Retrospective Cohort Study Open Access
Copyright ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Hepatol. Feb 27, 2018; 10(2): 308-318
Published online Feb 27, 2018. doi: 10.4254/wjh.v10.i2.308
Survival outcomes of liver transplantation for hepatocellular carcinoma in patients with normal, high and very high preoperative alpha-fetoprotein levels
Wong Hoi She, Department of Surgery, University of Hong Kong, Hong Kong, China
Albert Chi Yan Chan, Tan To Cheung, Chung Mau Lo, Kenneth Siu Ho Chok, Department of Surgery and State Key Laboratory for Liver Research, University of Hong Kong, Hong Kong, China
ORCID number: Wong Hoi She (0000-0003-2049-3140); Albert Chi Yan Chan (0000-0002-1383-2952); Tan To Cheung (0000-0002-2633-5883); Chung Mau Lo (0000-0002-3964-5995); Kenneth Siu Ho Chok (0000-0001-7921-3807).
Author contributions: She WH contributed to study conception and design, data acquisition, analysis, interpretation and manuscript drafting; Chan ACY, Cheung TT and Lo CM contributed to critical revision of the manuscript; Chok KSH contributed to study conception and design, drafting and revision of the manuscript; all authors approved the submitted version of the paper.
Institutional review board statement: Institutional review board approval was not required for this retrospective study since treatments given to patients were not influenced by the study and the clinical data used in the study are anonymous.
Informed consent statement: Patients’ consent to this retrospective study was not required since treatments given to patients were not influenced by the study and no individual patients would be identified as the clinical data used in the study are anonymous.
Conflict-of-interest statement: None of the authors has any conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Kenneth Siu Ho Chok, FRCS (Ed), Associate Professor, Department of Surgery, University of Hong Kong, 102 Pok Fu Lam Road, Hong Kong, China. chok6275@hku.hk
Telephone: +852-22553025 Fax: +852-28165284
Received: November 23, 2017
Peer-review started: November 24, 2017
First decision: December 12, 2017
Revised: December 18, 2017
Accepted: February 5, 2018
Article in press: February 5, 2018
Published online: February 27, 2018

Abstract
AIM

To investigate the impact of alpha-fetoprotein (AFP) on long-term recurrence rate and overall survival and we also aimed to define the level of AFP leading to a higher risk of disease recurrence and affecting patient survival.

METHODS

Data of adult patients who received liver transplant (LT) for hepatocellular carcinoma (HCC) at our hospital from January 2000 to December 2013 were reviewed. Reviewed data included demographic characteristics, preoperative AFP level, operative details, follow-up details, and survival outcomes. Patients were mostly listed for LT based on Milan or UCSF criteria. For the purpose of this study, normal AFP level was defined as AFP value < 10 ng/mL, high AFP level was defined as AFP value ≥ 10 to < 400 ng/mL, and very high AFP level was defined as AFP ≥ 400 ng/mL. The patients were divided into these 3 groups accordingly. Survival rates were plotted as Kaplan-Meier curves and compared by log-rank analysis. Continuous variables were expressed as median (interquartile range). Categorical variables were compared by Spearman’s test. Discriminative analysis was used to define the lowest value of AFP that could affect the overall survival in study population. Statistical significance was defined by a P value of < 0.05.

