Fajardo CA, Quiroga AJ, Coronado A, Labrador K, Acosta N, Delgado P, Jaramillo C, Bravo MM. CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses. World J Gastrointest Oncol 2013; 5(3): 50-59
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María Mercedes Bravo, MS, Head, Cancer and Infectious Agents Research Group, National Cancer Institute, Bogotá 110411, Colombia. firstname.lastname@example.org
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World J Gastrointest Oncol. Mar 15, 2013; 5(3): 50-59 Published online Mar 15, 2013. doi: 10.4251/wjgo.v5.i3.50
CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses
Carlos Alberto Fajardo, Andrés Javier Quiroga, Andrea Coronado, Karen Labrador, Nicole Acosta, Pilar Delgado, Carlos Jaramillo, María Mercedes Bravo
Carlos Alberto Fajardo, Andrés Javier Quiroga, Andrea Coronado, Karen Labrador, María Mercedes Bravo, Cancer and Infectious Agents Research Group, National Cancer Institute, Bogotá 110411, Colombia
Nicole Acosta, Pilar Delgado, Carlos Jaramillo, Molecular Diagnostics and Bioinformatics Laboratory, Biological Sciences Department, Los Andes University, Bogotá 111711, Colombia
ORCID number: $[AuthorORCIDs]
Author contributions: Bravo MM and Quiroga AJ designed the study; Fajardo CA, Quiroga AJ, Coronado A, Labrador K and Acosta N performed the research; Fajardo CA, Quiroga AJ, Coronado A, Labrador K, Acosta N, Delgado P, Jaramillo C and Bravo MM analyzed the data; Fajardo CA and Bravo MM wrote the paper.
Supported by National Cancer Institute, Bogotá, Colombia, Grant No. 41030310 to Bravo MM and Sciences Faculty, Los Andes University, Bogotá, Colombia
Correspondence to: María Mercedes Bravo, MS, Head, Cancer and Infectious Agents Research Group, National Cancer Institute, Bogotá 110411, Colombia. email@example.com
Telephone: +57-1-3341111 Fax: +57-1-3341360
Received: August 28, 2012 Revised: December 22, 2012 Accepted: January 14, 2013 Published online: March 15, 2013
AIM: To investigate the influence of the CagA diversity in Helicobacter pylori (H. pylori) strains from Colombia on the host cell biology.
METHODS: Eighty-four H. pylori-cagA positive strains with different Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs patterns, isolated from patients with gastritis (n = 17), atrophic gastritis (n = 17), duodenal ulcer (n = 16), intestinal metaplasia (n = 16) and gastric cancer (n = 18), were included. To determine the integrity of the cag pathogenicity island (cagPAI) we evaluated the presence of cagA, cagT, cagE, and cag10 genes by polymerase chain reaction. AGS gastric epithelial cells were infected with each strain and assayed for translocation and tyrosine phosphorylation of CagA by western blot, secretion of interleukin-8 (IL-8) by enzyme-linked immuno sorbent assay after taking supernatants from cocultures and cell elongation induction. For cell elongation quantification, coculture photographs were taken and the proportion of “hummingbird” cells (> 15 μm) was determined.
RESULTS: Overall 72% (60/84) of the strains were found to harbor a functional cagPAI. Levels of phosphorylated CagA were significantly higher for isolates from duodenal ulcer than the ones in strains from gastritis, atrophic gastritis, intestinal metaplasia and gastric cancer (49.1% ± 23.1% vs 21.1% ± 19.5%, P < 0.02; 49.1% ± 23.1% vs 26.2% ± 14.8%, P < 0.045; 49.1% ± 23.1% vs 21.5% ± 19.5%, P < 0.043 and 49.1% ± 23.1% vs 29.5% ± 27.1%, P < 0.047 respectively). We observed variable IL-8 expression levels ranging from 0 to 810 pg/mL and from 8.8 to 1442 pg/mL at 6 h and 30 h post-infection, respectively. cagPAI-defective strains did not induce detectable levels of IL-8 at 6 h post-infection. At 30 h post-infection all strains induced IL-8 expression in AGS cells, although cagPAI-defective strains induced significantly lower levels of IL-8 than strains with a functional cagPAI (57.1 ± 56.6 pg/mL vs 513.6 ± 338.6 pg/mL, P < 0.0001). We did not observe differences in the extent of cell elongation induction between strains with a functional or a defective cagPAI in 6 h cocultures. At 24 h post infection strains with functional cagPAI showed high diversity in the extent of hummingbird phenotype induction ranging from 7% to 34%. cagPAI defective strains induced significantly lower levels of elongation than strains with functional cagPAI with one or more than one EPIYA-C motif (15.1% ± 5.2% vs 18.9% ± 4.7%, P < 0.03; and 15.1% ± 5.2% vs 20.0% ± 5.1%, P < 0.003 respectively). No differences were observed in cellular elongation induction or IL-8 expression among H. pylori strains bearing one and more than one EPIYA-C motifs, neither at 6 h nor at 24 h of coculture. There were no associations between the levels of induction of cell elongation or IL-8 expression and number of EPIYA motifs or pathology.
CONCLUSION: The present work describes a lack of association between H. pylori CagA protein EPIYA motifs variations from Colombian isolates and disease-associated cellular responses.
Citation: Fajardo CA, Quiroga AJ, Coronado A, Labrador K, Acosta N, Delgado P, Jaramillo C, Bravo MM. CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses. World J Gastrointest Oncol 2013; 5(3): 50-59
Helicobacter pylori (H. pylori) infects more than 50% of the world’s population. This pathogen has been associated with the development of chronic gastritis, duodenal ulcers and gastric cancer, and was classified as a type Icarcinogen by the International Agency for Research on Cancer.
