Basic Study
Copyright ©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. May 26, 2025; 17(5): 105394
Published online May 26, 2025. doi: 10.4252/wjsc.v17.i5.105394
Cell-free extracts from human fat tissue attenuate ischemic injury in cardiomyocytes in a murine model
Tian-Yun Yang, Yi Sun, Wen-Jie Zhang, Chang-Qian Wang, Jin Zhou
Tian-Yun Yang, Yi Sun, Chang-Qian Wang, Jin Zhou, Department of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
Wen-Jie Zhang, Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
Co-first authors: Tian-Yun Yang and Yi Sun.
Co-corresponding authors: Chang-Qian Wang and Jin Zhou.
Author contributions: Wang CQ and Zhou J were responsible for study conceptualization and design, and they made the same contribution as co-corresponding authors; Yang TY and Sun Y developed the methodology and wrote the paper; Zhang WJ provided fat extract; Yang TY and Zhang WJ made the same contribution as co-first authors of this manuscript. All authors read and approved the final paper.
Supported by National Natural Science Foundation of China, No. 82200270; and the Vascular Disease Discipline Cluster, No. SKQJS202402.
Institutional review board statement: The study was approved by the Ethics Committee of Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China, approval No. [2018]37.
Institutional animal care and use committee statement: The research was performed in alignment with the guidelines set forth in the “Guide for the Care and Use of Laboratory Animals” as published by the United States National Institutes of Health (NIH publication No. 85-23, revised 2011) and received approval from the Animal Experiment Ethics Committee at Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine (Approval No. SH9H-2021-A839-SB).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: All data are available with the corresponding author upon reasonable request.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Chang-Qian Wang, Professor, Department of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, No. 280 Mohe Road, Baoshan District, Shanghai 200011, China. wangcqdr1218@163.com
Received: January 21, 2025
Revised: February 27, 2025
Accepted: April 15, 2025
Published online: May 26, 2025
Processing time: 125 Days and 22.6 Hours
Abstract
BACKGROUND

Ischemic heart disease ranks among the foremost contributors to mortality worldwide. Myocardial infarction injury poses a prevalent challenge in current therapies. Studies have shown that mesenchymal stem cell transplantation increases cytokine release, reduces myocardial cell necrosis, and improves left ventricular function; thus, it can be used to understand protective mechanisms. Fat extract (FE) derived from mesenchymal stem cell therapy contains high levels of paracrine factors.

AIM

To study the effects of FE on myocardial injury and its mechanism of action.

METHODS

A mouse model of myocardial infarction and a hypoxic model of neonatal rat cardiomyocytes (CMs) were established to evaluate the effects of FE.

RESULTS

FE exhibited an inhibitory effect on CM apoptosis and improved left ventricular function. This protective effect of FE on CMs was mediated, in part, by the activation of the phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin signaling pathway.

CONCLUSION

Our findings showed that FE could be a new treatment to protect CMs in ischemic heart disease.

Keywords: Ischemic heart disease; Myocardial infarction; Ischemia injury; Fat extract; Phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin signaling pathway

Core Tip: Fat extract derived from mesenchymal stem cell therapy demonstrates protective effects on cardiomyocytes under ischemic conditions by inhibiting cardiomyocyte apoptosis and improving left ventricular function. This protection is mediated through the activation of the phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin signaling pathway, suggesting fat extract as a potential new treatment for ischemic heart disease.