Published online Oct 15, 2018. doi: 10.4251/wjgo.v10.i10.351
Peer-review started: June 2, 2018
First decision: July 10, 2018
Revised: July 17, 2018
Accepted: August 26, 2018
Article in press: August 26, 2018
Published online: October 15, 2018
To assess the long-term prognostic value of vascular endothelial growth factor receptor 1 (VEGFR1) and class III β-tubulin (TUBB3) mRNA expression in non-metastatic rectal cancer.
A total of 75 consecutive patients with non-metastatic rectal cancer from March 2004 to November 2008 were analyzed retrospectively at our institute. The mRNA expressions of VEGFR1 and TUBB3 were detected by multiplex branched DNA liquid-chip technology. The Cutoff Finder application was applied to determine cutoff point of mRNA expression. SPSS software version 22.0 was used for analysis.
The median follow-up was 102.7 mo (range, 6-153.6). The χ2 and Fisher’s exact tests showed that VEGFR1 expression was related to lymph node metastasis (P = 0.013), while no relationships between TUBB3 and clinicopathological features were observed. Univariate analysis showed that T stage, lymph node metastasis, tumor differentiation, VEGFR1 and TUBB3 mRNA expression were correlated to overall survival (OS) (P = 0.048, P = 0.003, P = 0.052, P = 0.003 and P = 0.015, respectively). Also, lymph node metastasis and VEGFR1 expression independently influenced OS by multivariate analysis (P = 0.027 and P = 0.033). VEGFR1 expression was positively correlated with TUBB3 (P = 0.024). The patients with low expression of both TUBB3 and VEGFR1 presented a better OS (P = 0.003). In addition, the receiver operating characteristic analysis suggested that the combination of lymph node metastasis and VEGFR1 had a more favorable prognostic value (P < 0.001).
VEGFR1 expression and lymph node metastasis independently and jointly affect survival. Moreover, low expression of VEGFR1 and TUBB3 presented a better OS in patients with non-metastatic rectal cancer, which might serve as a potential prognostic factor.
Core tip: Nowadays, personalized and precision medicine becomes vital in cancer treatment. Herein, we focus on the long-term prognostic value of vascular endothelial growth factor receptor 1 (VEGFR1) and class III β-tubulin (TUBB3) mRNA expression in non-metastatic rectal cancer. In the 75 consecutive patients enrolled, we found that VEGFR1 expression and lymph node metastasis were independent factors influencing overall survival, and the combination of them showed a favorable prognostic value. Also, VEGFR1 expression was significantly related to lymph node metastasis. In addition, VEGFR1 expression was positively correlated with TUBB3 expression.