One of the most important virulence factors of H. pylori is the cag pathogenicity island (cagPAI), which encodes for a type IV secretion system (T4SS). Also encoded in the cagPAI is the CagA protein, which is translocated into gastric epithelial cells through the T4SS, where it undergoes phosphorylation by members of the SRC and Abl families of kinases on tyrosine residues within the C-terminal Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs[6-8]. Phosphorylated CagA interacts with the cellular phosphatase SHP-2, which in turn activates several signaling pathways involved, among others, in actin cytoskeletal rearrangements, leading to cell elongation (also known as the “hummingbird phenotype”)[10,11]. Translocated CagA can also induce a proinflammatory response, resulting in the expression of interleukin-8 (IL-8) through the activation of nuclear factor κ B (NF-κB)[12-14].
CagA varies in size, and this variation has been shown to be due to EPIYA motifs repeats within the C-terminal region of the protein[15,16]. Four types of EPIYA motifs have been described (A, B, C and D) based on the sequence flanking the motif. Western H. pylori isolates have shown to harbor combinations of type A, B and C motifs, while East Asia isolates harbor combinations of type A, B and D motifs[17,18].
A positive association between the number of EPIYA motifs repeats and the phosphorylation of CagA protein has been reported[17,19]. Several studies have shown that strains with higher numbers of EPIYA-C motifs are more closely associated with gastric cancer[20-22].
IL-8 expression in gastric tissue has been reported to correlate with the histopathological severity in H. pylori-positive patients[23,24]. Furthermore, it has been shown that strains with higher number of EPIYA-C motifs significantly increased IL-8 expression in gastric epithelial cells. As with IL-8 expression, CagA proteins with higher number of EPIYA motifs, and especially EPIYA-C motifs, have shown to potentiate cell elongation in AGS cells[17,19,26,27].
The aim of this study was to evaluate the possible association between CagA EPIYA motifs variations in H. pylori isolates from Colombia with the phosphorylation of CagA protein, the expression of IL-8 and cell elongation induction in gastric epithelial cells. Associations between disease severity and H. pylori-induced cellular responses in vitro were also evaluated.
MATERIALS AND METHODS
H. pylori strains
In total, 84 cagA-positive and 6 cagPAI-negative strains obtained from the stock collection at the Instituto Nacional de Cancerología, in Bogotá, Colombia were included in the study. CagA-positive strains were isolated from patients diagnosed with gastritis (n = 17), atrophic gastritis (n = 17), duodenal ulcer (n = 16), intestinal metaplasia (n = 16) and gastric cancer (n = 18). Isolates’cagA genotyping was reported previously and EPIYA motifs combinations used in this study are summarized in Figure 1. cagA-positive reference strain NCTC 11 637, with an ABCCC polymorphism, was used as a positive control.
Figure 1 Helicobacter pylori CagA Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs variations included in this study.
Eighty-four cagA-positive strains isolated from Colombian patients were evaluated. All strains possessed Western EPIYA motifs (A, B and C) ranging from 2 to 5 in number.
Detection of cagPAI genes
H. pylori genomic DNA was obtained from plate cultures of each isolate using DNAzol (Invitrogen) extraction method according to the manufacturer’s instructions. The primers used in this study are listed in Table 1. To determine the integrity of cagPAI we evaluated the presence of cagA, cagT, cagE and cag10 genes. All PCR reactions were performed in a volume of 25 μL containing 10 mmol/L Tris, 50 mmol/L KCl, 1.5 mmol/L MgCl2, 200 μmol/L dNTPs, 25 pmol of the primers, 100 ng of H. pylori genomic DNA and 1U Taq polymerase. The polymerase chain reaction (PCR) conditions for each reaction were previously described[29,30]. Positive (strain 11637) and negative controls (strain 3062) for the cagPAI were included in each run. PCR products were analyzed by agarose gel electrophoresis with ethidium bromide staining.
Table 1 Primers used for the detection of CagPAI genes.
Product size (bp)
Culture of H. pylori strains
H. pylori strains were grown on blood agar plates, supplemented with 7% horse serum (Invitrogen), 1% Vitox (Oxoid), and Campylobacter selective supplement (Oxoid), at 37 °C in a 10% CO2-humidified atmosphere for 3 d. Grown plates were subcultured into brucella broth (DIFCO) containing 10% horse serum (Invitrogen) and Campylobacter selective supplement (Oxoid), and were incubated under microaerophilic and shaking conditions for 24 h. Overnight cultures were set to an optical density of 0.1 at 600 nm (approximately 1.2 × 108 bacteria/mL) by dilution. Brucella broth was discarded after centrifugation of liquid cultures at 7000 rpm for 10 min and bacteria were resuspended in serum- and antibiotic-free RPMI medium (GIBCO) prior to infection.
AGS epithelial cells were seeded into 6-well plates (4 × 105 cells/well) or 25 cm2 flasks (5 × 105 cells) and grown in RPMI 1640 (GIBCO) supplemented with 10% fetal bovine serum (GIBCO), 100 U/mL penicillin (Invitrogen), 100 μg/mL streptomycin (Invitrogen) and 2.5 µg/mL amphotericin (GIBCO) at 37 °C in a 5% CO2 atmosphere for 24 h. Eighty percent confluent cell cultures were then washed with phosphate buffered saline (PBS), and serum- and antibiotic-free RPMI was added to the wells. Sixteen hours serum-starved cell cultures were infected with H. pylori suspensions at a multiplicity of infection (MOI) of 100. Cocultures were incubated at 37 °C in a 5% CO2-humidified atmosphere.
