Copyright
©The Author(s) 2025.
World J Orthop. Jul 18, 2025; 16(7): 107045
Published online Jul 18, 2025. doi: 10.5312/wjo.v16.i7.107045
Published online Jul 18, 2025. doi: 10.5312/wjo.v16.i7.107045
Table 1 Oligonucleotides used in this study
qPCR primer | Sequence |
miR-365 forward primer | TAATGCCCCTAAAAATCCTTAT |
miR-365 reverse primer | CAAGCAGAAGACGGCATACG |
Sox9 forward primer | AGAATGAGAGCGGCGGAGACAA |
Sox9 reverse primer | CTCTTTCTCCAGTTCCAGGGTC |
Kdm6a forward primer | AGCGCAAAGGAGCCGTGGAAAA |
Kdm6a reverse prime | GTCGTTCACCATTAGGACCTGC |
Hoxa9 forward primer | AGAATGAGAGCGGCGGAGACAA |
Hoxa9 reverse primer | CTCTTTCTCCAGTTCCAGGGTC |
Hif-1α forward primer | TATGAGCCAGAAGAACTTTTAGGC |
Hif-1α reverse primer | CACCTCTTTTGGCAAGCATCCTG |
β-actin forward primer | CACCATTGGCAATGAGCGGTTC |
β-actin reverse primer | AGGTCTTTGCGGATGTCCACGT |
siRNA mimics | Sequence |
NC-mimic-sense | UUUGUACUACACAAAAGUACUG |
NC-mimic-antisense | CAGUACUUUUGUGUAGUACAAA |
miR-365-mimic-sense | UAUUCCUAAAAAUCCCCGUAAU |
miR-365-mimic-antisense | AUUACGGGGAUUUUUAGGAAUA |
siRNAs | Sequence (dT, deoxythymidine) |
siNC-sense | GGCUCUAGAAAAGCCUAUGC (dTdT) |
siNC-antisense | GCAUAGGCUUUUCUAGAGCC (dTdT) |
siHoxa9-sense | AGAAUGAGAGCGGCGGAGAC (dTdT) |
siHoxa9-antisense | GUCUCCGCCGCUCUCAUCU (dTdT) |
- Citation: Zhi ZZ, Liu T, Kang J, Zhou FC, Liu XD, He ZM. miR-365 promotes HOXA9-mediated differentiation of mesenchymal stem cells to nucleus pulposus cells by interacting with HIF-1α. World J Orthop 2025; 16(7): 107045
- URL: https://www.wjgnet.com/2218-5836/full/v16/i7/107045.htm
- DOI: https://dx.doi.org/10.5312/wjo.v16.i7.107045