Expression of ECRG4, a novel esophageal cancer-related gene, downregulated by CpG island hypermethylation in human esophageal squamous cell carcinoma

Table 1 The expression and methylation of ECRG 4 in ESCC

Cases	Gender	Pathological stage	Expression		Methylation
			Normal	Cancer	Normal	Cancer
N1	Fa		++c		-
N2	F		++		-
1	Mb	Moderate	++	+d	-f	+g
2	F	Moderate	+	-	-	+
3	M	Moderate	++	++	-	-
4	M	Moderate	++	++	-	-
5	F	Moderate	++	-e	-	+
6	F	Moderate	+	+	+	+
7	M	Moderate	++	++	-	-
8	F	Poor	++	-	-	+
9	M	Moderate	++	++	-	-
10	M	Poor	++	-	-	+
11	F	Moderate	+	+	+	+
12	F	Moderate	++	-	-	+
13	F	Moderate	++	++	-	-
14	M	Moderate	++	+	-	+
15	M	Moderate	++	-	-	+
16	F	Moderate	++	-	-	+
17	M	Moderate	-	-	+	-
18	F	Moderate	++	+	-	-
19	M	Moderate	++	+	-	-
20	M	Moderate	++	-	-	+

^a, Female;

^b, Male;

^c++, high expression;

^d+, low expression;

^e-, unexpression;

^f-, unmethylation;

^g+, methylation.

Table 2 The expression and methylation of ECRG 4 in cell lines

Cell lines	Expression	Methylation
NEC	-a	-c
EC109	-	+d
EC9706	-	+
GLC	-	+
Hek293	+b	-

^a-, unexpression;

^b+, expression;

^c-, unmethylation;

^d+, methylation.