CagA phosphorylation assays
After 6 h of coculture the medium was removed and cells were washed with PBS containing 1.0 mmol/L CaCl2 and 0.5 mmol/L MgCl2 and scraped from the flasks into 3 mL PBS containing 1 mmol/L sodium vanadate, harvested by centrifugation at 1000 g by 10 min, resuspended in 100 μL of PBS-sodium vanadate and lysed with 4 × Laemli sample buffer [0.2 TRIS-HCl pH 6.8, 0.4 mmol/L ditiothreitol, 8% sodium dodecyl sulfate (SDS), 40% glycerol, 0.4% bromophenol blue]. Cell lysates were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis using a 6% resolving gel and a 4% stacking gel. Proteins were then transferred onto nitrocellulose membranes by semidry transfer. For protein detection, membranes were probed with a 1:1000 dilution of anti-phospho-tyrosine monoclonal antibody (Santa Cruz Biotechnologies) followed by a 1:4000 dilution of HRP-conjugated goat anti-mouse (Zymax, Invitrogen). Blots were developed using the Amersham ECL detection reagents (GE Healthcare). Membranes were subsequently stripped (using a 62.5 mmol/L TRIS-HCL pH 6.8, 100 μmol/L ß-2-Mercaptoethanol solution, 2% SDS at 50 °C for 30 min) and reprobed with 1:1000 polyclonal anti-CagA antibody (Santa Cruz Biotechnologies) followed by 1:60 000 HRP-conjugated goat anti-rabbit secondary antibody (Zymax, Invitrogen) and developed as described above. Densitometry was performed using a Gel Doc GS-670 (Biorad) and results were expressed as the ratio of phosphorylated CagA to total CagA multiplied by 100.
Medium samples from 6 h and 30 h co-cultures were collected, centrifuged at 7000 rpm for 10 min to discard unattached bacteria or cells, and supernatants were stored at -80 °C until further use. IL-8 concentration was measured using an IL-8 Human ELISA kit (Invitrogen) according to the manufacturer’s instructions. Uninfected AGS cells were used as a negative control.
Cellular elongation assay
Six hours and twenty-four h cocultures were examined by differential interference contrast microscopy with a Leica DM IL phase contrast inverted microscope (Leica). For this, 3 randomly chosen 20 × fields were photographed with a MD800-CK camera for microscope (Amscope). Hummingbird cells were measured and counted with the software ImageJ v1.44c (developed by Wayne Rasband at the National Institutes of Health, Bethesda, MD, United States and available at http://rsb.info.nih.gov/ij/). Hummingbird cells are characterized by the formation of needle-like projections. We defined hummingbird phenotype as cells with needle-like projections > 15 μm. Uninfected AGS cells were used as a negative control.
Mann Whitney U test was used for statistical analysis. A P value < 0.05 was considered statistically significant. All data were analyzed with the software Graphpad Prism 5 (Graphpad Software, Inc.). All experiments were run in duplicates.
cagPAI status, CagA expression and tyrosine phosphorylation
From the 84 cagA-positive strains, 74 (88.1%) tested positive for cagE, 72 (85.7%) for cagT and 68 (81%) for cag10. Overall, 67 (79.8%) tested positive for all four cagPAI genes by PCR, and were therefore predicted, on the basis of this limited testing, to have an intact cagPAI. The remaining 17 strains, which tested negative for one or more genes, were collectively predicted to have a partial (i.e., incomplete) cagPAI. Bacterial lysates of the 84 strains were assessed by Western blot with anti-CagA antibodies, from which 75 (89.3%) expressed the CagA protein. The nine strains lacking CagA expression harbored a partial cagPAI.
Once CagA is expressed, it is delivered into host cells via the T4SS and becomes phosphorylated by host cell kinases. Seventy-three out of the seventy-five CagA-expressing strains were evaluated for CagA phosphorylation in coculture with AGS cells (Figure 2). From these, in 58 strains (79.4%) CagA was phosphorylated during infection. In 15 strains CagA was not phosphorylated, including eight CagA-expressing strains bearing a partial cagPAI. The remaining seven strains lacking CagA phosphorylation were predicted to have an “intact”cagPAI according to cagT, cagE, and cag10 PCR results. This last result indicates that PCR detection of selected cagPAI genes is not sufficient to predict the functionality of the cagPAI. In addition, and as described below, these seven strains failed to induce IL-8 secretion in AGS cells, which supports this conclusion. Based on this in vitro characterization of the strains, we grouped isolates bearing a partial cagPAI (i.e., strains which tested negative for one or more cagPAI genes) and strains with a non-functional cagPAI (i.e., strains showing no CagA phosphorylation nor induction of IL-8 secretion) as strains with a defective cagPAI. In summary, 24 out of the 84 strains (28.5%) were found to harbor a defective cagPAI: 17 strains with a partial cagPAI and 7 strains with a non-functional cagPAI.
Figure 2 Phosphorylation of CagA protein in AGS cells after coculture with Helicobacter pylori CagA-positive strains.
Cell lysates were evaluated by western blot using anti-phosphotyrosine or anti-CagA antibodies. A representative assay is shown. Lane 1: 11 637 control strain with a functional cagPAI and 5 Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs (ABCCC); Lanes 2 and 6: Isolates with a defective cagPAI lacking expression of CagA; Lanes 7 and 8: Isolates with a defective cagPAI expressing CagA, with absence of CagA phosphorylation; Lanes 3-5: Isolates with a functional CagPAI with three (ABC; lane 3) or four (ABCC; lanes 4 and 5) EPIYA motifs; Lane 9: Uninfected AGS cells.
