Circulating exosomal miR-17-92 cluster serves as a novel noninvasive diagnostic marker for patients with gastric cancer

Table 1 Primer sequences used for qRT-PCR analysis

Gene	Sequence (5′-3′)
miR-16	CAGCACGTAAATATTGGCGA
miR-17	GCAAAGTGCTTACAGTGCAGGTAG
miR-18	CCGGTAAGGTGCATCTAGT
miR-19a	TGTGCAAATCTATGCAAAACTG
miR-19b	GCATCCCAGTGTGCAAATCC
miR-20	GGTAAAGTGCTTATAGTGCAGGTAG
miR-92	ATTGCACTTGTCCCGGCCTGT

Table 2 Correlation between the expression levels of the serum exosomal miR-17-92 cluster and clinical pathological parameters in patients with gastric cancer

Variable	No. of patients (n = 72)	miR-17 expression	P value	miR-18 expression	P value	miR-19a expression	P value	miR-92 expression	P value
Age (years)
< 60	30	14.083 (11.920-16.247)	0.188	10.297 (8.681-11.913)	0.629	11.383 (9.945-12.822)	0.117	31.007 (26.953-35.060)	0.34
≥ 60	42	16.179 (13.968-18.389)		10.793 (9.473-12.112)		9.864 (8.585-11.144)		28.633 (25.534-31.733)
Sex
Male	39	16.021 (14.083-17.958)	0.323	10.659 (9.408-11.910)	0.876	10.255 (8.832-11.677)	0.643	28.936 (25.170-32.701)	0.544
Female	33	14.460 (11.867-17.054)		10.500 (8.822-12.178)		10.703 (9.369-12.036)		30.433 (27.359-33.508)
Tumor size (cm)
< 5	46	13.600 (11.773-15.427)	0.003b	9.774 (8.547-11.001)	0.03a	10.602 (9.456-11.748)	0.772	29.585 (26.414-32.756)	0.968
≥ 5	26	18.323 (15.718-20.928)		12.023 (10.348-13.699)		10.312 (8.523-12.101)		29.688 (25.697-33.680)
Histological grade
Well-moderately differentiated	32	15.075 (12.525-17.625)	0.794	10.463 (8.935-11.990)	0.827	9.725 (8.216-11.234)	0.149	28.931 (25.705-32.158)	0.615
Poorly differentiated	40	15.490 (13.468-17.512)		10.685 (9.306-12.064)		11.115 (9.877-12.353)		30.175 (26.531-33.819)
Tumor depth
T1-T2	35	13.613 (11.290-15.972)	0.036a	9.946 (8.494-11.397)	0.216	9.197 (5.529-10.457)	0.007b	27.006 (23.425-30.586)	0.036a
T3-T4	37	16.889 (14.852-18.926)		11.192 (9.778-12.606)		11.727 (10.378-13.076)		32.097 (28.851-35.344)
Lymph node metastasis
Absent	24	15.121 (11.663-18.574)	0.883	9.938 (8.007-11.868)	0.364	9.071 (7.624-10.518)	0.033a	27.521 (23.749-31.293)	0.225
Present	48	15.398 (13.720-17.076)		10.910 (9.722-12.098)		11.21 (9.999-12.422)		30.673 (27.509-33.837)
Venous invasion
Negative	51	15.448 (12.109-18.786)	0.908	10.282 (9.005-11.560)	0.348	10.682 (9.474-11.890)	0.511	29.159 (26.302-32.016)	0.557
Positive	21	15.247 (13.452-17.042)		11.324 (9.762-12.886)		10.048 (8.494-11.602)		30.748 (25.785-35.710)

^aP < 0.05.

^bP < 0.01.