Evolution of an avian H5N1 influenza A virus escape mutant

Figure 1 Phylogenetics of the viral genes of escape mutant H5N1 compared to selected influenza viruses. Escape mutant F10 strain examined in the current study was marked by a red color. The selected viruses were chosen to be representative from relevant sequences in GenBank database: H5N1 strains representative to the major gene lineages. Serotypes H1N1, H1N2, H3N2, H3N8, H7N3, H7N7 and H9N2 were also included in the phylogenetic trees of PB2, PB1, PA, NP, M and NS. For NA, Egyptian N1 sequences from Egyptian H5N1 strains were the only included sequences. The robustness of the individual nodes of the tree was assessed using a bootstrap of 1000 resembling in percent (70% and higher). Influenza A virus genotype tool (http://www.flugenome.org/ genotyping.php) was used to determine individual gene segment lineage. The genotype of each strain was mentioned in a blue color in the phylogenetic trees of PB2, PB1, PA, NP, M and NS.