We further evaluated the association degree between the levels of CagA phosphorylation and the histopathological diagnoses for strains with functional cagPAI. Interestingly, the mean of CagA phosphorylation in strains from duodenal ulcer was shown to be significantly higher than the ones in strains from gastritis, atrophic gastritis, intestinal metaplasia and gastric cancer (P < 0.02, 0.045, 0.043 and 0.047 respectively; Figure 3A).
Figure 3 CagA-protein phosphorylation and its relationship to Glu-Pro-Ile-Tyr-Ala-C (EPIYA-C) motifs and disease severity.
Sixty functional-cagPAI strains were cocultured with AGS cells for 6 h. Coculture lysates were assessed by Western blot and levels of CagA phosphorylation were determined by densitometry. A: Evaluation of CagA phosphorylation levels according to the pathology from which strains were isolated; B: Relationship between the number of EPIYA motifs and the levels of CagA phosphorylation. G: Gastritis; DU: Duodenal ulcer; AG: Atrophic gastritis; IM: Intestinal metaplasia; GC: Gastric cancer. NS: Not significant
We also investigated the relationship between the number of EPIYA-C motifs and the levels of CagA phosphorylation. Isolates from this study ranged from one to three EPIYA-C motifs (Figure 1). CagA-expressing H. pylori strains bearing one and more than one EPIYA-C motifs were grouped together. Although higher levels of CagA phosphorylation were observed in strains with more than one EPIYA-C motif in comparison with strains with one EPIYA-C motif, this difference was not significant (Figure 3B).
Influence of cagPAI status and cagA polymorphisms on IL-8 expression in AGS cells
The 84 cagA-positive and 6 cagPAI-negative strains isolated from Colombian patients were tested for IL-8 induction in AGS cells. CagA-positive strains induced variable expression levels of IL-8 ranging from 0 to 810 pg/mL and from 8.8 to 1442 pg/mL at 6 h and 30 h post-infection, respectively. Ten out of the 67 strains classified by genotyping to bear an intact cagPAI did not induce IL-8 expression after 6 h of coculture with AGS cells. Three of these strains showed CagA translocation and phosphorylation in contrast to the remaining seven strains, in which no phosphorylation was observed. These last seven strains were likely to bear defects in other cagPAI components not detected by the PCR of the selected cagPAI genes. This showed, as previously observed by Argent et al, that PCR prediction of cagPAI intactness is a poor test for the presence of a T4SS capable of inducing IL-8 expression in AGS. We therefore considered these strains to have a non-functional cagPAI and classified them, along with the partial-cagPAI strains, as cagPAI-defective isolates, as described above.
CagPAI-negative and cagPAI-defective strains did not induce detectable levels of IL-8 at 6 h post-infection (Figure 4A). At 30 h post-infection all strains induced IL-8 expression in AGS cells, although cagPAI-negative and cagPAI-defective strains induced significantly lower levels of IL-8 than strains with a functional cagPAI with one or more than one EPIYA-C motif (P < 0.001; Figure 4B).
Figure 4 Influence of cagPAI status and CagA Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs variations on interleukin-8 expression and cell elongation.CagPAI-negative strains (n = 6), cagPAI-defective strains (n = 24) and functional-cagPAI strains with either one (n = 40) or more than one (n = 20) EPIYA-C motifs were cocultured with AGS cells. (A) 6 h and (B) 30 h coculture supernatants were collected and assessed for interleukin-8 concentration by ELISA. Mean values are represented by horizontal lines within the scatterplots. Experiments for each strain were run in duplicates; (C) 6 h and (D) 24 h coculture photographs were taken and the percentage of hummingbird cells was determined. Mean values are represented by horizontal lines within the scatterplots. Experiments for each strain were run in duplicates. NS: Not significant.
A previous report has suggested a positive association between the number of EPIYA-C motifs and IL-8 expression. We therefore evaluated H. pylori-IL-8 induction according to the number of EPIYA-C motifs in each strain. There were no differences in IL-8 expression among H. pylori strains bearing one and more than one EPIYA-C motifs, neither at 6 h nor at 30 h of coculture, suggesting a lack of association between CagA EPIYA-C motifs variations in H. pylori isolates from Colombia and IL-8 induction (Figure 4A and B).
Influence of cagPAI status and cagA polymorphisms on hummingbird phenotype induction
AGS cells were cocultured with the same 84 strains tested for IL-8 expression and evaluated for hummingbird phenotype formation. We did not observe differences in the extent of cell elongation induction between strains with a functional or a defective cagPAI in 6 h cocultures (Figure 4C). At 24 h post infection strains with functional cagPAI showed high diversity in the extent of hummingbird phenotype induction ranging from 7% to 34%. CagPAI-negative and cagPAI-defective strains induced significantly lower levels of elongation than strains with functional cagPAI with one or more than one EPIYA-C motif (P = 0.032 and 0.003 respectively; Figure 4D).
Similarly to IL-8 expression, no differences were observed in cellular elongation induction among H. pylori strains bearing one and more than one EPIYA-C motifs, neither at 6 h nor at 24 h of coculture (Figure 4C and D). Unexpectedly, three cagPAI-defective strains induced elongation in more than 20% of the cells.
H. pylori-induced cellular responses and their association to disease severity
To assess the degree of association between the disease severity and IL-8 or cell elongation induction, strains with a functional cagPAI were grouped by the pathology from which they were isolated.
No differences were found in IL-8 expression among pathology groups, although small variations among IL-8 mean values were observed (Table 2). A slight increase in IL-8 mean values after 30 h of coculture in the direction Atrophic Gastritis, Intestinal Metaplasia, Gastric Cancer was observed. However, differences among groups were not significant. Interestingly, the two strains that showed the highest induction were isolated from patients diagnosed with gastric cancer.
Table 2 Induction of interleukin-8 expression and cell elongation by cagPAI-functional Helicobacter pylori strains according to the histopathological diagnosis.
