Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Nephrol. Sep 25, 2024; 13(3): 99105
Published online Sep 25, 2024. doi: 10.5527/wjn.v13.i3.99105
Reference gene panel for urinary exosome-based molecular diagnostics in patients with kidney disease
Deendayal D Mishra, Pramod K Maurya, Swasti Tiwari
Deendayal D Mishra, Pramod K Maurya, Swasti Tiwari, Department of Molecular Medicine and Biotechnology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226014, Uttar Pradesh, India
Author contributions: Mishra DD performed the experiments, reviewed the literature, analyzed the data, and wrote the manuscript; Maurya PK performed the experiments, analyzed the data, and wrote the manuscript; Tiwari S designed the study, analyzed the data, and wrote and revised the manuscript; All the authors have read and approved the final version of the manuscript.
Supported by the Indian Council of Medical Research, Coord/7 (1)/CAREKD/2018/NCD-II, No. 5/4/7-12/13/NCD-II; DDM was supported by a fellowship from the Council of Scientific and Industrial Research, India, No. 619/(CSIR-UGC NET DEC. 2018).
Institutional review board statement: The study was reviewed and approved by the Sanjay Gandhi Postgraduate Institute of Medical Sciences Institutional Review Board [Ref No. 2021-194-PhD-EXP-41].
Informed consent statement: Participants were enrolled in the study after written informed consent.
Conflict-of-interest statement: All the authors declare that they have no conflict of interest.
Data sharing statement: Data used in the manuscript is available from the corresponding author at Tiwaris@sgpgi.ac.in.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Swasti Tiwari, PhD, Professor, Department of Molecular Medicine and Biotechnology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Mawaiya, Lucknow 226014, Uttar Pradesh, India. tiwaris@sgpgi.ac.in
Received: July 13, 2024
Revised: August 27, 2024
Accepted: August 30, 2024
Published online: September 25, 2024
Processing time: 67 Days and 12.9 Hours
Core Tip

Core Tip: Early prevention and detection of end-stage renal disease is currently inadequate. miRNAs found in urinary exosomes (UEs) may serve as early biomarkers for kidney diseases. However, the lack of reference miRNAs for normalization makes it difficult to interpret the data from UEs. In this study, we profiled the miRNAs in UEs from 31 human urine samples and identified a set of consistently expressed miRNAs that could be utilized as reference gene biomarkers for kidney disease.