PELA microspheres loaded H. pylori lysates and their mucosal immune response

Table 1 The properties of the PELA-Hp microspheres

Lot	PELA[η] (dL/g)*	Mean dimeter(μm)	Protein loading (%,w/w)	Encapsulation efficiency (%,w/w)
MS-2	0.2083	3.20	5.53	82.2
MS-3	0.3054	3.53	5.40	74.9
MS-4	0.3329	4.05	5.27	79.5

*The inherent viscosity of PELA is measured in tetrahydrofu-ran at 25 °C.

Table 2 Comparison of sIgA titres in saliva at different stages after oral immunization (^-x ± s)

Groups	Week 3	Week 5	Week 8
PBS	0.081 ± 0.026	0.074 ± 0.023	0.080 ± 0.011
Ag	0.197 ± 0.177	0.199 ± 0.093	0.225 ± 0.029
MS-0	0.090 ± 0.041	0.071 ± 0.013	0.110 ± 0.046
MS-1	0.416 ± 0.183	0.567 ± 0.136	0.891 ± 0.215
MS-2	0.399 ± 0.179	0.608 ± 0.209	0.882 ± 0.264
MS-3	0.346 ± 0.126	0.674 ± 0.128	0.957 ± 0.238
MS-4	0.304 ± 0.105	0.523 ± 0.211	0.944 ± 0.143

Table 3 Immune response induced with PELA-Hp microspheres at week 8 (n = 20, ^-x ± s)

Groups	PP sIgA-ASCs	(1 × 104) IgG-ASCs	Stomach sIgA-ASCs	(1 × 103) IgG-ASCs	Gut sIgA
PBS	0.13 ± 0.04	0.16 ± 0.07	0.19 ± 0.08	0.23 ± 0.06	0.234 ± 0.08
Ag	0.27 ± 0.11	0.29 ± 0.09	0.35 ± 0.11	0.36 ± 0.15	0.34 ± 0.17
MS-0	0.16 ± 0.07	0.17 ± 0.05	0.15 ± 0.06	0.21 ± 0.07	0.25 ± 0.08
MS-1	6.58 ± 1.38	3.78 ± 0.82	4.17 ± 1.09	0.74 ± 0.29	1.75 ± 0.46
MS-2	6.92 ± 1.77	4.02 ± 0.93	3.80 ± 1.34	1.02 ± 0.44	1.85 ± 0.38
MS-3	6.77 ± 1.46	3.56 ± 1.10	4.12 ± 0.83	1.81 ± 0.73	1.62 ± 0.81
MS-4	5.92 ± 1.92	3.47 ± 0.82	3.65 ± 1.04	1.88 ± 0.23	1.71 ± 0.70