Hummingbird phenotype induction had no significant association to disease severity either (Table 2). As in IL-8 induction, the two strains showing the highest cell elongation induction belonged to the gastric cancer group. However, these two strains were different from those inducing the highest IL-8 levels.
In Colombia, a country with high incidence of gastric cancer, 72% to 90% of H. pylori isolates harbor the cagA gene[31,32], a virulence factor associated with more severe disease. It has been shown that the number of EPIYA-C motifs on CagA is associated with the levels of CagA tyrosine phosphorylation, SHP-2 binding activity and cytoskeletal alterations[17,26]. In this study we have evaluated the biological activities of cagA-positive Colombian strains on gastric epithelial cells according to the CagA polymorphisms, and their potential association with the severity of gastroduodenal diseases.
Although we included 84 cagA-positive strains in this study, the presence of this gene did not show strict concordance with the integrity of cagPAI, nor with the expression and delivery of CagA into epithelial AGS cells. About 28% of the strains were found to have a defective cagPAI, and 10%, in addition of being cagPAI-defective, did not express the CagA protein. It is likely, that the promoter region of the cagA gene was disrupted in these strains, as previously reported for isolates from different human populations. These results reinforce previous reports indicating that the presence of the cagA gene alone is not an accurate marker for an intact cagPAI[19,34-36].
Strains bearing an intact cagPAI showed high variability in CagA phosphorylation levels. In vitro experiments have shown that the number of EPIYA-C motifs is associated with the degree of CagA phosphorylation, and some studies with clinical isolates have also reported this association[19,27]. In our study we observed higher levels of CagA phosphorylation in strains with more than one EPIYA-C motifs than in strains with one EPIYA-C motif, although the differences were not significant. Considering that it has been proposed that CagA EPIYA motifs polymorphisms influence the degree of virulence as well as the oncogenic potential of individual cagA-positive strains, we evaluated the association between phosphorylation levels and histopathological diagnosis. We observed significant higher levels of CagA phosphorylation in strains from duodenal ulcer patients when compared to strains from the other pathologies, which is in agreement with a previous study reporting a similar behavior in strains isolated from duodenal ulcer patients. It has been shown that H. pylori-induced inflammatory response is triggered upon CagA translocation into the host cell, where it activates NF-κB leading to IL-8 expression[12,13]. Furthermore, CagA-mediated IL-8 induction has been shown to be time- and strain-dependent. There is evidence demonstrating the importance of CagA for IL-8 expression in long incubation periods (24-48 h)[12,25]. However, the role of CagA in short incubation periods has been controversial. One study has found that isogenic cagA-mutant strains induce lower levels of IL-8 expression than their parental strains after 6 h and 9 h of co-culture. In contrast, two studies, one involving isogenic cagA mutants and the second involving independent strains, found that IL-8 expression was not affected after 6 h of incubation[19,38]. Given these contradictory results, we tested IL-8 induction after 6 h and 30 h post-infection. We observed a clear CagA-dependent IL-8 expression pattern, as evidenced by the differences in IL-8 induction between cagA-negative/cagPAI-defective and cagPAI-functional strains. Strains bearing a functional cagPAI induced variable levels of IL-8 expression at 6 h of coculture, whereas cagA-negative and cagPAI-defective strains failed to induce IL-8 secretion. Furthermore, we confirmed the importance of CagA on IL-8 induction after long incubation periods, although we also detected low levels of IL-8 expression for cagA-negative and cagPAI-defective strains. These low levels of IL-8 induction at 30 h are probably the effect of other delayed responses like the CagA-independent IL-8 expression mechanism, in which H. pylori peptidoglycan translocated through bacterial membrane vesicles into epithelial cells activates, via Nod1, NF-κB resulting in IL-8 expression[39,40]. Furthermore, Crabtree et al also reported low levels of secretion of IL-8 by cagA-negative strains after 24 h of infection. Taken together, our results support the concept of H. pylori time-dependent IL-8 induction, highlighting the importance of CagA for both, short and long, incubation periods.
We observed a lack of association between the number of EPIYA-C motifs and the level of IL-8 induction after coculture with AGS cells, even in prolonged incubation times. Neither an increasing number of EPIYA motifs nor an increasing number of EPIYA-C motifs had a boost effect on IL-8 expression. There are reports in the literature supporting our findings[42-44]. Reyes-Leon et al reported no differences in IL-8 induction between Mexican H. pylori strains bearing one EPIYA-C motif and those with two or more C motifs. Interestingly, Mexican and Colombian H. pylori populations share common predominant polymorphisms (ABC and ABCC)[28,43,45]. Moreover, Sgouras et al observed no differences in the levels of secreted IL-8 induced by individual isogenic subclones expressing CagA protein with different number of EPIYA-C motifs isolated from the same patient. It is worth noting the contrast of our results with those found by Argent et al, in which they observed a direct association between the number of EPIYA-C motifs and IL-8 expression in microevolved H. pylori strains from England. Discrepancies between studies may be explained by contrasting the geographical origin of the strains in each study. Our strains were isolated in Colombia and Argent strains were isolated in England, which are regions with high and mild gastric cancer risks, respectively. It has recently been shown that H. pylori strains from different geographical and gastric cancer risk regions have distinct IL-8 induction behaviors in AGS cells. Cellular inflammatory response in AGS cells was shown to be independent of the pathology from which strains were isolated, although the two strains showing the highest IL-8 expression levels were isolated from patients diagnosed with gastric cancer. These results are in agreement with previous studies showing that H. pylori strains isolated from different gastric pathologies varied in their ability to induce IL-8 expression in AGS cells, but did not associate to disease severity[19,47]. Moreover, Schneider et al reported in a recent study involving cagA-positive H. pylori strains isolated from Colombian patients, that IL-8 expression induced by isolates from precancerous lesions did not differ from that induced by isolates from nonatrophic gastritis.
