Ooi YY, Rahmat Z, Jose S, Ramasamy R, Vidyadaran S. Immunophenotype and differentiation capacity of bone marrow-derived mesenchymal stem cells from CBA/Ca, ICR and Balb/c mice. World J Stem Cells 2013; 5(1): 34-42 [PMID: 23362438 DOI: 10.4252/wjsc.v5.i1.34]
Corresponding Author of This Article
Dr. Sharmili Vidyadaran, PhD, Immunology Laboratory, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia. sharmili@medic.upm.edu.my
Article-Type of This Article
Brief Article
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Yin Yin Ooi, Zul’atfi Rahmat, Shinsmon Jose, Rajesh Ramasamy, Sharmili Vidyadaran, Immunology Laboratory, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia
Yin Yin Ooi, Department of Anatomy, Yong Loo Lin School of Medicine, NUS, MD10, 4 Medical Drive, Singapore 117597, Singapore
Author contributions: Ooi YY performed the research and analysed the data; Rahmat Z assisted with the differentiation assays; Jose S assisted with the immunophenotyping; Ramasamy R provided technical advice on culture and characterisation of the mesenchymal stem cells; Vidyadaran S conceived the study, designed the experiments and wrote the manuscript.
Supported by The Research University Grant Scheme UPM, 04-02-10-0924RU; and Exploratory Research Grant Scheme, Ministry of Higher Education, ERGS/1/2012/5527106
Correspondence to: Dr. Sharmili Vidyadaran, PhD, Immunology Laboratory, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia. sharmili@medic.upm.edu.my
Telephone: +60-3-89472376
Received: February 8, 2012 Revised: November 14, 2012 Accepted: December 20, 2012 Published online: January 26, 2013
Abstract
AIM: To assess the capacity to isolate and expand mesenchymal stem cells (MSC) from bone marrow of CBA/Ca, ICR and Balb/c mice.
METHODS: Bone marrow of tibia and femur were flushed, cultured and maintained in supplemented Dulbecco’s modified Eagle’s medium. MSC immunophenotype of cultures were tracked along increasing passages for positivity to CD106, Sca-1 and CD44 and negativity to CD45, CD11b and MHC class II. Differentiation capacity of MSC towards osteogenic and adipogenic lineages were also assessed.
RESULTS: MSC were successfully cultured from bone marrow of all 3 strains, albeit differences in the temporal expression of certain surface antigens. Their differentiation into osteocytes and adipocytes were also observed. MSC from all 3 mouse strains demonstrated a shift from a haematopoietic phenotype (CD106-CD45+CD11b+Sca-1low) to typical MSC phenotype (CD106+CD45-CD11b-Sca-1high) with increasing passages.
CONCLUSION: Information garnered assists us in the decision of selecting a mouse strain to generate MSC from for downstream experimentation.
