Original Article
Copyright ©2013 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Diabetes. Dec 15, 2013; 4(6): 339-348
Published online Dec 15, 2013. doi: 10.4239/wjd.v4.i6.339
Changes of phasic and tonic smooth muscle function of jejunum in type 2 diabetic Goto-Kakizaki rats
Jing-Bo Zhao, Peng-Min Chen, Hans Gregersen
Jing-Bo Zhao, Department of Gastroenterology and Surgery, Aalborg University Hospital, DK 9000 Aalborg, Denmark
Peng-Min Chen, Department of Molecular Biology, Institute of Clinical Medicine, China-Japan Friendship Hospital, Beijing 100029, China
Jing-Bo Zhao, Hans Gregersen, The College of Bioengineering, Chongqing University, Chongqing 400044, China
Hans Gregersen, The GIOME Institute, GIOME FZE, Ras Al-Khaimah 6300, United Arab Emirates
Author contributions: Zhao JB and Chen PM designed the research, performed the experiment, analyzed the data and wrote the manuscript; Gregersen H participated in the study design and revised the manuscript and English grammar.
Supported by Karen Elise Jensens Foundation
Correspondence to: Jing-Bo Zhao, Associate Professor, Mech-Sense, Department of Gastroenterology and Surgery, Aalborg University Hospital, Sdr. Skovvej 15, DK9000 Aalborg, Denmark. jz@rn.dk
Telephone: +45-993-26907 Fax: +45-993-26801
Received: May 1, 2013
Revised: September 15, 2013
Accepted: October 17, 2013
Published online: December 15, 2013
Processing time: 232 Days and 20.9 Hours
Abstract

AIM: To generate phasic and tonic stress-strain curves for evaluation of intestinal smooth muscle function in type 2 diabetic rats during active and passive conditions.

METHODS: Seven diabetic Goto-Kakizaki (GK) male rats, 32-wk old (GK group), and 9 age-matched normal Wistar rats (Normal group) were included in the study. Jejunal segments were distended up to a pressure of 10 cm H2O in an organ bath containing 37 °C Krebs solution with addition of carbachol (CA). The pressure and outer diameter changes were synchronously recorded. Passive conditions were obtained using calcium-free Krebs solution containing ethylene glycol tetraacetic acid and papaverine. Total phasic, tonic and passive circumferential stress and strain were computed from the diameter and pressure data with reference to the zero-stress state geometry. The active phasic and tonic stresses were defined as the total phasic and tonic stresses minus the passive stress.

RESULTS: Diabetes increased jejunal mucosa and muscle layer thicknesses compared to the Normal group (mucosa, 755.8 ± 63.3 vs 633.1 ± 59.1 μm, P < 0.01; muscle, 106.3 ± 12.9 vs 85.2 ± 11.7 μm, P < 0.05). The pressure and stress thresholds were decreased in the GK group after CA application compared to distensions without CA application (pressure, 1.01 ± 0.07 vs 1.99 ± 0.19 cmH2O, P < 0.01; stress, 0.11 ± 0.01 vs 0.24 ± 0.02 kPa, P < 0.01). CA application did not change the pressure and stress threshold in the Normal group (pressure, 2.13 ± 0.32 vs 2.34 ± 0.32 cm H2O, P > 0.05; stress, 0.25 ± 0.03 vs 0.35 ± 0.06 kPa, P > 0.05). The amplitude of total phasic, total tonic, active phasic and active tonic circumferential stresses did not differ for the distensions without CA application between the GK group and the Normal group. However, the total phasic and total tonic stresses increased after CA application in the GK group compared those in the Normal group. When normalized to muscle layer thickness, the amplitude of active stresses before CA application was lowest in the GK group compared with the Normal group. No difference was found during CA application.

CONCLUSION: The stress generated by intestinal muscle normalized to the muscle layer thickness was lowest in GK rats compared to normal rats whereas the response to CA stimulation was preserved.

Keywords: Intestine; Diabetes; Muscle function; Stress-strain curves; Carbachol; Rat

Core tip: Length-tension diagrams of smooth muscle strips were obtained in intact segment of intestine in vitro in the present study. We demonstrated that it is a valid tool to evaluate smooth muscle function in intact intestine. Diabetes decreased the force (stress) generated by the smooth muscle normalized to the muscle layer thickness. Since the stress decreased and the muscle layer thickness increased in diabetic rats, it indicates that the intestine, at least in part, remodels in a stress-dependent way. Furthermore, the smooth muscle in Goto-Kakizaki diabetic intestine preserved its response to carbachol stimulation.