The induction of hummingbird phenotype upon infection with cagA-positive H. pylori strains has long been proposed as one of the mechanisms contributing to CagA oncogenic transformation[8,17]. Strains carrying biologically more active CagA have been associated with an increased risk of developing gastric carcinoma[17,19,22]. H. pylori-mediated cell elongation is potentiated by CagA proteins with higher number of EPIYA motifs[19,27]. Furthermore, proteins harboring higher number of EPIYA-C repeats increase hummingbird cells in AGS cells[17,26]. Our results disagree with these statements, as neither of both CagA molecular variations groups affected hummingbird phenotype formation, but are in agreement with previous studies suggesting a lack of association between the number and type of EPIYA motifs and cellular elongation in H. pylori clinical isolates[22,37,43,48].
We found no differences in cell elongation induction when evaluating strains according to the pathology from which they were isolated. These results are in agreement with the results reported by Backert et al, in which strains isolated from German patients with different gastric pathologies showed no differences in cell elongation in AGS cells.
It is also important not to take H. pylori infection as the ultimate factor involved in gastric carcinogenesis, as there are many environmental and host factors associated with the disease. Polymorphisms in cytokine genes, such as IL-8, IL-1β and tumor necrosis factor-α, affect cytokine production upon H. pylori infection, increasing the risk of developing gastric diseases. In addition to host genetic factors, environmental factors (e.g., dietary and smoking habits) may also play an important role in H. pylori pathogenesis[49,50]. More interestingly, a recent study based on epidemiological and geographical data has proposed altitude as a surrogate for host, bacterial and environmental factors associated with gastric cancer risk.
In conclusion, we have reported a lack of association between H. pylori CagA protein EPIYA motifs variations from Colombian isolates and disease-associated cellular effects. Taken together, these results suggest that other factors (e.g., host or environmental) may play a more important role than H. pylori CagA protein EPIYA variations in gastric cancer development in Colombia.
Helicobacter pylori (H. pylori) infect more than 50% of the world’s population. Around 10%-15% of the infected individuals develop gastroduodenal diseases such as chronic gastritis, duodenal ulcers and gastric cancer. Currently, the determinants of the variable clinical outcomes have not been fully elucidated. One of the most important virulence factors of H. pylori is the cag pathogenicity island (cagPAI), which encodes several proteins, including CagA.
CagA varies in size, and this variation has been shown to be due to Glu-Pro-Ile-Tyr-Ala (EPIYA) repeats within the C-terminal region of the protein. In Western H. pylori strains three types of EPIYA motifs have been described (A, B and C) based on the sequence flanking the motif. Strains with higher numbers of EPIYA-C motifs are more closely associated with gastric cancer and with an increased CagA in vitro activity, although this is controversial.
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In contrast with studies in other populations, this study reports a lack of association between CagA EPIYA motifs variations from H. pylori colombian isolates and disease-associated cellular responses or gastroduodenal disease severity.
These results suggest that other factors (e.g., host or environmental) may play a more important role than H. pylori CagA protein EPIYA variations in gastric cancer development in Colombia, a country with a high incidence of gastric cancer.
H. pylori CagA protein is a virulence factor encoded in the cagPAI of the bacterium which is translocated into gastric epithelial cells through a type IV secretion system. Once in the cell, CagA becomes phosphorylated on tyrosine residues within the EPIYA motifs, mediating in turn the activation of several signaling pathways involved in the expression of pro-inflammatory cytokines such as interleukin-8 or in cell elongation, among others.
The authors investigates the role of cagA H. pylori gene polymorphisms in the various bacterial-related chronic conditions. The paper is well designed and the results represent novel aspects of the infection consequences.
P- Reviewers Iera E, McGee DJ S- Editor Song XX L- Editor A E- Editor Lu YJ
Kusters JG, van Vliet AH, Kuipers EJ. Pathogenesis of Helicobacter pylori infection.Clin Microbiol Rev. 2006;19:449-490.
Atherton JC. The pathogenesis of Helicobacter pylori-induced gastro-duodenal diseases.Annu Rev Pathol. 2006;1:63-96.
Schistosomes , liver flukes and Helicobacter pylori. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Lyon, 7-14 June 1994.IARC Monogr Eval Carcinog Risks Hum. 1994;61:1-241.
Wen S, Moss SF. Helicobacter pylori virulence factors in gastric carcinogenesis.Cancer Lett. 2009;282:1-8.
Backert S, Ziska E, Brinkmann V, Zimny-Arndt U, Fauconnier A, Jungblut PR, Naumann M, Meyer TF. Translocation of the Helicobacter pylori CagA protein in gastric epithelial cells by a type IV secretion apparatus.Cell Microbiol. 2000;2:155-164.
Poppe M, Feller SM, Römer G, Wessler S. Phosphorylation of Helicobacter pylori CagA by c-Abl leads to cell motility.Oncogene. 2007;26:3462-3472.
Selbach M, Moese S, Hauck CR, Meyer TF, Backert S. Src is the kinase of the Helicobacter pylori CagA protein in vitro and in vivo.J Biol Chem. 2002;277:6775-6778.
Stein M, Bagnoli F, Halenbeck R, Rappuoli R, Fantl WJ, Covacci A. c-Src/Lyn kinases activate Helicobacter pylori CagA through tyrosine phosphorylation of the EPIYA motifs.Mol Microbiol. 2002;43:971-980.