RESULTS

Totally 250 adult patients underwent LT for HCC in the study period. Eight-four of them received deceased-donor LT and 166 had living-donor LT. The patients were divided into 3 groups: Group A, AFP < 10 ng/mL (n = 83); Group B, AFP ≥ 10 to < 400 ng/mL (n = 131); Group C, AFP ≥ 400 ng/mL (n = 36). The commonest etiology was hepatitis-B-related cirrhosis. The Model for End-stage Liver Disease scores in these groups were similar (median, 13 vs 13 vs 12; P = 0.745). The time to operation in Group A was longer (median, 94 vs 31 vs 35 d; P = 0.001). The groups were similar in hospital mortality (P = 0.626) and postoperative complication (P = 0.702). Pathology of explants showed that the 3 groups had similar numbers of tumor nodules, but the tumors in Group C were larger (A: 2.5 cm, B: 3.0 cm, C: 4.0 cm; P = 0.003). Group C had a bigger proportion of patients who were beyond Milan criteria (P = 0.010). Poor differentiation and vascular permeation were also more common in this group (P = 0.017 and P = 0.003 respectively). It also had poorer 5-year survival (A: 85.5%, B: 82.4%, C: 66%; P = 0.029). The 5-year disease-free survival was 84.3% in Group A, 80.1% in Group B, and 61.1% in Group C. Receiver operating characteristic area under the curve for AFP in predicting tumor recurrence was 0.685. The selected cut-off value was 54 ng/mL for AFP (C-index 0.685; 95%CI: 0.592-0.779; sensitivity 0.595; specificity 0.687). On discriminative analysis, AFP value of 105 ng/mL was shown to affect the overall survival of the patients.

CONCLUSION

HCC patients with a high preoperative AFP level had inferior survival after LT. AFP level of 54 ng/mL was associated with disease recurrence, and AFP level of 105 ng/mL was found to be the cut-off value for overall survival difference.

Key Words: Alpha-fetoprotein, Liver transplantation, Recurrence, Survival

Core tip: Various established criteria have been used to identify patients with hepatocellular carcinoma who would benefit from liver transplant with reasonable survival. Alpha-fetoprotein (AFP) level has been identified as an important factor associated with suboptimal survival with high recurrence rate. This study demonstrated that AFP level correlated well with the pathological findings of tumor differentiation and microvascular invasion, which are usually confirmed in explant pathology. In this set of data, AFP level of 54 ng/mL was associated with disease recurrence, and AFP level of 105 ng/mL was found to be the cut-off value for overall survival difference.



INTRODUCTION

Liver transplant (LT) is the best treatment option for hepatocellular carcinoma (HCC) as it removes both the tumor and the cirrhotic liver. The Milan criteria have been well adopted worldwide as a set of guidelines for listing patients for LT. Patients within the Milan criteria have a 5-year post-LT survival of 65%-80%, with a recurrence risk of 8%-15%[1]. However, the Milan criteria are criticized for being too stringent, since many patients beyond the criteria could still have reasonable post-LT survival[2-8]. Therefore, in additional to morphological consideration of tumor, the adoption of biological markers such as alpha-fetoprotein (AFP), response to therapy and evolution after therapy[9] is advocated.

AFP has been used as a tumor marker for HCC, and a high AFP level has been shown to be associated with poorer outcomes[10,11]. In previous studies, suboptimal results with high recurrence rates were seen in patients who had received LT with an AFP level of > 1000 ng/mL[12-14]. Such a level is considered a contraindication to LT. This level is applied not only to extended criteria but also to patients within the Milan criteria. Unfortunately, the exact consensual cut-off value remains undefined.

In this study, we investigated the impact of AFP on long-term recurrence rate and overall survival. We also aimed to define the level of AFP leading to a higher risk of disease recurrence and affecting patient survival.

MATERIALS AND METHODS

Prospectively collected data of adult patients who received deceased-donor LT (DDLT) or living-donor LT (LDLT) for HCC at our hospital in the period from January 2000 to December 2013 were reviewed and analyzed. These data included demographic characteristics, preoperative AFP level, operative details, follow-up details, and survival outcomes. Institutional review board approval was not required for this study because it was a retrospective analysis of anonymous data. Patient treatments were not affected by this study.

