Rapid genotyping of human rotavirus using SYBR green real-time reverse transcription-polymerase chain reaction with melting curve analysis
Yupin Tong, Department of Medicine, University of Alberta, Edmonton, Alberta T6G 2J2, Canada
Bonita E Lee, Department of Pediatrics, University of Alberta, Edmonton, Alberta T6G 2J2, Canada
Xiaoli L Pang, Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta T6G 2J2, Canada
Xiaoli L Pang, Provincial Laboratory for Public Health, University of Alberta Hospital, Edmonton, Alberta T6G 2J2, Canada
Author contributions: Tong Y performed the experiments, analyzed the data and wrote the manuscript; Lee BE contributed data and sample selections for the study and revised the manuscript; Pang XL designed the research and revised the manuscript.
Institutional review board statement: The study was reviewed and approved by the Provincial Laboratory for Public Health Edmonton Alberta (Provlab) Institutional Review Board.
Conflict-of-interest statement: None.
Data sharing statement: Technical appendix, clinical dataset is available from the corresponding author at email@example.com.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Xiaoli L Pang, PhD, Provincial Laboratory for Public Health, University of Alberta Hospital, WMC 2B4.58, 8440 112 Street, Edmonton, Alberta T6G 2J2, Canada. firstname.lastname@example.org
Telephone: +1-780-4073483 Fax: +1-780-4078984
Received: May 29, 2015
Peer-review started: June 2, 2015
First decision: June 18, 2015
Revised: August 1, 2015
Accepted: September 29, 2015
Article in press: September 30, 2015
Published online: November 12, 2015