miR-365 promotes HOXA9-mediated differentiation of mesenchymal stem cells to nucleus pulposus cells by interacting with HIF-1α

doi:10.5312/wjo.v16.i7.107045

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Jul 18, 2025 (publication date) through Jul 28, 2025

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World Journal of Orthopedics

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2218-5836

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Orthop. Jul 18, 2025; 16(7): 107045
Published online Jul 18, 2025. doi: 10.5312/wjo.v16.i7.107045

Table 1 Oligonucleotides used in this study

qPCR primer	Sequence
miR-365 forward primer	TAATGCCCCTAAAAATCCTTAT
miR-365 reverse primer	CAAGCAGAAGACGGCATACG
Sox9 forward primer	AGAATGAGAGCGGCGGAGACAA
Sox9 reverse primer	CTCTTTCTCCAGTTCCAGGGTC
Kdm6a forward primer	AGCGCAAAGGAGCCGTGGAAAA
Kdm6a reverse prime	GTCGTTCACCATTAGGACCTGC
Hoxa9 forward primer	AGAATGAGAGCGGCGGAGACAA
Hoxa9 reverse primer	CTCTTTCTCCAGTTCCAGGGTC
Hif-1α forward primer	TATGAGCCAGAAGAACTTTTAGGC
Hif-1α reverse primer	CACCTCTTTTGGCAAGCATCCTG
β-actin forward primer	CACCATTGGCAATGAGCGGTTC
β-actin reverse primer	AGGTCTTTGCGGATGTCCACGT
siRNA mimics	Sequence
NC-mimic-sense	UUUGUACUACACAAAAGUACUG
NC-mimic-antisense	CAGUACUUUUGUGUAGUACAAA
miR-365-mimic-sense	UAUUCCUAAAAAUCCCCGUAAU
miR-365-mimic-antisense	AUUACGGGGAUUUUUAGGAAUA
siRNAs	Sequence (dT, deoxythymidine)
siNC-sense	GGCUCUAGAAAAGCCUAUGC (dTdT)
siNC-antisense	GCAUAGGCUUUUCUAGAGCC (dTdT)
siHoxa9-sense	AGAAUGAGAGCGGCGGAGAC (dTdT)
siHoxa9-antisense	GUCUCCGCCGCUCUCAUCU (dTdT)

Citation: Zhi ZZ, Liu T, Kang J, Zhou FC, Liu XD, He ZM. miR-365 promotes HOXA9-mediated differentiation of mesenchymal stem cells to nucleus pulposus cells by interacting with HIF-1α. World J Orthop 2025; 16(7): 107045
URL: https://www.wjgnet.com/2218-5836/full/v16/i7/107045.htm
DOI: https://dx.doi.org/10.5312/wjo.v16.i7.107045