Patient selection for LT

The strategies adopted for selection of patients with known HCC for LT have been described elsewhere[15,16]. In brief, tumor evaluation was done with computed tomography of the abdomen and thorax, in addition to radionuclide bone scan at initial diagnosis. In recent years, dual-tracer (11C-acetate and 18 F-fluorodeoxyglucose) positron emission tomography (PET) was performed to exclude extrahepatic metastasis. Patients who were 65 years old or younger and not eligible for partial hepatectomy or local ablation were considered for LT. The age limit as a selection criterion was getting relatively loose as long as the patient was physically fit. The Milan criteria[1] and the UCSF criteria[14] were used for selection of patients for listing. Patients who had recurrent HCC after hepatectomy would still be considered for LT if their disease was still within selection criteria. There was no mandatory waiting period prior to LT, and bridging therapy with transarterial chemoembolization was offered to LT candidates with reasonable liver function. From October 2009 onwards, an arbitrary Model for end-stage liver disease (MELD) score of 18 points were given to DDLT candidates with HCC remaining at stage 2 six months after radiological confirmation of their stage-2 disease. Two MELD points were added every three months as long as their disease remained at stage 2 or below[17].

Patients who were beyond the Milan and UCSF criteria because they had slightly larger tumors or slightly more tumors were not eligible for DDLT but could be considered for LDLT if they had no portal or hepatic vein invasion.

Treatments

Surgery was performed using standard techniques. Cell-saver device was not used. Explants were examined by pathologists for tumor size and number, differentiation, and presence of microscopic vascular invasion. Tumors found on explant examination were regarded as incidental tumors. Neither medical nor radiation adjuvant treatment was given to any patient after LT. The patients were monitored regularly by measurement of serum AFP level, chest radiography and abdominal and chest computed tomography every 3 mo. Recurrences suspected on clinical grounds were confirmed by histological examination as far as possible.

Donor and recipient operations were performed as described elsewhere[18]. The decision to use left-lobe graft vs right-lobe graft was based on a number of donor and recipient factors, the most important of which were the ratio of graft weight to standard liver volume, the ratio of graft weight to recipient weight, MELD score, and donor liver anatomy. The Urata formula [Liver volume (mL) = Body surface area (m2) × 706.2 + 2.4] was used to calculate standard liver volume[19]. Implantation process and techniques were similar for left and right lobe grafts[18,20]. The immunosuppression and prophylaxis regimens prescribed have been described earlier[21].

Statistical analysis

For the purpose of this study, normal AFP level was defined as AFP value < 10 ng/mL, high AFP level was defined as AFP value ≥ 10 to < 400 ng/mL, and very high AFP level was defined as AFP ≥ 400 ng/mL. The patients were divided into these 3 groups accordingly.

Receiver operating characteristic (ROC) analysis was used to evaluate the ability of AFP to predict postoperative recurrence and to choose the optimal cut-off value for subsequent analysis. For indication for LDLT, high specificity was essential for avoiding excluding a large number of patients who would not develop recurrence.

Clinical profiles and outcomes of patients were compared on the basis of AFP level. Comparisons were made on short- and long-term outcomes, including graft function, graft survival, patient survival, and incidence of biliary complication. Continuous variables were expressed as median (range), and the Mann-Whitney U test was used for subgroup comparison. Categorical variables were compared by χ2 test or Fisher’s exact test. The cumulative probability of recurrence and survival was estimated by the life-table method and compared by the log-rank test. Deaths from all causes were included in the calculation of survival. Patients without recurrence were regarded as censored observations in the calculation of cumulative recurrence rates. Variables related to graft, tumor and tumor treatment before LT were analyzed for prognostic significance. The Kaplan-Meier method was used for survival analysis and the log-rank test was used for survival comparison. Discriminative analysis was used to define the lowest value of AFP that could affect the overall survival in the study population. Statistical significance was defined by a P value of < 0.05. The computer software SPSS, version 20.0 (SPSS Inc., Chicago, IL, United States), was used for all statistical calculations.

