Viral Hepatitis
Copyright ©The Author(s) 2002.
World J Gastroenterol. Aug 15, 2002; 8(4): 699-702
Published online Aug 15, 2002. doi: 10.3748/wjg.v8.i4.699
Table 1 Nucleotide sequences and positions of primers
PrimersSequence (5’-3’)Positionband sizes (bp)
CE (+)ATTGGATTGGCCATCCGGTG620-639
P (-)1TCCACCACCACCCTCTCGACTC1169-1190571
P (-)2GCATTACACTGTACGTGCAC1271-1290671
C (-)1CACCAACACCCATACCTGCA1470-1489870
C (-)2GCGCACATTGGCGCAATTGTG1683-17031084
E (-)1CGCACCCCATCTCAGCTTCA1330-1349730
E (-)2TTCATTGTCGGCAAACCTTGA1727-17471128
H (-)CCAATCTTCCTGATCCAAAGC1557-1577
H (+)1AACCCTACACCTTTCCAACA1146-1165432
H (+)2GATTCATTCTGCAGATTGGC989-1008589
Table 2 Sensitivity of semi-nested-PCR for different viruses
VirusesVirus concentration (PFU)
10310210110010-1
Poliovirus++++ (2.4)-
Coxsackie virus B3++++ (2.1)-
Echovirus 9++++ (6.0)-
Three viruses+++ (10.5)--