CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses

Figure 3 CagA-protein phosphorylation and its relationship to Glu-Pro-Ile-Tyr-Ala-C (EPIYA-C) motifs and disease severity. Sixty functional-cagPAI strains were cocultured with AGS cells for 6 h. Coculture lysates were assessed by Western blot and levels of CagA phosphorylation were determined by densitometry. A: Evaluation of CagA phosphorylation levels according to the pathology from which strains were isolated; B: Relationship between the number of EPIYA motifs and the levels of CagA phosphorylation. G: Gastritis; DU: Duodenal ulcer; AG: Atrophic gastritis; IM: Intestinal metaplasia; GC: Gastric cancer. NS: Not significant