Insulin producing cells established using non-integrated lentiviral vector harboring PDX1 gene

Abstract

AIM: To investigate reprogramming of human adipose tissue derived stem cells into insulin producing cells using non-integrated lentivirus harboring PDX1 gene.

METHODS: In this study, human adipose tissue derived stem cells (hADSCs) were obtained from abdominal adipose tissues by liposuction, selected by plastic adhesion, and characterized by flow cytometric analysis. Human ADSCs were differentiated into adipocytes and osteocytes using differentiating medium to confirm their multipotency. Non-integrated lentiviruses harboring PDX1 (Non-integrated LV-PDX1) were constructed using specific plasmids (pLV-HELP, pMD2G, LV-105-PDX1-1). Then, hADSCs were transduced with non-integrated LV-PDX1. After transduction, ADSCs^PDX1+ were cultured in high glucose DMEM medium supplement by B27, nicotinamide and βFGF for 21 d. Expressions of PDX1 and insulin were detected at protein level by immunofluorescence analysis. Expressions of PDX1, neurogenin3 (Ngn3), glucagon, glucose transporter2 (Glut2) and somatostatin as specific marker genes were investigated at mRNA level by quantitative RT-PCR. Insulin secretion of hADSCs^PDX1+ in the high-glucose medium was detected by electrochemiluminescence test. Human ADSCs^PDX1+ were implanted into hyperglycemic rats.

RESULTS: Human ADSCs exhibited their fibroblast-like morphology and made colonies after 7-10 d of culture. Determination of hADSCs identified by FACS analysis showed that hADSCs were positive for mesenchymal cell markers and negative for hematopoietic cell markers that guaranteed the lack of hematopoietic contamination. In vitro differentiation of hADSCs into osteocytes and adipocytes were detected by Alizarin red and Oil red O staining and confirmed their multilineage differentiation ability. Transduced hADSCs^+PDX1 became round and clusters in the differentiation medium. The appropriate expression of PDX1 and insulin proteins was confirmed using immunocytochemistry analysis. Significant expressions of PDX1, Ngn3, glucagon, Glut2 and somatostatin were detected by quantitative RT-PCR. hADSCs^PDX1+ revealed the glucose sensing ability by expressing Glut2 when they were cultured in the medium containing high glucose concentration. The insulin secretion of hADSCs^PDX1+ in the high glucose medium was 2.32 μU/mL. hADSCs^PDX1+ implantation into hyperglycemic rats cured it two days after injection by reducing blood glucose levels from 485 mg/dL to the normal level.

CONCLUSION: Human ADSCs can differentiate into IPCs by non-integrated LV-PDX1 transduction and have the potential to be used as a resource in type 1 diabetes cell therapy.

Keywords: Diabetes mellitus, Human adipose tissue derived stem cells, Non-integrated lentiviruses, PDX1, Insulin producing cells

Core tip: Common treatments for diabetes mellitus are based on insulin injections and pancreas transplantation, limited by hypoglycemia, shortage of donors, immunosuppression and organ rejection. Cell therapy using human adipose tissue derived stem cells (hADSCs) offers a novel strategy for diabetes treatment without tumor formation and ethical concerns. Different viral vectors have been used for pancreatic differentiation. However, integration of provirus into host chromatin has induced insertional mutagenesis and malignancy. This is the first study to investigate the application of non-integrated Lentiviral vectors harboring PDX1 for differentiation of hADSCs into insulin producing cells and its usage in treatment of diabetic rats.