RESULTS

From January 2000 to December 2013, 250 adult patients underwent LT for HCC. Eight-four of them received DDLT and 166 had LDLT. The patients were divided into 3 main groups according to their preoperative AFP level: Group A, AFP < 10 ng/mL (normal); Group B, AFP ≥ 10 to < 400 ng/mL (high); Group C, AFP ≥ 400 ng/mL (very high) (Table 1). Patients in Group C were significantly younger (P = 0.037). The 3 groups has similar distribution of sex (P = 0.492). The commonest etiology was hepatitis-B-related cirrhosis. The median MELD scores in the 3 groups were similar (P = 0.745). The median time to operation in Group A was significantly longer (P = 0.001).

Table 1 Comparison of Group A, Group B and Group C.
Group A (n = 83)Group B (n = 131)Group C (n = 36)P value
Age (yr)56 (38-65)55 (3-72)51.5 (11-66)0.037
Male/Female67/16113/1829/70.492
Diagnosis:
Cirrhosis
Cryptogenic221
Hepatitis B678925
Hepatitis C4223
Alcoholic011
Hepatitis B + C120
Alcoholic + hepatitis C101
Alcoholic + hepatitis B010
Autoimmune100
Wilson's disease000
Preoperative MELD score13 (6-35)12 (6-35)12 (8-43)0.745
Waiting time (d)94 (1-2735)31 ( 1-1874)35 (1-1473)0.001
Blood transfusion (units)4.2 (0-32)2 (0-56)4 (0-31)0.128
Fresh frozen plasma transfusion (units)8 (0-24)6 (0-30)6 (0-22)0.609
Platelet transfusion (units)8 (0-26)6 (0-32)8 (0-22)0.978
Operation time (min)650 (370-1105)678 (333-1110)707 (300-1273)0.598
Cold ischemic time (min)182 (62-652)125 (60-633)133 (70-500)0.206
Warm ischemic time (min)49.5 (25-102)52 (26-108)55.5 (30-93)0.209
Hospital stay (d)1.7 (8-132)15 (0-83)15 (7-47)0.251
Intensive care unit stay (d)3 (1-42)3 (0-30)3 (2-16)0.283
Follow-up (mo)82.4 (0.59-204.9)89.1 (0-210.82)68.2 (5.95-204.24)0.242
Hospital mortality2 (2.4%)2 (1.5%)00.626
LDLT:DDLT45:3893:3828:80.012
Explant Milan Within:Beyond56:2384:4615:210.010
Explant UCSF Within:Beyond63:1698:3223:130.188
No. of tumor in explant1 (1-multiple)2 (1-multiple)1 (1-20)0.272
Largest size of tumor in explant (cm)2.5 (0.90-7.00)3.0 (0.25-9.00)4.0 (1.5-19.5)0.003
Differentiation:0.017
Well26413
Moderate416625
Poor286
Undifferentiated020
Unknown10132
Vascular permeation:0.003
No608514
Yes184021
Unknown151
Graft loss18 (21.7%)28 (21.4%)15 (41.7%)0.033
Patient status Alive:Dead65:18104:2721:150.027
Graft survival, yr0.038
196.40%93.10%97.20%
389.20%84.70%80.60%
585.50%81.60%66.00%
Patient survival, yr0.029
196.40%94.70%97.20%
389.20%85.50%80.60%
585.50%82.40%66.00%
Disease-free survival, yr0.007
192.80%89.30%80.60%
388.00%81.70%72.20%
584.30%80.10%61.10%
Postoperative early complication by Clavien grading:0.702
No406820
I19249
II5132
IIIA11113
IIIB662
IVA170
IVB000
V120

There were no differences in terms of blood transfusion amount, operation time, cold ischemic time or warm ischemic time among the groups, suggesting that the operative procedures were similar in the groups. Moreover, no differences were found in intensive care unit stay (P = 0.283), hospital stay (P = 0.251), hospital mortality (P = 0.626), or postoperative complication (P = 0.702). Pathology of explants showed that the 3 groups had similar numbers of tumor nodules but the tumors in Group C were larger (P = 0.003), and thus more patients in Group C were beyond the Milan criteria (P = 0.010). Furthermore, Group C had more cases of poor differentiation (P = 0.017) and vascular permeation (P = 0.003).