Higashi H, Tsutsumi R, Muto S, Sugiyama T, Azuma T, Asaka M, Hatakeyama M. SHP-2 tyrosine phosphatase as an intracellular target of Helicobacter pylori CagA protein.Science. 2002;295:683-686.
Backert S, Selbach M. Role of type IV secretion in Helicobacter pylori pathogenesis.Cell Microbiol. 2008;10:1573-1581.
Segal ED, Cha J, Lo J, Falkow S, Tompkins LS. Altered states: involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori.Proc Natl Acad Sci USA. 1999;96:14559-14564.
Brandt S, Kwok T, Hartig R, König W, Backert S. NF-kappaB activation and potentiation of proinflammatory responses by the Helicobacter pylori CagA protein.Proc Natl Acad Sci USA. 2005;102:9300-9305.
Lamb A, Yang XD, Tsang YH, Li JD, Higashi H, Hatakeyama M, Peek RM, Blanke SR, Chen LF. Helicobacter pylori CagA activates NF-kappaB by targeting TAK1 for TRAF6-mediated Lys 63 ubiquitination.EMBO Rep. 2009;10:1242-1249.
Sharma SA, Tummuru MK, Blaser MJ, Kerr LD. Activation of IL-8 gene expression by Helicobacter pylori is regulated by transcription factor nuclear factor-kappa B in gastric epithelial cells.J Immunol. 1998;160:2401-2407.
Covacci A, Censini S, Bugnoli M, Petracca R, Burroni D, Macchia G, Massone A, Papini E, Xiang Z, Figura N. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer.Proc Natl Acad Sci USA. 1993;90:5791-5795.
Tummuru MK, Cover TL, Blaser MJ. Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production.Infect Immun. 1993;61:1799-1809.
Higashi H, Tsutsumi R, Fujita A, Yamazaki S, Asaka M, Azuma T, Hatakeyama M. Biological activity of the Helicobacter pylori virulence factor CagA is determined by variation in the tyrosine phosphorylation sites.Proc Natl Acad Sci USA. 2002;99:14428-14433.
Backert S, Tegtmeyer N, Selbach M. The versatility of Helicobacter pylori CagA effector protein functions: The master key hypothesis.Helicobacter. 2010;15:163-176.
Argent RH, Kidd M, Owen RJ, Thomas RJ, Limb MC, Atherton JC. Determinants and consequences of different levels of CagA phosphorylation for clinical isolates of Helicobacter pylori.Gastroenterology. 2004;127:514-523.
Azuma T, Yamakawa A, Yamazaki S, Fukuta K, Ohtani M, Ito Y, Dojo M, Yamazaki Y, Kuriyama M. Correlation between variation of the 3’ region of the cagA gene in Helicobacter pylori and disease outcome in Japan.J Infect Dis. 2002;186:1621-1630.
Quiroga AJ, Huertas A, Cómbita AL, Bravo MM. Variation in the number of EPIYA-C repeats in CagA protein from Colombian Helicobacter pylori strains and its ability middle to induce hummingbird phenotype in gastric epithelial cells.Biomedica. 2010;30:251-258.
Quiroga AJ, Huertas A, Combita AL, Bravo MM. Variation in the number of EPIYA-C repeats in CagA protein from Colombian Helicobacter pylori strains and its ability middle to induce hummingbird phenotype in gastric epithelial cells.Biomedica. 2010;30:251-258.
Ando T, Kusugami K, Ohsuga M, Shinoda M, Sakakibara M, Saito H, Fukatsu A, Ichiyama S, Ohta M. Interleukin-8 activity correlates with histological severity in Helicobacter pylori-associated antral gastritis.Am J Gastroenterol. 1996;91:1150-1156.
Peek RM, Miller GG, Tham KT, Perez-Perez GI, Zhao X, Atherton JC, Blaser MJ. Heightened inflammatory response and cytokine expression in vivo to cagA+ Helicobacter pylori strains.Lab Invest. 1995;73:760-770.
Argent RH, Hale JL, El-Omar EM, Atherton JC. Differences in Helicobacter pylori CagA tyrosine phosphorylation motif patterns between western and East Asian strains, and influences on interleukin-8 secretion.J Med Microbiol. 2008;57:1062-1067.
Naito M, Yamazaki T, Tsutsumi R, Higashi H, Onoe K, Yamazaki S, Azuma T, Hatakeyama M. Influence of EPIYA-repeat polymorphism on the phosphorylation-dependent biological activity of Helicobacter pylori CagA.Gastroenterology. 2006;130:1181-1190.
Schneider N, Krishna U, Romero-Gallo J, Israel DA, Piazuelo MB, Camargo MC, Sicinschi LA, Schneider BG, Correa P, Peek RM. Role of Helicobacter pylori CagA molecular variations in induction of host phenotypes with carcinogenic potential.J Infect Dis. 2009;199:1218-1221.
Acosta N, Quiroga A, Delgado P, Bravo MM, Jaramillo C. Helicobacter pylori CagA protein polymorphisms and their lack of association with pathogenesis.World J Gastroenterol. 2010;16:3936-3943.
Tummuru MK, Sharma SA, Blaser MJ. Helicobacter pylori picB, a homologue of the Bordetella pertussis toxin secretion protein, is required for induction of IL-8 in gastric epithelial cells.Mol Microbiol. 1995;18:867-876.
Maeda S, Yoshida H, Ikenoue T, Ogura K, Kanai F, Kato N, Shiratori Y, Omata M. Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates.Gut. 1999;44:336-341.
Bravo LE, van Doom LJ, Realpe JL, Correa P. Virulence-associated genotypes of Helicobacter pylori: do they explain the African enigma?Am J Gastroenterol. 2002;97:2839-2842.
