Case Report Open Access
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Hematol. Feb 25, 2022; 9(1): 6-12
Published online Feb 25, 2022. doi: 10.5315/wjh.v9.i1.6
Efficacy of HA330-II column hemoadsorption in Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis combined with liver failure: A case report
Qian Gao, You-Peng Jin, Pediatric Intensive Care Unit, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, Shandong Province, China
Xiao-Wei Xin, Chun Zhao, Yu-Juan Wang, Wei Wang, Yi Yin, Xiao-Ru Wang, You-Peng Jin, Pediatric Intensive Care Unit, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong Province, China
ORCID number: Qian Gao (0000-0002-3261-6139); Xiao-Wei Xin (0000-0002-3216-9375); Chun Zhao (0000-0002-5405-9177); Yu-Juan Wang (0000-0003-3103-7626); Wei Wang (0000-0002-6031-8702); Yi Yin (0000-0002-4411-6000); Xiao-Ru Wang (0000-0003-0386-6160); You-Peng Jin (0000-0002-6357-2810).
Author contributions: Material preparation and data collection were performed by Jin YP and Gao Q; the first draft of the manuscript was written by Gao Q; all authors read and approved the final manuscript; all authors contributed to the case report conception and design.
Supported by Clinical Science and Technology Innovation Scheme of Jinan Province, No. 202019177 and No. 201704071.
Informed consent statement: Informed written consent was obtained from the patient’ parents for publication of this report and any accompanying images.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
CARE Checklist (2016) statement: The authors have read the CARE Checklist (2016), and the manuscript was prepared and revised according to the CARE Checklist (2016).
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: You-Peng Jin, PhD, Chief Doctor, Pediatric Intensive Care Unit, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No. 324 Jingwu Road, Huaiyin District, Jinan 250021, Shandong Province, China. jinyp79@sina.cn
Received: September 29, 2021
Peer-review started: September 29, 2021
First decision: December 2, 2021
Revised: December 9, 2021
Accepted: February 12, 2022
Article in press: February 12, 2022
Published online: February 25, 2022
Processing time: 146 Days and 23 Hours

Abstract
BACKGROUND

Hemophagocytic lymphohistiocytosis (HLH) is a severe and potentially deadly condition associated with extensive inflammation and immune activation. Cytokine adsorption may serve as a supportive treatment that can stabilize organ function in affected patients by reducing their circulating cytokines levels. To date, no descriptions of clinical experiences associated with the use of HA330-II column hemoadsorption for the treatment of children affected by HLH have been published.

CASE SUMMARY

We describe the case of an 11-year-old child with Epstein-Barr virus-associated HLH complicated by liver failure. She underwent HA330-II column hemoadsorption and chemotherapy and exhibited reductions in levels of inflammatory cytokines, including interleukin (IL), IL-6, IL-8, IL-10, and interferon-γ. The patient’s condition and laboratory parameters gradually improved with treatment.

CONCLUSION

Hemoadsorption may play an important role in cytokine storm elimination in children with HLH combined with liver failure and consequent multiple organ failure.

Key Words: Hemoadsorption; HA330-II column; Hemophagocytic lymphohistiocytosis; Pediatric; Liver failure; Case report

Core Tip: Hemophagocytic hymphohistiocytosis (HLH) is an often fatal disease. We report an 11-year-old female who was diagnosed with Epstein-Barr virus-HLH and presented with coagulation disorders, liver damage, and respiratory insufficiency. In the present case, initially elevated interleukin (IL)-6, IL-8, IL-10, and interferon-γ levels were reduced to within normal ranges following hemoadsorption with HA330-II, and the patient’s condition gradually improved. HA330-II hemoadsorption has the ability to bridge the patient until chemotherapy can contribute to reduced HLH disease activity.



INTRODUCTION

Hemophagocytic lymphohistiocytosis (HLH) is a severe and potentially lethal disorder associated with excessive inflammation and unrestrained immune activation[1]. Patients with HLH exhibit dramatically elevated levels of cytokines, including interleukin (IL)-1, IL-2, IL-6, IL-18, tumor necrosis factor-α, and interferon (IFN)-γ[2]. HLH is classified into two groups: Primary and acquired. Primary HLH primarily develops as a consequence of genetic defects during infancy, while acquired HLH occurs in the context of auto-inflammatory/autoimmune diseases, lymphoma, or certain viral infections, with Epstein-Barr virus (EBV) being a common cause. Animal studies[3,4] and case series have demonstrated that a reduction in circulating cytokine levels achieved via hemoadsorption can be effective as a treatment for HLH[5,6]. HA330-II perfusion columns have been reported to be capable of absorbing multiple inflammatory factors and have been successfully used as a component of a double plasma molecular adsorption system to treat patients suffering from liver failure[7,8]. In the present article, we describe one case of a child diagnosed with EBV-associated HLH combined with liver failure who successfully underwent HA330-II column hemoadsorption and chemotherapy treatment. Through these treatments, the patient’s condition improved and her recovery was satisfactory.

