Basic Study
Copyright ©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. May 26, 2025; 17(5): 104116
Published online May 26, 2025. doi: 10.4252/wjsc.v17.i5.104116
Optimal good manufacturing practice-compliant production of dental follicle stem cell sheet and its application in Sprague-Dawley rat periodontitis
Jia-Lu Yu, Chao Yang, Li Liu, An Lin, Shu-Juan Guo, Wei-Dong Tian
Jia-Lu Yu, An Lin, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, Sichuan Province, China
Chao Yang, Department of Product Development, Chengdu Shiliankangjian Biotechnology Co., Ltd, Chengdu 610041, Sichuan Province, China
Li Liu, Engineering Research Center of Oral Translational Medicine, National Clinical Research Center for Oral Diseases, Departments of 5 Periodontics and 6 Oral and Maxillofacial Surgery, Sichuan University, Chengdu 610041, Sichuan Province, China
Shu-Juan Guo, Department of Periodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
Wei-Dong Tian, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Engineering Research Center of Oral Translational Medicine, National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
Co-corresponding authors: Shu-Juan Guo and Wei-Dong Tian.
Author contributions: Yu JL, Yang C, Guo SJ, and Tian WD contributed to the conceptualization and methodology; Yu JL, Liu L, and Lin A were involved in the investigation and formal analysis; Yu JL wrote original draft; Yang C and Guo SJ contributed to the revision of this manuscript; Guo SJ and Tian WD provided daily supervision, project administration, and funding acquisition, they contributed equally to this manuscript as co-corresponding authors.
Supported by National Key Research and Development Program of China, No. 2021YFA1100600 and No. 2022YFA1104400.
Institutional review board statement: The study was reviewed and approved by the Ethics Committee of the West China School of Stomatology, Sichuan University, (Approval No. WCHSIRB-D-2024-237).
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the West China School of Stomatology, Sichuan University, IACUC protocol number: Protocol No. WCHSIRB-D-2024-368.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: The data supporting the findings of this study are available from the corresponding author upon reasonable request.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Wei-Dong Tian, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Engineering Research Center of Oral Translational Medicine, National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, No. 14, Section 3, Renmin South Road, Wuhou District, Chengdu 610041, Sichuan Province, China. drtwd@sina.com
Received: December 11, 2024
Revised: February 27, 2025
Accepted: April 18, 2025
Published online: May 26, 2025
Processing time: 167 Days and 5.8 Hours
Core Tip

Core Tip: The existing culture method for dental follicle stem cell (DFSC) sheets is crude and not uniform. For clinical application, it is necessary to produce good manufacturing practice compliant DFSC sheets and determine a uniform culture method that can maintain the best performance of DFSC sheets and consume the least. In this study, the culture conditions of the good manufacturing practice-compliant DFSC sheet were optimized, focusing on four key factors: Cell passage, cell concentration, L-ascorbic acid content, and culture duration. Compared with the DFSC suspension, the cell sheet demonstrated superior capacities. These findings offer valuable insights and guidance for the clinical application of cell sheets.