Reference gene panel for urinary exosome-based molecular diagnostics in patients with kidney disease

Table 1 Number of subjects enrolled for microarray profiling

Groups	Kidney conditions	Group	Urine samples
Group 1	T2DM patients without kidney disease	Diabetic control	13
Group 2	T2DM patients with kidney disease	Diabetic nephropathy	5
		Membranous nephropathy	5
		IgA nephropathy	2
Group 3	Healthy subjects without T2DM or kidney disease	Healthy control	6

T2DM: Type 2 diabetes mellitus.

Table 2 Demographic and anthropometric details of subjects enrolled in the study

Serial number	Variable	Diabetic control	Diabetic nephropathy	Membranous nephropathy	IgA nephropathy	Healthy control	P value
1	Sample size (n)	13	05	05	02	06	NA
2	Age (yr), mean ± SD	45.11 ± 7.65	47.11 ± 12	51 ± 3.8	49 ± 1.41	46 ± 10.79	0.04
3	Gender (male/female)	8/5	3/2	4/1	2/0	3/3	NA
4	Duration of DM (yr), mean ± SD	10± 6.08	6 ± 5	2.4 ± 2	5.12 ± 6.9	NA	0.06
5	BMI (kg/m2), mean ± SD	24 ± 3.64	24 ± 3.64	26.6 ± 6	23 ± 5.23	26 ± 7.04	0.09
6	Medical condition	DM or DM & HTN both	DM or DM & HTN both	DM or HTN or DM & HTN both	IgA nephropathy	NA	NA

One-way ANOVA was used to compare quantitative variables between different disease groups wherever applicable. DM: Diabetic mellitus; BMI: Body mass index; HTN: Hypertension; IgA: Immunoglobulin A; NA: Not available.

Table 3 Table displaying housekeeping miRNAs and their mean expression intensity in urinary exosomes across five kidney conditions (control and disease)

ID	Transcript ID	Healthy control	Diabetic control	Diabetic nephropathy	Membranous nephropathy	IgA nephropathy
20536748	has-mir-4479	2.036667	2.024615	2.04	1.93	1.985
20537225	hsa-mir-5703	2.536667	2.173077	2.85	2.43	2.715
20534637	hsa-mir-320a	2.295	2.558462	2.026	2.562	2.495
20535940	hsa-mir-320d-2	3.778333	4.185385	3.65	3.536	3.37
20504391	hsa-miR-638	9.026667	8.643077	9	8.762	9.205
20536665	hsa-mir-4417	4.183333	4.378462	3.932	3.504	3.97
20536810	hsa-mir-4530	2.996667	2.891538	2.886	2.468	2.525
20536248	hsa-mir-3153	2.561667	3.033846	2.242	2.53	2.58
20522537	hsa-miR-5787	9.41	9.218462	9.228	8.386	8.77
20518446	hsa-miR-3921	2.045	2.076923	2.46	2.12	1.395
20536956	hsa-mir-4722	2.78	2.642308	2.768	2.358	2.49
20536702	hsa-mir-4449	2.178333	1.791538	2.69	2.364	2.925
20537581	hsa-mir-6836	2.701667	2.528462	2.566	2.272	2.025
20515578	hsa-miR-3157-3p	2.071667	2.483846	1.772	2.256	1.44