Quiroga AJ, Cittelly DM, Bravo MM. [BabA2, oipA and cagE Helicobacter pylori genotypes in Colombian patients with gastroduodenal diseases].Biomedica. 2005;25:325-334.
Blaser MJ, Perez-Perez GI, Kleanthous H, Cover TL, Peek RM, Chyou PH, Stemmermann GN, Nomura A. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach.Cancer Res. 1995;55:2111-2115.
Kauser F, Khan AA, Hussain MA, Carroll IM, Ahmad N, Tiwari S, Shouche Y, Das B, Alam M, Ali SM. The cag pathogenicity island of Helicobacter pylori is disrupted in the majority of patient isolates from different human populations.J Clin Microbiol. 2004;42:5302-5308.
Jones KR, Joo YM, Jang S, Yoo YJ, Lee HS, Chung IS, Olsen CH, Whitmire JM, Merrell DS, Cha JH. Polymorphism in the CagA EPIYA motif impacts development of gastric cancer.J Clin Microbiol. 2009;47:959-968.
Nguyen LT, Uchida T, Tsukamoto Y, Trinh TD, Ta L, Mai HB, Le HS, Ho DQ, Hoang HH, Matsuhisa T. Clinical relevance of cagPAI intactness in Helicobacter pylori isolates from Vietnam.Eur J Clin Microbiol Infect Dis. 2010;29:651-660.
Backert S, Schwarz T, Miehlke S, Kirsch C, Sommer C, Kwok T, Gerhard M, Goebel UB, Lehn N, Koenig W. Functional analysis of the cag pathogenicity island in Helicobacter pylori isolates from patients with gastritis, peptic ulcer, and gastric cancer.Infect Immun. 2004;72:1043-1056.
Argent RH, Thomas RJ, Letley DP, Rittig MG, Hardie KR, Atherton JC. Functional association between the Helicobacter pylori virulence factors VacA and CagA.J Med Microbiol. 2008;57:145-150.
Viala J, Chaput C, Boneca IG, Cardona A, Girardin SE, Moran AP, Athman R, Mémet S, Huerre MR, Coyle AJ. Nod1 responds to peptidoglycan delivered by the Helicobacter pylori cag pathogenicity island.Nat Immunol. 2004;5:1166-1174.
Kaparakis M, Turnbull L, Carneiro L, Firth S, Coleman HA, Parkington HC, Le Bourhis L, Karrar A, Viala J, Mak J. Bacterial membrane vesicles deliver peptidoglycan to NOD1 in epithelial cells.Cell Microbiol. 2010;12:372-385.
Crabtree JE, Farmery SM, Lindley IJ, Figura N, Peichl P, Tompkins DS. CagA/cytotoxic strains of Helicobacter pylori and interleukin-8 in gastric epithelial cell lines.J Clin Pathol. 1994;47:945-950.
Owen RJ, Sharp S, Lawson AJ, Durrani Z, Rijpkema S, Kidd M. Investigation of the biological relevance of Helicobacter pylori cagE locus diversity, presence of CagA tyrosine phosphorylation motifs and vacuolating cytotoxin genotype on IL-8 induction in gastric epithelial cells.FEMS Immunol Med Microbiol. 2003;36:135-140.
Reyes-Leon A, Atherton JC, Argent RH, Puente JL, Torres J. Heterogeneity in the activity of Mexican Helicobacter pylori strains in gastric epithelial cells and its association with diversity in the cagA gene.Infect Immun. 2007;75:3445-3454.
Sgouras DN, Panayotopoulou EG, Papadakos K, Martinez-Gonzalez B, Roumbani A, Panayiotou J, vanVliet-Constantinidou C, Mentis AF, Roma-Giannikou E. CagA and VacA polymorphisms do not correlate with severity of histopathological lesions in Helicobacter pylori-infected Greek children.J Clin Microbiol. 2009;47:2426-2434.
Sicinschi LA, Correa P, Peek RM, Camargo MC, Piazuelo MB, Romero-Gallo J, Hobbs SS, Krishna U, Delgado A, Mera R. CagA C-terminal variations in Helicobacter pylori strains from Colombian patients with gastric precancerous lesions.Clin Microbiol Infect. 2010;16:369-378.
Strömberg E, Edebo A, Lundin BS, Bergin P, Brisslert M, Svennerholm AM, Lindholm C. Down-regulation of epithelial IL-8 responses in Helicobacter pylori-infected duodenal ulcer patients depends on host factors, rather than bacterial factors.Clin Exp Immunol. 2005;140:117-125.
Panayotopoulou EG, Sgouras DN, Papadakos KS, Petraki K, Breurec S, Michopoulos S, Mantzaris G, Papatheodoridis G, Mentis A, Archimandritis A. CagA and VacA polymorphisms are associated with distinct pathological features in Helicobacter pylori-infected adults with peptic ulcer and non-peptic ulcer disease.J Clin Microbiol. 2010;48:2237-2239.
Ren S, Higashi H, Lu H, Azuma T, Hatakeyama M. Structural basis and functional consequence of Helicobacter pylori CagA multimerization in cells.J Biol Chem. 2006;281:32344-32352.
Lu HS, Saito Y, Umeda M, Murata-Kamiya N, Zhang HM, Higashi H, Hatakeyama M. Structural and functional diversity in the PAR1b/MARK2-binding region of Helicobacter pylori CagA.Cancer Sci. 2008;99:2004-2011.
Torres J, Correa P, Ferreccio C, Hernandez-Suarez G, Herrero R, Cavazza-Porro M, Dominguez R, Morgan D. Gastric cancer incidence and mortality is associated with altitude in the mountainous regions of Pacific Latin America.Cancer Causes Control. 2013;24:249-256.