Clinical and Translational Research
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Jan 15, 2024; 16(1): 30-50
Published online Jan 15, 2024. doi: 10.4251/wjgo.v16.i1.30
Mechanism of pachymic acid in the treatment of gastric cancer based on network pharmacology and experimental verification
Yu-Hua Du, Jian-Jun Zhao, Xia Li, Shi-Cong Huang, Na Ning, Guo-Qing Chen, Yi Yang, Yi Nan, Ling Yuan
Yu-Hua Du, Jian-Jun Zhao, Xia Li, Shi-Cong Huang, Na Ning, Guo-Qing Chen, Ling Yuan, College of Pharmacy, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Xia Li, Ningxia Chinese Medicine Research Center, Manufacturing Laboratory, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Yi Yang, College of Foundation, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Yi Nan, Key Laboratory of Ningxia Minority Medicine Modernization Ministry of Education, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Co-corresponding authors: Yi Nan and Ling Yuan.
Author contributions: Du YH carried out most of the studies, analyzed the data and wrote the manuscript; Nan Y designed the study and revised the manuscript; Huang SC, Ning N, and Chen GQ wrote the manuscript and carried out the chart-making work; Li X and Yang Y performed parts of the experiments and conducted statistical analyses of the data; Yuan L supervised the process of research and provided clinical guidance; Yuan L and Zhao JJ provided the conceptual and technical guidance as well as revised the manuscript critically for important intellectual content; and all authors have read and approved the manuscript. Yuan L and Nan Y have guided this research throughout the whole process, provided technical and theoretical support for this research, and made great contributions.
Supported by Ningxia Science and Technology Benefiting People Program, No. 2022CMG03064; National Natural Science Foundation of China, No. 82260879; Ningxia Natural Science Foundation, No. 2022AAC03144 and 2022AAC02039.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board of Ningxia Medical University (No. 2023-002).
Clinical trial registration statement: Our research does not involve clinical trial.
Informed consent statement: All of our experiments are designed and performed only in the laboratory. We didn’t test clinical sample data, no informed consent is required.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: All data generated or analyzed during this study are included in this paper, and further inquiries can be directed to the corresponding author (
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Ling Yuan, PhD, Professor, College of Pharmacy, Ningxia Medical University, No. 1160 Shengli Street, Yinchuan 750004, Ningxia Hui Autonomous Region, China.
Received: July 29, 2023
Peer-review started: July 29, 2023
First decision: September 25, 2023
Revised: October 7, 2023
Accepted: November 2, 2023
Article in press: November 2, 2023
Published online: January 15, 2024
Core Tip

Core Tip: Pachymic acid (PA) is an important bioactive component of Poria cocos. Network pharmacology analysis showed that the core targets of PA in treating gastric cancer (GC) were proto-oncogene tyrosine-protein kinase Src, mitogen-activated protein kinase 1, phosphatidylinositol 3-kinase regulatory subunit alpha, heat shock protein 90-alpha, and tyrosine-protein phosphatase non-receptor type 11. Molecular docking results showed that PA could combine well with the core targets. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and gene set enrichment analysis suggested that the PI3K/AKT signaling pathway was possible for treating GC with PA. The experiment results demonstrated that PA could decrease the survival rate of HGC-27 cells, halt the progression of the cell cycle, enhance cell apoptosis, control the PI3K/AKT signaling pathway to stimulate apoptosis, and impede cell growth.