No patients were lost to follow-up in the study period. The 3 groups had similar follow-up period (P = 0.242). More patients in Group C had graft loss (P = 0.033), and hence this group had poorer 5-year graft survival (P = 0.038) and 5-year patient survival (P = 0.029) (Figure 1). Most patients died of recurrent HCC. The 5 year-disease-free survival was 84.3% in Group A, 80.1% in Group B, and 61.1% in Group C. Disease-free survival was similar in Groups A and B (P = 0.813) but significantly different between Groups A and C (P = 0.004) and between Groups B and C (P = 0.006) (Figure 2).

Figure 1
Figure 1 Overall survival of patients with preoperative AFP < 10 ng/mL, ≥ 10 to < 400 ng/mL, and ≥ 400 ng/mL. AFP: Alpha-fetoprotein. bP < 0.01.
Figure 2
Figure 2 Disease-free survival of patients with preoperative AFP < 10 ng/mL, ≥ 10 to < 400 ng/mL, and ≥ 400 ng/mL. AFP: Alpha-fetoprotein. bP < 0.01.
Disease recurrence and ROC curve analysis

Recurrence of HCC was identified in 42 patients (42/250 = 16.8%). The ability of preoperative AFP to predict HCC recurrence was analyzed by ROC curve. Area under the curve for AFP was 0.685. Among the cut-off values with sufficient specificity, the cut-off point with the highest C-index was chosen as the optimal cut-off value for subsequent analysis. The selected cut-off value was 54 ng/mL for AFP (C-index 0.685; 95% confidence interval 0.592-0.779; sensitivity 0.595; specificity 0.687) (Figure 3).

Figure 3
Figure 3 Receiver operating characteristic curve for alpha-fetoprotein in predicting hepatocellular carcinoma recurrence after liver transplant.

Further analysis was performed to identify the lowest AFP level that could affect patient survival. On discriminative analysis, AFP value of 105 ng/mL was identified as the level that could affect the overall survival of the patients. Patients with AFP ≤ 105 ng/mL (Group D) were compared with patients with AFP >105 ng/mL (Group E) (Table 2). Patients in Group E were younger (P = 0.017). When it comes to preoperative comorbidity, underlying cause of cirrhosis, MELD score, operative details, postoperative complication and hospital stay, no significant differences were seen. However, Group E had poorer 5-year graft survival (P = 0.024), patient survival (P = 0.045) (Figure 4), and disease-free survival (P = 0.006) (Figure 5). Looking into the details of the pathological results of the 2 groups, it was clear that the tumors in Group E had worse pathology. Group E had larger tumors (P = 0.017) and fewer cases of well differentiation (15.71% vs 32.78%; P = 0.001), while vascular permeation was more common in this group (44.29% vs 26.67%; P = 0.014) (Table 2).

