Retrospective Study
Copyright ©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. May 15, 2025; 17(5): 105872
Published online May 15, 2025. doi: 10.4251/wjgo.v17.i5.105872
Integrating ultrasound and serum indicators for evaluating outcomes of targeted immunotherapy in advanced liver cancer
Hai-Bin Tu, Si-Yi Feng, Li-Hong Chen, Yu-Jie Huang, Ju-Zhen Zhang, Su-Yu Peng, Ding-Luan Lin, Xiao-Jian Ye
Hai-Bin Tu, Si-Yi Feng, Li-Hong Chen, Yu-Jie Huang, Ju-Zhen Zhang, Su-Yu Peng, Department of Ultrasound, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, Fujian Province, China
Ding-Luan Lin, Department of Positron Emission Tomography, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, Fujian Province, China
Xiao-Jian Ye, Department of Ultrasound, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China
Xiao-Jian Ye, Department of Ultrasound, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China
Co-first authors: Hai-Bin Tu and Si-Yi Feng.
Author contributions: Tu HB contributed to conceptualization and wrote the paper; Feng SY performed the data statistical analysis; Tu HB and Feng SY contributed equally to this work as co-first authors; Chen LH, Huang YJ, Zhang JZ, Peng SY, and Lin DL followed the patients; Ye XJ revised the manuscript and gave guide for the study; and all authors read and approved the final manuscript.
Supported by Natural Science Foundation of Fujian Province, No. 2022J011285 and No. 2023J011480; Fuzhou Municipal Bureau of Science and Technology Program Fund, No. 2021-S-109; Provincial Subsidy Fund for Health and Wellness from Fujian Provincial Department of Finance, No. BPB-2022YXJ; and Fujian Provincial Health Technology Project, No. 2020GGB032.
Institutional review board statement: This study was reviewed and approved by Mengchao Hepatobiliary Hospital Ethics Committee (Approval No. 2021_084_01).
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The datasets generated and/or analyzed during the current study are not publicly available due to being generated based on information collected during clinical care but are available in de-identified form from the corresponding author(xjian_y@163.com) on reasonable request at the study’s close.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Xiao-Jian Ye, Associate Professor, Department of Ultrasound, The First Affiliated Hospital of Fujian Medical University, No. 20 Chazhong Road, Taijiang District, Fuzhou 350001, Fujian Province, China. xjian_y@163.com
Received: February 10, 2025
Revised: March 27, 2025
Accepted: April 17, 2025
Published online: May 15, 2025
Processing time: 96 Days and 0.1 Hours
Abstract
BACKGROUND

Hepatocellular carcinoma (HCC) is a major global contributor to cancer-related mortality, with advanced stages presenting substantial therapeutic challenges. Although targeted immunotherapy shows potential, many patients exhibit poor responses, underscoring the need for predictive tools to optimize treatment strategies. Emerging data indicate that ultrasound features (e.g., tumor stiffness) and serum biomarkers may serve as predictors of treatment outcomes. However, an integrated model for these predictors remains unavailable. This paper introduces a machine learning-based approach that combines ultrasound and serological data to forecast immunotherapy efficacy in patients with advanced HCC.

AIM

To develop a non-invasive predictive model for targeted immunotherapy in advanced HCC, incorporating both internal and external validation.

METHODS

Patients with advanced HCC who received targeted immunotherapy at two medical centers were enrolled and divided into internal training, internal validation, and external validation cohorts. Comprehensive clinical data were gathered. Initially, 13 machine learning algorithms were tested using the internal training cohort. The algorithm yielding the highest area under the curve (AUC) in the internal validation cohort was selected to construct a predictive model, termed the Target Immunotherapy Predictive Model (TIPM). TIPM performance was then compared with that of traditional tumor staging systems (tumor-node-metastasis, Barcelona Clinic Liver Cancer, China Liver Cancer, Hong Kong Liver Cancer, and C-reactive protein and alpha-fetoprotein in immunotherapy).

RESULTS

A total of 306 patients participated in the study, with 143 in the internal training cohort, 62 in the internal validation cohort, and 101 in the external validation cohort. In the internal validation cohort, the random forest model achieved the highest AUC (0.975, 95% confidence interval: 0.924-0.998). The key predictors for TIPM were tumor size, platelet count, tumor stiffness change, and white blood cell count. During external validation, TIPM outperformed conventional models, reaching an AUC of 0.899 (95% confidence interval: 0.840-0.957). Calibration curves demonstrated strong concordance with observed outcomes, while decision curve analysis confirmed TIPM’s enhanced clinical value. Additional metrics, such as the net reclassification index and integrated discrimination improvement, further supported TIPM’s superior predictive accuracy.

CONCLUSION

TIPM provides a robust tool for predicting targeted immunotherapy efficacy in advanced HCC, facilitating personalized treatment planning.

Keywords: Advanced hepatocellular carcinoma; Targeted immunotherapy; Predictive modeling; Non-invasive; Machine learning

Core Tip: Predicting treatment response in patients with advanced hepatocellular carcinoma undergoing targeted immunotherapy remains a significant challenge. This study developed and validated the Target Immunotherapy Predictive Model, a novel, non-invasive tool that integrates pre-treatment ultrasound features (tumor diameter, changes in tumor stiffness) and accessible serum biomarkers (platelet count, white blood cell count) using machine learning. The Target Immunotherapy Predictive Model outperformed traditional staging systems in predicting treatment efficacy, providing a promising solution for personalized treatment selection in advanced hepatocellular carcinoma. This approach has the potential to minimize unnecessary treatments, optimize therapeutic strategies, and ultimately improve patient outcomes. A user-friendly web calculator further enhances its clinical applicability.