Observational Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Sep 15, 2020; 12(9): 1056-1064
Published online Sep 15, 2020. doi: 10.4251/wjgo.v12.i9.1056
Methylation changes at the GNAS imprinted locus in pancreatic cystic neoplasms are important for the diagnosis of malignant cysts
Sandra Faias, Marlene Duarte, Luísa Pereira, Paula Chaves, Marília Cravo, Antonio Dias Pereira, Cristina Albuquerque
Sandra Faias, Antonio Dias Pereira, Department of Gastroenterology, Instituto Português de Oncologia de Lisboa Francisco Gentil, EPE, Lisboa 1099-023, Portugal
Sandra Faias, Paula Chaves, Faculty of Health Sciences, University of Beira Interior, Covilhã 6200-506, Portugal
Marlene Duarte, Cristina Albuquerque, Unidade de Investigação em Patobiologia Molecular (UIPM), Instituto Português de Oncologia de Lisboa Francisco Gentil, EPE, Lisboa 1099-023, Portugal
Luísa Pereira, Centro de Matemática e Aplicações (CMA-UBI), Universidade da Beira Interior, Covilhã 6200-506, Portugal
Paula Chaves, Department of Pathology, Instituto Português de Oncologia de Lisboa Francisco Gentil, EPE, Lisboa 1099-023, Portugal
Marília Cravo, Department of Gastroenterology, Hospital Beatriz Ângelo, Loures 2674-514, Portugal
Author contributions: All authors have contributed to the paper concept and design and agreed on the final content of the manuscript; Faias S, Duarte M, and Albuquerque C acquired and interpreted the data; Pereira L performed the statistical analysis; Faias S, Pereira L, Chaves P, Cravo M, and Albuquerque C drafted the manuscript under the supervision of both senior authors, Albuquerque C and Pereira AD; all authors critically revised the manuscript and approved the final version of the manuscript.
Supported by a Research Grant from Sociedade Portuguesa de Endoscopia Digestiva in 2018.
Institutional review board statement: The study was approved by the Ethics Committee and Institutional Scientific Board (UIC/1143).
Informed consent statement: All patients gave informed consent.
Conflict-of-interest statement: The authors have no conflicts of interest to disclose.
Data sharing statement: No additional data are available.
STROBE statement: Guidelines of STROBE statement have been adopted.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Sandra Faias, MD, Attending Doctor, Research Fellow, Department of Gastroenterology, Instituto Português de Oncologia de Lisboa Francisco Gentil, EPE, Rua Prof Lima Basto, Lisboa 1099-023, Portugal. sandrarfaias@hotmail.com
Received: April 15, 2020
Peer-review started: April 15, 2020
First decision: July 5, 2020
Revised: July 18, 2020
Accepted: August 1, 2020
Article in press: August 1, 2020
Published online: September 15, 2020
Core Tip

Core Tip: Pancreatic cystic lesions are a clinical dilemma due to risk of malignancy. Somatic mutations of guanine nucleotide-binding protein, alpha stimulating (GNAS) are characteristic of intraductal papillary mucinous neoplasms. We found methylation changes in differential methylated regions at the GNAS locus in pancreatic cyst fluid predominantly of malignant cysts. Methylation changes in GNAS locus may improve the diagnosis of malignant cysts and shed light on the development of novel therapeutic approaches for pancreatic cancer.