Prognostic value of vascular endothelial growth factor receptor 1 and class III β-tubulin in survival for non-metastatic rectal cancer

Table 1 Patient characteristics

Characteristics	Data, n (%)
Gender
Female	36 (48)
Male	39 (52)
Age (yr)
median (range)	52 (29-74)
≤ 60	58 (77.3)
> 60	17 (22.7)
Pre-CEA (ng/mL)
≤ 5	36 (63.2)
> 5	21 (36.8)
Pre-Hb (g/L)
≤ 120	26 (34.7)
> 120	49 (65.3)
Distance to verge (cm)
≤ 5	46 (61.3)
> 5	29 (38.7)
T stage
T1 + T2	13 (17.3)
T3 + T4	63 (82.6)
Lymph node metastasis
Negative	22 (29.3)
Positive	53 (70.6)
Venous invasion
Negative	68 (90.7)
Positive	7 (9.3)
Tumor differentiation
Poorly differentiated	20 (26.7)
Moderately-well differentiated	55 (73.3)
Chemotherapy
No	9 (12)
Yes	66 (88)
TUBB3 expression
Low-expression	39 (52)
High-expression	36 (48)
VEGFR1 expression
Low-expression	53 (70.7)
High-expression	22 (29.3)
TUBB3 and VEGFR1
Both low expression	32 (42.6)
Others	43 (57.3)

Pre-CEA: Preoperative carcino-embryonic antigen; Pre-Hb: Preoperative hemoglobin.

Table 2 Correlation between vascular endothelial growth factor receptor 1 and class III β-tubulin expression with clinicopathological features

	TUBB3			VEGFR1
Parameter	Low (n)	High (n)	P	Low (n)	High (n)	P
Gender			0.426			0.081
Female	17	19		22	14
Male	22	17		31	8
Age (yr)			0.31			1
≤ 60	32	26		41	17
> 60	7	10		12	5
Pre-CEA			0.203			0.244
≤ 5	20	16		26	10
> 5	8	13		12	9
Pre-Hb			0.801			0.206
≤ 120	13	13		16	10
> 120	26	23		37	12
Distance to verge (cm)			0.608			0.792
≤ 5	25	21		32	14
> 5	14	15		21	8
T stage			0.883			0.744
T1 + T2	7	6		10	3
T3 + T4	32	30		43	19
Lymph node metastasis			0.071			0.013
Negative	15	7		20	2
Positive	24	29		33	20
Tumor thrombus			0.25			1
Negative	37	31		48	20
Positive	2	5		5	2
Tumor differentiation			0.754			0.939
Poorly	11	9		14	6
Moderately-well	28	27		39	16
Chemotherapy			0.156			0.051
No	7	2		9	0
Yes	32	34		44	22
VEGFR1			0.024
Low	32	21
High	7	15

Pre-CEA: Preoperative carcino-embryonic antigen; Pre-Hb: Preoperative-hemoglobin.

Table 3 Cox regression analysis for overall survival

Variables	Univariate			Multivariate
	HR	95%CI	P	HR	95%CI	P
Gender
Female/male	1.018	0.519-1.997	0.958
Age
≤ 60/> 60	1.175	0.548-2.518	0.679
Pre-CEA
≤ 5/> 5	1.067	0.496-2.298	0.868
Pre-Hb
≤ 120/> 20	0.651	0.328-1.290	0.219
Distance to verge (cm)
≤ 5/> 5	1.265	0.642-2.491	0.497
T stage
T1 + T2/T3 + T4	4.221	1.011-17.632	0.048	4.05	0.968-116.93	0.055
Lymph node metastasis
Negative/positive	6.247	1.905-20.491	0.003	3.042	1.137-8.142	0.027
Tumor thrombus
Negative/positive	1.303	0.458-3.705	0.62
Tumor differentiation
Poorly/moderately-well	0.503	0.251-1.006	0.052	-	-	0.18
Chemotherapy
No/yes	1.407	0.430-4.605	0.572
TUBB3 expression
Low/high	2.407	1.188-4.877	0.015	-	-	0.1
VEGFR1 expression
Low/high	2.817	1.424-5.570	0.003	2.151	1.062-4.355	0.033

Pre-CEA: Preoperative carcino-embryonic antigen; Pre-Hb: Preoperative hemoglobin.