Inhibitory effects of c9,t11-conjugated linoleic acid on invasion of human gastric carcinoma cell line SGC-7901

Table 1 Primer sequence and size of expected PCR products

Primer	Sequence	Lengh (bp)
β-actin sense	5’-AAGGATTCCTATGTGGGC-3’	532
antisense	5’-CATCTCTTGCTCGAAGTC-3’
TIMP-1 sense	5’-CTGTTGGCTGTGAGGAATGCACAG-3’	106
antisense	5’-TTCAGAGCCTTGGAGGAGCTGGTC-3’
TIMP-2 sense	5’-AGACGTAGTGATCGGGCCA-3’	490
antisense	5’-GTACCACGCGCAAGAACCT-3’

Table 2 Effect of c9,t11-CLA on invasive ability of SGC-7901 cells

Groups	Invasive cell number (-x±s)	Inhibitory frequency (%)
200 μmol/L	23.2 ± 2.9a	53.7
100 μmol/L	29.6 ± 3.3a	40.9
50 μmol/L	35.4 ± 2.8a	29.3
25 μmol/L	44.9 ± 3.1	10.4
Negative control	50.1 ± 4.6	0

^aP < 0.01, compared with the negative control group.

Table 3 Effect of c9,t11-CLA on migration ability of SGC-7901 cells

Groups	Cell number (-x±s)	Inhibitory frequency (%)
200 μmol/L	50.8 ± 3.3a	16.0
100 μmol/L	52.5 ± 3.1	13.2
50 μmol/L	54.4 ± 3.9	10.1
25 μmol/L	55.0 ± 4.4	10.1
Negative control	60.5 ± 4.2	0

^aP < 0.05, compared with the negative control group.