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©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. May 15, 2025; 17(5): 102417
Published online May 15, 2025. doi: 10.4251/wjgo.v17.i5.102417
Published online May 15, 2025. doi: 10.4251/wjgo.v17.i5.102417
Long noncoding RNA SNHG5 promotes 5-fluorouracil resistance in colorectal cancer by regulating miR-26b/p-glycoprotein axis
Bin Wang, Ya-Dong Feng, Li-Hua Ren, Rui-Hua Shi, School of Medicine, Southeast University, Nanjing 210009, Jiangsu Province, China
Bin Wang, Qian Zhou, Cui-E Cheng, Yi-Jie Gu, Department of Gastroenterology, The Affiliated Changshu Hospital of Nantong University, Changshu No. 2 People’s Hospital, Suzhou 215500, Jiangsu Province, China
Ting-Wang Jiang, Department of Key Laboratory, The Affiliated Changshu Hospital of Nantong University, Changshu No. 2 People’s Hospital, Suzhou 215500, Jiangsu Province, China
Jia-Ming Qiu, Department of Pathology, The Affiliated Changshu Hospital of Nantong University, Changshu No. 2 People’s Hospital, Suzhou 215500, Jiangsu Province, China
Gui-Ning Wei, Department of Pharmacology, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning 530022, Guangxi Zhuang Autonomous Region, China
Ya-Dong Feng, Li-Hua Ren, Rui-Hua Shi, Department of Gastroenterology, Zhongda Hospital, Southeast University, Nanjing 210009, Jiangsu Province, China
Author contributions: Wang B contributed to the conceptualization of the study, methodology, investigation, formal analysis, and writing and editing of the manuscript; Zhou Q, Gu YJ and Qiu JM contributed to the formal analysis and data curation; Cheng CE contributed to the conceptualization of the study and supervision; Jiang TW, Wei GN and Ren LH contributed to the methodology and validation; Feng YD contributed to the investigation and formal analysis; Shi RH contributed to the conceptualization of the study, supervision, writing, review and editing; All authors have read and approved the final manuscript.
Supported by the National Natural Science Foundation of China, No. 82404088; China Postdoctoral Science Foundation, No. 2023M730587; and Changshu Talent Scientific Project, No. KCH202304.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board Changshu No. 2 People’s Hospital (No. 2020-KY-008).
Institutional animal care and use committee statement: The animal experiments were approved by the Animal Care and Welfare Committee of Southeast University (No. 20200721007).
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: The data generated or analyzed during this study are available from the corresponding author upon reasonable request.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Rui-Hua Shi, MD, Professor, School of Medicine, Southeast University, No. 87 Dingjiaqiao Road, Gulou District, Nanjing 210009, Jiangsu Province, China.
Received: October 18, 2024
Revised: February 3, 2025
Accepted: February 27, 2025
Published online: May 15, 2025
Processing time: 209 Days and 15.4 Hours
Revised: February 3, 2025
Accepted: February 27, 2025
Published online: May 15, 2025
Processing time: 209 Days and 15.4 Hours
Core Tip
Core Tip: This study confirmed that the expression of the long noncoding RNA SNHG5 was upregulated in 5-fluorouracil (5-Fu)-resistant colorectal cancer (CRC) tissues and 5-Fu-resistant CRC cells. Functional experiments confirmed that SNHG5 overexpression reduced 5-Fu sensitivity in CRC in vitro and in vivo. Mechanistically, SNHG5 acted as a molecular sponge for miR-26b. In addition, SNHG5 conferred 5-Fu resistance in CRC by regulating the miR-26b/p-glycoprotein axis. Our results elucidate potential mechanisms of noncoding RNAs in CRC chemoresistance and provide new strategies for CRC chemotherapy.