Das P, Chaudhary DK, Mishra R, Tiwari S. Evaluation of urinary extracellular vesicles and microRNAs to diagnose urogenital tuberculosis. World J Exp Med 2025; 15(3): 105208 [DOI: 10.5493/wjem.v15.i3.105208]
Corresponding Author of This Article
Swasti Tiwari, PhD, Professor, Department of Molecular Medicine and Biotechnology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Mawaiya, Lucknow 226014, Uttar Pradesh, India. tiwari.pgi@gmail.com
Research Domain of This Article
Infectious Diseases
Article-Type of This Article
Case Control Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Exp Med. Sep 20, 2025; 15(3): 105208 Published online Sep 20, 2025. doi: 10.5493/wjem.v15.i3.105208
Evaluation of urinary extracellular vesicles and microRNAs to diagnose urogenital tuberculosis
Parijat Das, Dharmendra K Chaudhary, Richa Mishra, Swasti Tiwari
Parijat Das, Dharmendra K Chaudhary, Department of Molecular Medicine and Biotechnology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow 226014, Uttar Pradesh, India
Richa Mishra, Department of Microbiology, Sanjay Gandhi Postgrad Institute Medical Sciences, Lucknow 226014, Uttar Pradesh, India
Swasti Tiwari, Department of Molecular Medicine and Biotechnology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226014, Uttar Pradesh, India
Author contributions: Das P carried out data extraction; Das P and Chaudhary DK performed data curation; Das P and Mishra R contributed to formal analysis; Tiwari S and Mishra R contributed to methodology, reviews and editing; Tiwari S carried out conceptualization and fund acquisition; Das P, Chaudhary DK, and Tiwari S wrote the article; All authors contributed to the interpretation of the study and approved the final version to be published.
Supported by the Indian Council of Medical Research, No. Coord/7 (1)/CARE-KD/18-NCD-II.
Institutional review board statement: This study was approved by the Institutional Ethics Committee of Sanjay Gandhi Postgraduate Institute of Medical Sciences, approval No. PGI/BE/25/2023.
Informed consent statement: Subjects have signed informed consent.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
STROBE statement: The authors have read the STROBE Statement-checklist of items, and the manuscript was prepared and revised according to the STROBE Statement-checklist of items.
Data sharing statement: The data supporting the findings of this study are available from the corresponding author upon reasonable request.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Swasti Tiwari, PhD, Professor, Department of Molecular Medicine and Biotechnology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Mawaiya, Lucknow 226014, Uttar Pradesh, India. tiwari.pgi@gmail.com
Received: January 15, 2025 Revised: April 22, 2025 Accepted: June 20, 2025 Published online: September 20, 2025 Processing time: 210 Days and 0.3 Hours
Core Tip
Core Tip: Discovery of new biomarkers from easily attainable bodily fluids is essential for controlling Urogenital tuberculosis (TB), a significant health concern. Urinary extracellular vesicle microRNAs (miRNAs) are exciting as biomarkers given their stability and non-invasive nature. miRNA-155, miRNA-26 and miRNA-29 play a crucial role in mycobacterium TB pathogenesis via regulation of posttranscriptional gene expression and subsequently apoptosis, cellular proliferation, differentiation and several other biological functions governing various facets of innate and acquired immunity. Thus, there is scope for developing miRNA studies using in vitro and in vivo models in TB which would likely lead to new technologies in diagnosis and treatment.
