Propaedeutic Surgery, Hippokration General Hospital of Athens, Athens Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece
Konstantinos Papiris, Endoscopic Surgery Department, Hippokration General Hospital, Athens 11527, Greece
Maria Gazouli, Basic Medical Sciences, Athens Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece
Dimitrios Filippou, Anatomy and Surgical Anatomy, Medical School, National and Kapodistrian University of Athens, Athens 11527, Greece
Author contributions: Lampropoulou DI and Laschos K performed the majority of the writing and were involved in the execution of the experiments; Gazouli M conceived the study, performed the majority of experiments, made critical revisions and writing; Papiris K, Aravantinos G, Georgiou K, Theodoropoulos G participated in the collection of the human samples and provided substantial contributions to the conception and design of the study; Filippou D made critical revisions and provided approval of the final version of the manuscript to be published.
Supported by The Hellenic Society of Medical Oncology, No. 8021/25.09.2020.
Institutional review board statement: The study was reviewed and approved by the Ippokrateion General Hospital Institutional Review Board (8798/12-6-2020).
Informed consent statement: All patients gave informed consent.
Conflict-of-interest statement: No conflict of interest to declare.
Data sharing statement: Not applicable.
STROBE statement: The authors have read the STROBE Statement-checklist of items, and the manuscript was prepared and revised according to the STROBE Statement-checklist of items.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
http://creativecommons.org/Licenses/by-nc/4.0/ Corresponding author: Dimitrios Filippou, MD, PhD, Assistant Professor, Anatomy and Surgical Anatomy, Medical School, National and Kapodistrian University of Athens, M Asias 75 Gousi, Athens 11527, Greece. d_filippou@hotmail.com
Received: December 16, 2020
Peer-review started: December 16, 2020
First decision: December 31, 2020
Revised: January 4, 2021
Accepted: February 12, 2021
Article in press: February 12, 2021
Published online: March 16, 2021
ARTICLE HIGHLIGHTS
Research background
The role of existing tumor biomarkers for cholangiocarcinoma (CCA) diagnosis, remains controversial due to their low sensitivity and specificity. Increasing evidence has implicated long non-coding ribonucleic acid (lncRNA) polymorphisms with cancer susceptibility in a variety of tumor types.
Research motivation
The association between lncRNA homeobox protein transcript antisense intergenic RNA (HOTAIR) polymorphisms and CCA risk has not been reported yet.
Research objectives
To investigate the role of lncRNA HOTAIR polymorphisms in CCA susceptibility.
Research methods
Case-control study on lncRNA HOTAIR genotypes.
Research results
HOTAIR rs4759314 AG and GG genotypes were associated with a significantly increased CCA risk [P = 0.004, odds ratio: 3.13; 95% confidence interval: 1.65-5.91 and P = 0.005, odds ratio: 12.31; 95% confidence interval: 1.48-101.87, respectively]. However, no significant associations of HOTAIR rs920778, and rs7958904 were detected. Similarly, we found no significant associations between rs4759314 AA genotype and CCA susceptibility.
Research conclusions
HOTAIR rs4759314 AG and GG genotypes may be implicated with CCA development and may serve as a potential diagnostic biomarker.
Research perspectives
To further investigate the biological role of HOTAIR on CCA pathogenesis.