Table 2 Comparison of the two subgroups, Group D and Group E.
Group D (n = 180)Group E (n = 70)P value
Age (yr)56 (38-67)53.5 (3-72)0.017
Male:Female150:3059:110.855
Diagnosis:
Cirrhosis
Cryptogenic41
Hepatitis B13645
Hepatitis C1910
Alcoholic11
Hepatitis B + C12
Alcoholic + hepatitis C11
Alcoholic + hepatitis B10
Autoimmune10
Wilson's disease00
Preoperative MELD score12 (6-35)12 (6-43)0.972
Waiting time (d)58.5 (1-2735)32 (1-1874)0.183
Blood transfusion (units)3 (0-56)4(0-32)0.988
Fresh frozen plasma transfusion (units)6 (0-30)6 (0-22)0.798
Platelet transfusion (units)6 (0-30)8 (0-32)0.708
Operation time (min)654 (333-1110)716.5 (300-1273)0.151
Cold ischemic time (min)137.5 (60-652)127.5 (66-633)0.195
Warm ischemic time (min)51 (25-108)53 (28-93)0.308
Hospital stay (d)15.5 (0-132)16 (7-48)0.497
Intensive care unit stay (d)3 (0-42)3 (2-30)0.806
Follow-up (mo)87.2 (0.0-210.8)75.4 (3.8-206.8)0.173
Hospital mortality4 (2.2%)00.486
LDLT: DDLT112:6854:160.025
Explant Milan Within:Beyond116:6039:300.170
Explant UCSF Within:Beyond135:4149:200.354
No. of tumor in explant1.5 (1-multiple)1 (1-20)0.551
Largest size of tumor in explant (cm)2.85 (0.90-7.00)3.5 (0.25-19.5)0.017
Differentiation0.0001
Well5911
Moderate9141
Poor610
Undifferentiated02
Unknown205
Vascular permeation0.014
No12435
Yes4831
Unknown43
Graft loss37 (20.6%)24 (34.3%)0.023
Patient status Alive:Dead143:3747:230.041
Graft survival, yr0.024
195.00%94.30%
387.20%81.40%
583.80%72.30%
Patient survival, yr0.045
195.60%95.70%
387.20%82.90%
583.80%73.80%
Disease-free survival, yr0.006
191.10%84.30%
385.00%75.70%
582.20%70.00%
Postoperative early complication by Clavien grading0.798
No9038
I3517
II164
IIIA196
IIIB113
IVA62
IVB00
V30
Figure 4
Figure 4 Overall survival of patients with preoperative alpha-fetoprotein ≤ 105 ng/mL and > 105 ng/mL. AFP: Alpha-fetoprotein. bP < 0.01.
Figure 5
Figure 5 Disease-free survival of patients with preoperative alpha-fetoprotein ≤ 105 ng/mL and > 105 ng/mL. AFP: Alpha-fetoprotein. bP < 0.01.
DISCUSSION

AFP has been used as a tumor marker for HCC. Its elevation depends on pathological characteristics, including tumor size and degree of differentiation of tumor cells. It is a well-established surrogate of tumor biology, as it correlates with histological grading and vascular invasion[12,13,22,23]. The presence of microvascular invasion and poor differentiation of tumor cells are associated with recurrence in patients within and beyond various transplant criteria[24-29]. However, most of the time, preoperative histological results are not available, and therefore prediction of disease recurrence and overall survival cannot be made preoperatively.

AFP is an oncogene protein produced by HCC. Currently, an AFP level of > 400 ng/mL together with a liver mass with characteristic features is a diagnostic feature of HCC. Serum AFP is a well-established prognostic marker of increased tumor virulence in HCC[12,13,30-35]. It has also been shown to be associated with increased risk of waitlist dropout[13,36,37] and post-LT recurrence[12,30-35,38-40]. AFP level has been integrated into a number of transplant criteria, including the Hangzhou criteria[41], the extended Toronto criteria[42], the “total tumor volume”[43], and the Kyoto criteria[44].

Regarding LT for HCC, disease recurrence is a major concern. Extrahepatic metastasis is a clear contraindication to LT as it represents systemic disease, which cannot be cured by LT. The presence of macrovascular invasion has been shown to be an independent risk factor for recurrence and associated with worsened survival[25,45], and therefore is considered a contraindication to LT in the Milan, UCSF and “up-to-seven” criteria[1,5,14]. Poor tumor biology (poor cellular differentiation and presence of microvascular invasion) is associated with an increased risk of tumor recurrence. However, most of the time, the tumor biology cannot be known before operation. Liver biopsy of the target lesion can be an important tool for identifying tumor differentiation and microvascular invasion. It has been proposed that liver biopsy should be included into the Toronto criteria for any number and any size of HCC lesion[42]. While AFP is known to be a well-established surrogate of tumor biology for its correlation with histological grading and vascular invasion[12,13,22,23], it has been used for risk stratification, with elevation of AFP associated with a higher incidence of disease recurrence. Unfortunately, there is no exact cut-off value as an absolute value for contraindication to LT.

