Deletion of Helicobacter pylori vacuolating cytotoxin gene by introduction of directed mutagenesis

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Oct 15, 2003 (publication date) through Apr 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 15, 2003; 9(10): 2251-2257
Published online Oct 15, 2003. doi: 10.3748/wjg.v9.i10.2251

Table 1 Primers for amplifying km^r, LA and RA

Primer	Sequence (5’ to 3’)	Site	Coordinates
la1	GGGGTACCCTTTTGAGCCTTTAGTT	Kpn I	cysS bp 36-54
la2	CGGAATTCTCCTTTCTTTTTGTAAAAC	EcoR I	HPU07145 bp 382-400
km^r1	CGGAATTCATGATTGAACAAGATGGATTG	EcoR I	pEGFP-N1 bp 2629-2649
km^r2	CGGGATCCTCAGAAGAACTCGTCAAG	BamH I	pEGFP-N1 bp 3406-3423
ra1	CGGGATCCATCGCCCTCTGGTTTCTC	BamH I	HPU07145 bp 437-454
ra2	GCTCTAGACACCCACTTGATTATTCACTCT	Xba I	HPU07145 bp 1786-1807

Citation: Yuan JP, Li T, Shi XD, Hu BY, Yang GZ, Tong SQ, Guo XK. Deletion of Helicobacter pylori vacuolating cytotoxin gene by introduction of directed mutagenesis. World J Gastroenterol 2003; 9(10): 2251-2257
URL: https://www.wjgnet.com/1007-9327/full/v9/i10/2251.htm
DOI: https://dx.doi.org/10.3748/wjg.v9.i10.2251