Published online Aug 12, 2015. doi: 10.5501/wjv.v4.i3.265
Peer-review started: January 27, 2015
First decision: March 6, 2015
Revised: March 23, 2015
Accepted: May 7, 2015
Article in press: May 8, 2015
Published online: August 12, 2015
Core tip: Rapid development and commercial availability of next-generation sequencers (NGS) systems have dramatically changed almost every field of biological research, especially microbiology and metagenomics. Different NGS systems have been adapted and used for numerous applications in virology too. These systems are capable of rapidly sequencing and analyzing a complex mixture of nucleic acid templates, in a massively parallel fashion, making them ideal tools for viral metagenomics and discovery. This manuscript reviews the prevailing NGS technologies, their application in virus discovery to serve as a guide for the readers, working in the field of virology, public health and in biothreat mitigation programs.