Specificity in the alteration of lesional and non-lesional skin lipids in atopic dogs

Figure 1 Chromatographic plate with high performance thin-layer of free and protein-bound ceramides from the Stratum corneum of healthy dog skin, Labrador breed. Standards: Left lane; CerNS (ceramide with normal fatty acid and sphingosine): Upper spot; CerAS (ceramide with alpha hydroxy fatty acid and sphingosine): Lower spot; Center lane CerNP (ceramide with normal fatty acid and phytosphingosine); CerEOS: Ceramide with omega esterified fatty acid and sphingosine; CerNS: Ceramide with normal fatty acid and sphingosine; CerAS: Ceramide with alpha-hydroxyl fatty acid and sphingosine; CerNH: Ceramide with normal fatty acid and 6-hydroxysphingosine; CerAP: Ceramide with alpha-hydroxyl fatty acid and phytosphingosine; CerNP: Ceramide with normal fatty acid and phytosphingosine.

Figure 2 Free ceramides in the stratum corneum of healthy dogs (1, 2) and an atopic dog, non-lesional (3) and lesional areas (4). Standard lipids on the left lanes of the plates: type III and type IV ceramides, phytoceramides.

Figure 3 High performance thin-layer chromatography of protein-bound ceramides from non-lesional (A) and lesional (B) stratum corneum of atopic dog (strip tapes taken by two before extraction. Standard ceramide Cer III (CerAS). Figure 3A reproduced from ref.[30] (strips tapes pooled two by two before extraction).

Figure 4 High performance thin-layer chromatography of protein-bound glucosylceramides from non-lesional (B) and lesional (C) stratum corneum of atopic dog compared to healthy dog (A) (strips tapes by two before extraction. Std- mixture of glucosylceramide (CMH) and lactosylceramide (CDH). Figures 4A and 4B reproduced from ref.[30] (strips tapes pooled two by two before extraction).

Figure 5 High performance thin-layer chromatography of protein-bounds ceramides from SC of non-lesional skin of an atopic dog before (A) and after treatmanet with Megaderm R (B) (tapes pooled two by two before extraction). Standard- CerOS.