Basic Study
Copyright ©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Transl Med. Dec 12, 2015; 4(3): 78-87
Published online Dec 12, 2015. doi: 10.5528/wjtm.v4.i3.78
Role of nestin in glioma invasion
Alex Lin, Luigi Marchionni, Jeffrey Sosnowski, David Berman, Charles G Eberhart, Eli E Bar
Alex Lin, David Berman, Charles G Eberhart, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States
Luigi Marchionni, David Berman, Charles G Eberhart, Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States
Jeffrey Sosnowski, Department of Orthopedic Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States
Charles G Eberhart, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States
Eli E Bar, Department of Neurological Surgery, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, United States
Eli E Bar, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, Cleveland, OH 44106, United States
Author contributions: Lin A and Bar EE performed the majority of the experiments; Sosnowski J performed immunohistochemical analyses; Marchionni L performed all statistical analyses; Berman D provided shRNA constructs; Eberhart CG and Bar EE analyzed all the data; Bar EE wrote the paper.
Supported by Brain Tumor Funders Collaborative.
Institutional review board statement: This study was reviewed and approved by the Johns Hopkins University Institutional Review Board (IRB).
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Johns Hopkins Institutional Animal Care and Use Committee (IACUC) (Protocol NO. MO13M434).
Conflict-of-interest statement: The authors declare that there are no conflicts of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Eli E Bar, PhD, Assistant Professor, Department of Neurological Surgery, School of Medicine, Case Western Reserve University, Robbins Building Room E750A, 2210 Circle Drive, Cleveland, OH 44106, United States. eli.bar@case.edu
Telephone: +1-216-3680933 Fax: +1-216-3681144
Received: July 15, 2015
Peer-review started: July 29, 2015
First decision: September 21, 2015
Revised: November 13, 2015
Accepted: December 1, 2015
Article in press: December 2, 2015
Published online: December 12, 2015
Core Tip

Core tip: Despite its common use as a marker of poorly differentiated stem and progenitor cells, the functional role of nestin in normal and neoplastic cells is poorly understood. Here we show that in gliomas, there is a significant positive correlation between nestin protein expression and increasing pathological grade. However, when nestin expression was inhibited, we found no significant effects on cell growth, expression of stem-cell markers, and the ability to initiate intracranial xenografts. Our data suggest that the functional role of nestin is limited, even though the migratory potential of some glioblastoma neurospheres is reduced by nestin knockdown.