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World J Methodol. Sep 26, 2013; 3(3): 27-38
Published online Sep 26, 2013. doi: 10.5662/wjm.v3.i3.27
Published online Sep 26, 2013. doi: 10.5662/wjm.v3.i3.27
Bifunctional staining for ex vivo determination of area at risk in rabbits with reperfused myocardial infarction
Yuanbo Feng, Feng Chen, Jie Yu, Marlein Miranda Cona, Yicheng Ni, Theragnostic Laboratory, Department of Imaging and Pathology, Biomedical Sciences Group, 3000 KU Leuven, Belgium
Zhan-Long Ma, Departments of Radiology, the First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Yi Xie, Departments of Electronics and Information System (ELIS), Ghent University, Sint-Pietersnieuwstraat 25, 9000 Gent, Belgium
Yue Li, Lab of Translational Medicine, Jiangsu Academy of Traditional Chinese Medicine, nanjing 210028, Jiangsu Province, China
Yicheng Ni, Radiology Section, University Hospitals, 3000 Leuven, Belgium
Author contributions: All authors made a substantial contribution to the conception and design of the manuscript, drafting the article or revising it.
Supported by The awarded grants of the KU Leuven Molecular Small Animal Imaging Center MoSAIC (KUL EF/05/08); the Center of Excellence in vivo Molecular Imaging Research (IMIR) of KU Leuven; a EU Project Asia-Link CfP 2006-Europe Aid/123738/C/ACT/Multi-Proposal, No. 128-498/111; and Jiangsu Province Natural Science Foundation, China, No. BK2010594
Correspondence to: Yicheng Ni, MD, PhD, Professor, Radiology Section, University Hospitals, KU Leuven Herestraat 49, 3000 Leuven, Belgium. yicheng.ni@med.kuleuven.be
Telephone: +32-16-330165 Fax: +32-16-343765
Received: July 12, 2013
Revised: July 16, 2013
Accepted: August 16, 2013
Published online: September 26, 2013
Revised: July 16, 2013
Accepted: August 16, 2013
Published online: September 26, 2013
Core Tip
Core tip: For developing therapeutic procedures/drugs aimed at modulating infarct size after coronary artery disease, it is important not only to measure myocardial infarction but also to know the extent of area at risk (AAR). However, determination of the AAR both in vivo and ex vivo can be challenging, and controversial. In this experiment we sought to develop a reliable method to accurately localize the culprit coronary occlusion for postmortem verification after in vivo cardiac magnetic resonance imaging and to establish a new bifunctional staining as a standard reference for ex vivo area at risk identification, which may substantially contribute to translational cardiac imaging research.