Oxocrebanine inhibits the proliferation of hepatocellular carcinoma cells by promoting apoptosis and autophagy

Table 1 Growth inhibition rate of Hep3B2.1-7 cells in MTT assay (mean ± SD, n = 3)

Group	Concentration (μmol/L)	Growth inhibition rate (%)
Oxocrebanine	5	4.18 ± 0.02
	10	10.24 ± 0.01a
	20	25.09 ± 0.03a
	40	35.59 ± 0.02a
	80	76.6 ± 0.02a

^aP < 0.01 vs control.

Table 2 Proliferation level of Hep3B2.1-7 cells in BrdU assay (mean ± SD, n = 3)

Group	Concentration (μmol/L)	Proliferation rate (%)
Oxocrebanine	0	102.6 ± 0.07
	5	95.6 ± 0.04a
	10	88.59 ± 0.05a
	20	79.09 ± 0.02a
	40	60.24 ± 0.02a
	80	37.18 ± 0.06a

^aP < 0.01 vs control.

Table 3 Proliferation level of Hep3B2.1-7 cells in plate colony formation assay (mean ± SD, n = 3)

Group	Concentration (μmol/L)	Proliferation rate (%)
Control	-	100
VP16	32.54	49.76 ± 0.02a
Oxocrebanine	25.45	76.30 ± 0.02a
	50.89	51.74 ± 0.04a
	101.78	23.55 ± 0.03a

^aP < 0.01 vs control.

Table 4 Apoptosis rate of Hep3B2.1-7 cells in different groups (mean ± SD, n = 3)

Group	Concentration (μmol/L)	Apoptosis rate (%)
Control	-	22.40 ± 0.02
VP16	32.54	49.76 ± 0.02b
Oxocrebanine	25.45	26.30 ± 0.02a
	50.89	31.74 ± 0.04b
	101.78	53.55 ± 0.03b

^aP < 0.05 vs control.

^bP < 0.01 vs control.

Table 5 Hep3B2.1-7 cell viability and proliferation rate in MTT assay and BrdU test (mean ± SD, n = 3)

Group	Cell viability (%)	Proliferation rate (%)
Control	100	101.60 ± 0.07
3-MA	91.68 ± 0.04a	92.60 ± 0.03a
Oxo	48.56 ± 0.09a	60.22 ± 0.06a
Oxo + 3-MA	26.37 ± 0.05a,b	49.09 ± 0.05a,b

^aP < 0.01 vs control.

^bP < 0.01 vs oxocrebanine group.