CASE PRESENTATION
Chief complaints

An 11-year-old female was admitted to our hospital with a 5 d history of high fever with chills and a headache as well as damaged liver function. She did not exhibit a cough, abdominal pain, or vomiting.

History of present illness

Before this visit, she had been given a penicillinase antibiotic for 3 d and azithromycin for 1 d. Her clinical state rapidly deteriorated, and she developed respiratory failure, capillary leak syndrome, and hypotension. As such, she was admitted to the pediatric intensive care unit.

History of past illness

The patient had a history of encephalitis 3 years ago, and she had recovered after treatment for 2 wk.

Personal and family history

This patient had no specific personal or family history.

Physical examination

On initial examination, the patient had a fever with a maximal temperature of 40.0 °C, a respiratory rate of 30 breaths per min, a heart rate of 122 bpm, and a blood pressure of 82/41 mmHg at admission. She was in a poor mental state and presented with jaundice and hepatosplenomegaly. Other physical examinations were normal.

Laboratory examinations

Initial laboratory studies in the pediatric intensive care unit revealed excessive hyperferritinemia (58360 ng/mL, reference range: 11.0-306.8 ng/mL), low natural killer cell activity (0.32%, reference range: 5%-26%), hypofibrinogenemia (0.84 g/L, reference range: 1.50-4.35 g/L), leukopenia (2.07 × 109/L, reference range: 3.5-9.5 × 109/L), neutropenia (0.92 × 109/L, reference range: 1.8-6.3 × 109/L) elevated international normalized ratio values (1.92, reference range: 0.8-1.2), thrombocytopenia (42 × 109/L, reference range: 125-350 × 109/L), elevated alanine transaminase (ALT) levels (921 U/L, reference range: 7-40 U/L), aspartate aminotransferase (AST) levels (2223 U/L, reference range: 13-35 U/L), and elevated total bilirubin (TBIL) levels (108.7 mmol/L, reference range: 3.5-23.5 mmol/L). The patient also exhibited high levels of C reactive protein (76.40 mg/L, reference range: 0-8 mg/L), procalcitonin (2.73 ng/mL, reference range: 0-0.05 ng/mL), IL-6 (154.06 pg/mL, reference range: 0-5.4 pg/mL), IL-8 (32.67 pg/mL, reference range: 0-20.6 pg/mL), IL-10 (169.81 pg/mL, reference range: 0-12.9 pg/mL), and IFN-γ (4387.41 pg/mL, reference range: 0-23.1 pg/mL). EBV-DNA loads were also found to be significantly elevated (3.82 × 106 copies/mL). Multiple blood and sputum cultures as well as the other viral polymerase chain reaction tests for common respiratory viruses and cytomegalovirus were all negative. A bone marrow biopsy revealed the presence of hemophagocytosis.

Imaging examinations

A thoracic-abdominal computer tomography analysis revealed pulmonary inflammation and no evidence of tumors.

FINAL DIAGNOSIS

She was diagnosed with EBV-associated hemophagocytic syndrome combined with liver failure in accordance with the HLH-2004 guidelines[9].

TREATMENT

Treatment with meropenem, norepinephrine, intravenous immunoglobulin, ganciclovir, and dexamethasone as well as high-flow nasal cannula placement were initiated. However, these approaches were ineffective as evidenced by sustained fever, hypoxemia, and hypotension. Chemotherapy was recommended for the patient, but her parents refused and asked for other therapeutic options. In light of the refractory state of her HLH and her poor general condition, we next sought to achieve the immediate suppression of hypercytokinemia. Accordingly, we initiated blood purification. Plasma exchange was initially considered but could not be performed owing to reduced plasma separator access due to the coronavirus disease 2019 pandemic. As such, we tried to perform hemoadsorption (HA330-II perfusion column, Zhuhai Health Sails Biotechnology Co., Ltd., Zhuhai, China) in this patient. Informed written consent was obtained from the patient’s parent. Heparin sodium was employed for anticoagulation, and the patient was infused with platelets and fibrinogen prothrombin complex concentrate.

On the 1st day of hospitalization, she was in poor condition with respect to her clinical symptoms and biochemical parameters. She also needed a high dose of norepinephrine to maintain appropriate cardiovascular function. On the 2nd day of hospitalization, one round of the above mentioned hemoadsorption strategy was implemented. This hemoadsorption approach was implemented two more times over a 3-d period. On the 5th day of hospitalization, the patient exhibited significantly decreased levels of IL-6 (12.16 pg/mL), IL-8 (10.63 pg/mL), IL-10 (63.38 pg/mL), and IFN-γ (61.99 pg/mL) (Figure 1). She was gradually weaned off norepinephrine treatment, and her fever disappeared while her total leukocyte and neutrophil counts increased. However, no significant improvement in liver function was observed (ALT 766 U/L, AST 1196 U/L, TBIL 170.54 mmol/L, Fib 1.23 g/L), and inflammatory markers rebounded after hemoadsorption had been discontinued for 2 d, at which time the patient again developed a fever that reached as high as 40.0 C. At that time, her parents provided consent for chemotherapy (HLH-2004) treatment (Figure 2), which was initiated in combination with hemoadsorption.