AFP is associated with vascular invasion and intrahepatic metastasis is not expressed in well-differentiated HCC[46]. AFP is considered an independent prognostic factor which correlates with histological differentiation[12,47]. This was also reflected in our patients. In Group A, the median time to operation was longer and the outcome was better, suggesting that this group had more favorable tumor biology. Microvascular invasion has been proven to be a strong predictor of outcome (liver resection or LT) for HCC patients[48-51]. Unfortunately, tumor differentiation and microvascular status require histological proof, and most of the time they can only be known after operation. Our patients were divided into 3 main groups, with normal, high and very high preoperative AFP levels. Results showed that higher AFP level was associated with tumor recurrence and correlated with tumor differentiation and microvascular invasion. It was apparent that when the AFP value was lower, the chance of poor differentiation and vascular permeation was also lower. However, this could have been affected by the fact that Group C had more patients beyond the Milan criteria. ROC analysis was performed to assess the ability of preoperative AFP in predicting HCC recurrence after LT. According to C-index analysis based on ROC, the optimal cut-off value was set at 54 ng/mL. However, on further discriminative analysis, 105 ng/mL was set as the cut-off value and it demonstrated significant survival difference despite same amounts of patients who were within the Milan and UCSF criteria. This suggested the importance of using AFP as one of the preoperative surrogate markers to evaluate LT candidates, as it represents additional information on identifying high-risk patients preoperatively so as to predict the risk of recurrence and to let patients have realistic anticipation regarding their long-term outcomes.

HCC patients within the Milan criteria consistently have a 10%-15% risk of disease recurrence after LT[1]. However, strictly following the rules would turn down a substantial number of patients who could benefit from LT and get a cure, even with reasonable disease-free and overall survival. Therefore, allowance is given mainly based on tumor number and size[5,35,52]. It is hoped that further developed selection tools with expanded criteria can identify patients with low risk of recurrence. Applying AFP cut-off could have resulted in the exclusion of certain patients who could have reasonable survival but were at higher risk of tumor recurrence as compared with patients with a lower AFP level. However, using AFP alone to predict the subsequent disease course would result in bias in selection of patients for LT, since patients with the same AFP level can have similar disease-free survival as well as overall survival. Therefore, in listing patients for LT, other factors should also be considered, such as established criteria and tumor status assessed by PET. Fluorine-18-fluorodeoxyglucose PET is able to pick up poorly differentiated HCC[53], while 11C-choline PET has strong avidity for HCC, particularly well differentiated and moderately differentiated tumors[54-56]. Both ways provide preoperative information without the need for biopsy of the liver tumor. For patients who have poor liver function that precludes liver resection, ablative therapy and transarterial chemoembolization, palliative treatment would be the only option if they are denied LT. For these patients, LT should not be ruled out if a relatively inferior survival outcome is acceptable to them. Since LDLT is almost exclusively performed among family members, oftentimes they would accept a relatively inferior survival outcome of the recipient, given a reasonable donor operative outcome.

The findings of this study may not be universally applicable, as AFP 105 ng/mL is a value calculated from our cohort of 250 patients. Moreover, this is a retrospective cohort study with inevitable selection bias. Furthermore, the different levels of AFP (normal, high and very high) were arbitrarily defined. Before adopting AFP as a decision-making tool based on current selection criteria, we have to balance the risk of disease recurrence (hence overall survival) and the patients’ expectation. Still, it is hoped that this study can shed some light on the importance of adding AFP to the armamentarium of assessment tools for LT listing.

HCC patients with a high preoperative AFP level had inferior survival after LT. AFP level of 54 ng/mL was associated with disease recurrence, and AFP level of 105 ng/mL was found to be the cut-off value for overall survival difference.

