Simplified three-dimensional culture system for long-term expansion of embryonic stem cells
Christina McKee, Mick Perez-Cruet, Ferman Chavez, G Rasul Chaudhry, OU-WB Institute for Stem Cell and Regenerative Medicine, Oakland University, Rochester, MI 48309, United States
Christina McKee, G Rasul Chaudhry, Department of Biological Sciences, Oakland University, Rochester, MI 48309, United States
Mick Perez-Cruet, Beaumont Health System, Royal Oak, MI 48073, United States
Ferman Chavez, Department of Chemistry, Oakland University, Rochester, MI 48309, United States
Author contributions: McKee C preformed the majority of experiments, and helped write the manuscript; Perez-Cruet M and Chavez F provided resources; Chavez F synthesized scaffold material; Perez-Cruet M and Chavez F were involved in the editing of the manuscript; Chaudhry GR designed and guided the study, and helped write the manuscript.
Supported by Oakland University and Oakland University-William Beaumont Institute for Stem Cell and Regenerative Medicine (OU-WB ISCRM).
Institutional review board statement: This research is conducted with cell lines purchased from ATCC and has been approved by the Oakland University Institutional Biosafety Committee (IBC protocol number: 1814).
Institutional animal care and use committee: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of Oakland University (IACUC protocol number: 14033).
Conflict-of-interest statement: None.
Data sharing statement: None.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. G Rasul Chaudhry, Department of Biological Sciences, Oakland University, 2200 North Squirrel Road, Rochester, MI 48309, United States. email@example.com
Telephone: +1-248-3703350 Fax: +1-248-3703586
Received: February 21, 2015
Peer-review started: February 22, 2015
First decision: April 27, 2015
Revised: May 27, 2015
Accepted: June 18, 2015
Article in press: June 19, 2015
Published online: August 26, 2015