World J Orthop. Nov 18, 2026; 17(11): 118182 Published online Nov 18, 2026. doi: 10.5312/wjo.118182
Osteoreparative potential of nanofibrillated cellulose, xenograft, and butvar-phenolic adhesive-based composite
Berik E Tuleubayev, Bakhtiyar K Makhatov, Vladimir A Vinokurov, Yevgeniy K Kamyshanskiy, Yekaterina Y Kossilova, Saule B Akhmetova, Amina A Koshanova, Marat Z Davletbaev, Marietta G Arutyunyan
Berik E Tuleubayev, Amina A Koshanova, Department of Surgical Diseases, Karaganda Medical University, Karaganda 100000, Kazakhstan
Bakhtiyar K Makhatov, Yekaterina Y Kossilova, Marat Z Davletbaev, Marietta G Arutyunyan, Department of Surgery Diseases, NPJSC “Karaganda Medical University”, Karaganda 100000, Kazakhstan
Vladimir A Vinokurov, Department of Physical and Colloidal Chemistry, Federal State Autonomous Educational Institution of Higher Education Gubkin Russian State University of Oil and Gas, Moscow 119991, Moskva, Russia
Yevgeniy K Kamyshanskiy, Pathology Unit of the University Clinic, Karaganda Medical University, Karaganda 100000, Kazakhstan
Saule B Akhmetova, Department of Biomedicine, NPJSC “Karaganda Medical University”, Karaganda 100000, Kazakhstan
Co-first authors: Berik E Tuleubayev and Bakhtiyar K Makhatov.
Author contributions: Tuleubayev BE, Akhmetova SB, Davletbaev MZ, Arutyunyan MG and Vinokurov VA designed the research study; Makhatov BK, Kamyshanskiy YK, Koshanova AA, Kossilova YY performed the research; Tuleubayev BE and Makhatov BK made crucial and indispensable contributions towards the completion of the project and thus qualified as the co-first authors of the paper.
Supported by The Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan, No. АР19678427.
Institutional animal care and use committee statement: The study was conducted according to the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (2010) and approved by the Local Ethics Committee of Karaganda Medical University (No. 18).
Conflict-of-interest statement: The authors declare that there is no conflict of interest regarding the publication of this paper.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: All data analyzed during this study are included in this article.
Corresponding author: Bakhtiyar K Makhatov, Doctorate Student, Department of Surgery Diseases, NPJSC “Karaganda Medical University”, Gogol Street 40, Karaganda 100000, Kazakhstan. mahatov@qmu.kz
Received: December 26, 2025 Revised: February 6, 2026 Accepted: October 8, 2026 Published online: November 18, 2026 Processing time: 319 Days and 11.3 Hours
Core Tip
Core Tip: This preclinical study investigates a tricomponent bone substitute comprised of nanofibrillated cellulose, thermally disinfected bone xenograft (Marburg Bone Bank System), and polyvinyl butyral-phenolic (Butvar-phenolic) adhesive implanted as a uniformly premixed composite in a rat femoral diaphyseal defect model. The mixed composite demonstrated superior cortical plate regeneration on days 30 and 60, significantly reduced intramedullary migration, and more pronounced scaffold remodeling compared with xenograft alone. In a Staphylococcus aureus-inoculated wound model, the composite markedly attenuated osteonecrosis and polymorphonuclear granulocyte infiltration. These results support its dual role as an osteoconductive/osteoinductive scaffold with inherent barrier resistance to infection-related bone injury.