Figure 1
Figure 1 Interleukin 6 and interleukin 10 levels, and interferon-γ and interleukin 8 levels under treatment during the first 13 d after pediatric intensive care unit admission. A: Interleukin 6 and interleukin 10 levels; B: Interferon-γ and interleukin 8 levels. IL: Interleukin; NE: Norepinephrine; HLH: Hemophagocytic lymphohistiocytosis; IFN: Interferon.
Figure 2
Figure 2 Chemotherapy protocol for the treated patient.
OUTCOME AND FOLLOW-UP

The hemoadsorption approach was implemented an additional three times over a 5-d period, and the patient’s condition gradually improved. On the 13th day of hospitalization, decreased levels of IL-10 (30.06 pg/mL) and IFN-γ (50.69 pg/mL) (Figure 1), improved liver function (ALT 257 U/L, AST 393 U/L, TBIL 79.91 mmol/L, Fib 1.45 g/L) (Figure 3), and increased platelet counts were all evident. She was discharged on the 40th day after admission owing to her good recovery status. The patient underwent an additional 30 d of chemotherapeutic treatment without significant adverse events. Furthermore, no disease recurrence was evident as of 8 mo post-discharge.

Figure 3
Figure 3 Changes in laboratory values during the first 13 d after pediatric intensive care unit admission. AST: Aspartate aminotransferase; ALT: Alanine aminotransferase; LDH: Lactate dehydrogenase.
DISCUSSION

HLH is an often fatal disease, and affected patients typically present with high-grade fever, progressive hypocytosis, liver dysfunction, and coagulopathy[9]. In our case, the patient presented with a persistent fever that had been present for more than 1 wk, hypocytosis, hypofibrinogenemia, splenomegaly, hyperferritinemia, and lymphohistiocytic accumulation in the bone marrow. HLH was thus diagnosed in this child. The most common treatment for HLH at present is chemotherapy[9]. However, when patients also present with serious organ damage, chemotherapy may not work, as the existence of severe multiorgan failure at presentation can lead to a high mortality rate[10]. At admission, our patient was in poor condition and presented with coagulation disorders, liver damage, and respiratory insufficiency. In such a context, cytokine adsorption has the potential to bridge the patient until chemotherapy can contribute to reduced HLH disease activity[5].

The primary pathophysiological characteristics of HLH include excessive cytotoxic T cell and macrophage activation and expansion. These activated immune cells, in turn, produce excessively high levels of inflammatory cytokines, including IL-1, IL-2, IL-6, IL-10, TNF-α, and IFN-γ, which can promote further cytotoxic T cell and macrophage activation and expansion, thereby exacerbating the ongoing cytokine storm and driving consequent multiple organ failure[2]. On admission, patients present with increased circulating levels of certain inflammatory cytokines, such as IL-6, IL-8, IL-10, and IFN-γ, suggesting an ongoing systemic inflammatory reaction. HA330-II is a neutral microporous resin column with abundant micropores and a high specific surface area[11]. HA330-II cartridges have the ability to adsorb medium and large-sized inflammatory cytokines, including those of the IL and TNF families. In the present case, initially elevated IL-6, IL-8, IL-10, and IFN-γ levels were reduced to within normal ranges following hemoadsorption with HA330-II. This indicated that this column was able to reduce effectively inflammatory cytokines levels in our treated patient. However, this approach was unable to remediate effectively HLH-related liver failure in this patient, whereas hemoadsorption combined with chemotherapy was found to be more effective than hemoadsorption alone.

With respect to hemoadsorption, the CytoSorbTM column or endotoxin-binding polymyxin B-immobilized fiber column hemoadsorption approaches have also been reported for the treatment of HLH[12,13]. Cytokine adsorption associated with the CytoSorbTM column results in an improvement in the condition of treated patients, thus aiding in achieving symptom relief in affected individuals. Polymyxin B-immobilized fiber columns have also contributed to the recovery of circulatory dynamics associated with HLH. Several recent studies have demonstrated that HA330-II hemoadsorption plays a critical part in the elimination of inflammatory cytokines[14,15]. To the best of our knowledge, this is the first report to describe the clinical application of an HA330-II perfusion column in children suffering from HLH. However, as this is a report of outcomes for a single patient, large-scale trials will be necessary to investigate the clinical indications for such hemoadsorption treatment.

CONCLUSION

In summary, for EBV-associated HLH, HA330-II column-mediated hemoadsorption can safely reduce the levels of circulating inflammatory cytokines, serving as a beneficial and essential supplement to chemotherapy.

ACKNOWLEDGEMENTS

We are grateful to the reviewers and editor for their constructive comments.

Footnotes

Provenance and peer review: Unsolicited article; Externally peer reviewed.

Peer-review model: Single blind

Specialty type: Hematology

Country/Territory of origin: China

Peer-review report’s scientific quality classification

Grade A (Excellent): 0

Grade B (Very good): 0

Grade C (Good): C, C

Grade D (Fair): 0

Grade E (Poor): 0

P-Reviewer: Bendari M, Navarrete Arellano M S-Editor: Guo XR L-Editor: Filipodia P-Editor: Cai YX

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