PI3K/AKT/GSK3β regulatory axis in bone mesenchymal stem cells initiates diabetic myocardial infarction via miR-142-3p

Figure 1 Bones mesenchymal stem cells with low expression of miR-142-3p prepare. A: Expression of green fluorescent protein in low expression miR-142-3P bones mesenchymal stem cells (BMCs) (40 ×); B: Apoptosis of flow cytometry and quantitative results of BMCs with low expression of miR-142-3p. ^aP < 0.05 vs the control group; KD: Knockdown; NC: Empty plasmid control.

Figure 2 PI3K/AKT/GSK3β regulatory axis and ferroptosis-related proteins expression and quantitative results of bones mesenchymal stem cells. A: PI3K, AKT and GSK3β WB blots and Grayscale quantitative results with image of bones mesenchymal stem cells (BMCs); B: VEGFR2 and CCM3 WB blots and Grayscale quantitative results with Image of BMCs. ^aP < 0.05, ^bP < 0.01 vs the control group; KD: Knockdown; NC: Empty plasmid control.

Figure 3 Myocardial infarction results of each group of rats. A: He electrocardiogra test results of each group of rats; B: Cardiac gross observation results of rats in each group; BMCs: Bones mesenchymal stem cells.

Figure 4 Blood glucose and myocardial infarction case omics results of each group of rats. A: Blood glucose change results of rats in each group; B: Hematoxylin and eosin staining of rat heart tissue in each group; KD: Knockdown; NC: Empty plasmid control.

Figure 5 Sirius red staining results of rat heart tissue in each group. A: Results of Sirius red staining slices; B: The quantitative analysis results of sirius red staining. ^aP < 0.05, ^dP < 0.0001 vs the control group; BMCs: Bones mesenchymal stem cells.

Figure 6 Masson staining results of rat hearts in each group. A: Results of Masson staining slices; B: Quantitative analysis results of Masson staining of rat hearts in each group. ^cP < 0.001, ^dP < 0.0001 vs the control group; BMCs: Bones mesenchymal stem cells.

Figure 7 TTC staining results of rat hearts in each group. A: Results of TTC staining slices; B: Quantitative results of cardiac TTC staining of rats in each group. ^cP < 0.001, ^dP < 0.0001 vs the control group; BMCs: Bones mesenchymal stem cells.

Figure 8 Results of western blotting expression of PI3K/AKT/GSK3 pathway in heart tissue of rats in each group. A: The PI3K, AKT and GSK3of western blotting (WB) blots in heart tissue of rats in each group; B-D: The PI3K, AKT and GSK3 WB blots of grayscale quantitative results with image in heart tissue of rats in each group. ^cP < 0.001, ^dP < 0.0001 vs the control group; BMCs: Bones mesenchymal stem cells.

Figure 9 Expression of CCM3/VEGFR2 pathway protein in heart tissue of rats in each group and quantitative results. A: The CCM3 and VEGFR2 western blotting (WB) blots in heart tissue of rats in each group; B: The CCM3 and VEGFR2 WB blots of grayscale quantitative results with Image in heart tissue of rats in each group; C: The CCM3 WB blots of grayscale quantitative results with image in heart tissue of rats in each group. ^bP < 0.01, ^cP < 0.001 vs the control group; BMCs: Bones mesenchymal stem cells.

Figure 10  Expression of iron death pathway protein in heart tissue of rats in each group. A: The COX-2, CHCHD6 and GPX4 western blotting (WB) blots in heart tissue of rats in each group; B: The COX-2, CHCHD6 and GPX4 WB blots of grayscale quantitative results with Image in heart tissue of rats in each group; C: The GPX4 WB blots of grayscale quantitative results with image in heart tissue of rats in each group. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs the control group; BMCs: Bones mesenchymal stem cells.