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©2013 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Stem Cells. Oct 26, 2013; 5(4): 217-228
Published online Oct 26, 2013. doi: 10.4252/wjsc.v5.i4.217
Published online Oct 26, 2013. doi: 10.4252/wjsc.v5.i4.217
Insulin producing cells established using non-integrated lentiviral vector harboring PDX1 gene
Zahra Niki Boroujeni, Ahmad Aleyasin, Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran 14155-6343, Iran
Author contributions: Boroujeni ZN performed all of the experiments; Aleyasin A was the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis, designed and supervised the study, financed the study and provided the laboratory vital reagents and analytical tools, and was also involved in editing the manuscript.
Supported by National Institute of Genetic Engineering and Biotechnology, Ministry of Science Research and Technology, Tehran, Iran
Correspondence to: Dr. Ahmad Aleyasin, DVM, MSc, PhD, Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran 14155-6343, Iran. sogand@nigeb.ac.ir
Telephone: +98-21-44580383 Fax: +98-21-44580395
Received: May 25, 2013
Revised: August 27, 2013
Accepted: September 18, 2013
Published online: October 26, 2013
Revised: August 27, 2013
Accepted: September 18, 2013
Published online: October 26, 2013
Core Tip
Core tip: Common treatments for diabetes mellitus are based on insulin injections and pancreas transplantation, limited by hypoglycemia, shortage of donors, immunosuppression and organ rejection. Cell therapy using human adipose tissue derived stem cells (hADSCs) offers a novel strategy for diabetes treatment without tumor formation and ethical concerns. Different viral vectors have been used for pancreatic differentiation. However, integration of provirus into host chromatin has induced insertional mutagenesis and malignancy. This is the first study to investigate the application of non-integrated Lentiviral vectors harboring PDX1 for differentiation of hADSCs into insulin producing cells and its usage in treatment of diabetic rats.