ARTICLE HIGHLIGHTS
Research background

Liver transplantation is the best treatment option for hepatocellular carcinoma. However, only patients’ tumor criteria should fit the current adopted selection criteria. Most of the criteria are morphological descriptions, including size and number, with the recently added alpha-fetoprotein in some of the updated criteria.

Research motivation

We hoped to identify the cutoff value of alpha-fetoprotein in predicting disease recurrence and overall survival. Apart from using size and number as the selection criteria for liver transplantation, the additional use of alpha-fetoprotein might be able to give practical prediction of disease recurrence.

Research objectives

The objective of this study is to investigate the impact of alpha-fetoprotein on the long-term recurrence rate and overall survival of recipients of liver transplantation for hepatocellular carcinoma.

Research methods

Data of adult patients who received liver transplantation for hepatocellular carcinoma at our hospital from January 2000 to December 2013 were reviewed. Data of included patients were analyzed. We defined the different levels of alpha-fetoprotein as normal (< 10 ng/mL), high (≥ 10 to < 400 ng/mL) and very high (≥ 400 ng/mL). The patients were divided into these 3 groups accordingly. Group comparison was then made.

Research results

Alpha-fetoprotein level was normal in 83 patients, high in 131 patients, and very high in 36 patients. The commonest etiology was hepatitis-B-related cirrhosis. The Model for End-stage Liver Disease scores in these groups were similar (median, 13 vs 13 vs 12; P = 0.745). Patients with normal alpha-fetoprotein level had longer time to operation (median, 94 vs 31 vs 35 d; P = 0.001). The groups were similar in hospital mortality (P = 0.626) and postoperative complication (P = 0.702). Pathology of explants showed that the 3 groups had similar numbers of tumor nodules, but patients with very high alpha-fetoprotein level had bigger tumors (P = 0.003). This group also had a bigger proportion of patients who were beyond Milan criteria (P = 0.010). Poor differentiation and vascular permeation were commoner in this group (P = 0.017 and P = 0.003 respectively). It also had poorer 5-year overall survival (P = 0.029) and disease-free survival (P = 0.007). Receiver operating characteristic area under the curve for alpha-fetoprotein in predicting tumor recurrence was 0.685. The selected cut-off value was 54 ng/mL (C-index 0.685; 95%CI: 0.592-0.779; sensitivity 0.595; specificity 0.687). On discriminative analysis, alpha-fetoprotein value of 105 ng/mL was shown to affect the overall survival of the patients.

Research conclusions

This study showed that patients with high preoperative alpha-fetoprotein levels had poorer post-transplant survival. An alpha-fetoprotein level of 54 ng/mL was associated with disease recurrence, and 105 ng/mL was found to be the cutoff value for overall survival difference. These findings would be useful when considering liver transplantation for patients with a high alpha-fetoprotein level. Currently, there is no definite cutoff value of alpha-fetoprotein for ideal oncological outcomes in hepatocellular carcinoma. With the above alpha-fetoprotein values, superior long-term disease-free and overall survival will be achievable if liver transplantation is offered to patients with a lower preoperative alpha-fetoprotein level. The additional use of alpha-fetoprotein will allow better prediction of the long-term survival outcome, and hence affect the future practice in selection of patients for liver transplantation.

Research perspectives

Selection of patients for liver transplantation should not be based on morphological criteria alone. Other biomarkers such as alpha-fetoprotein should be added to the criteria currently used.

Footnotes

Manuscript source: Invited manuscript

Specialty type: Gastroenterology and hepatology

Country of origin: China

Peer-review report classification

Grade A (Excellent): 0

Grade B (Very good): 0

Grade C (Good): C, C

Grade D (Fair): 0

Grade E (Poor): 0

P- Reviewer: Marino IRR, Qin JM S- Editor: Cui LJ L- Editor: A E- Editor: Li RF

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