Xue-Zhen Zheng, Hong-Yan Yu, Jian-Sheng Fang, Department of Anesthesiology, The First People's Hospital of Chun'an County, Hangzhou 311700, Zhejiang Province, China
Ye-Ru Chen, Department of Anaesthesiology, Sir Run Run Shaw Hospital of Zhejiang University, Hangzhou 310058, Zhejiang Province, China
Co-corresponding authors: Xue-Zhen Zheng and Jian-Sheng Fang.
Author contributions: Zheng XZ and Fang JS designed the study; Zheng XZ and Yu HY prepared the samples, performed the experiments, and analyzed the data; Chen YR and Fang JS wrote the original manuscript; all authors read and approved the final manuscript.
Supported by National Natural Science Foundation of China, No. 82001424.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Zhejiang University Experimental Animal Ethics Committee Institutional Review Board (Approval No. ZJU20240012).
Conflict-of-interest statement: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: The data generated in the present study may be requested from the corresponding author. The data generated in the present study are included in the figures and/or tables of this article.
Corresponding author: Xue-Zhen Zheng, MD, Department of Anesthesiology, The First People's Hospital of Chun'an County, No. 1869 Huanhu North Road, Qiandaohu Town, Chun'an County, Hangzhou 311700, Zhejiang Province, China. zhengxuezhen2024@163.com
Received: December 4, 2024 Revised: January 26, 2025 Accepted: February 24, 2025 Published online: May 15, 2025 Processing time: 142 Days and 1.6 Hours
Core Tip
Core Tip: Our research demonstrates that aucubin markedly reduces pain and anxiety-like behavior in Streptozotocin-induced diabetic mice. Furthermore, aucubin reinstates microglial aerobic glycolysis and mitigates inflammation. Utilizing the SwissTargetPrediction database, we discovered 22 prospective protein targets for aucubin concerning diabetic neuropathic pain (DNP). Analysis of protein-protein interaction networks and molecular docking experiments identified the potential role of aldose reductase (AKR1B1) to streptozotocin therapy. Aucubin does not repair aerobic glycolysis and inflammation in the setting of AKR1B1 depletion. The findings indicate that aucubin could be a potential therapeutic drug for DNP, with AKR1B1 identified as a major target.
