Original Articles
Copyright ©The Author(s) 2001.
World J Gastroenterol. Jun 15, 2001; 7(3): 345-351
Published online Jun 15, 2001. doi: 10.3748/wjg.v7.i3.345
Table 1 Specific inhibition of SMMC-7721 human hepatoma cell growth by alpha-fetoprotein (AFP) antisense phosphorothioate oligodeoxyribonucleotides (S-ODNs) in vitro
Concentration (μmol/L)Hepatoma cell growth (absorbance at 570 nm)
SenseAntisenseOligo dT15
00.83 ± 0.100.83 ± 0.100.83 ± 0.10
6.30.84 ± 0.080.50 ± 0.08 (39.8)a0.82 ± 0.11
12.50.76 ± 0.060.44 ± 0.04 (46.9)a0.87 ± 0.07
25.00.72 ± 0.130.42 ± 0.03 (49.4)a0.88 ± 0.18
Table 2 Inhibition of AFP protein expression and apoptosis induction in SMMC-7721 human hepatoma cells by AFP antisense S-ODNs in vitro
Concentration (μmol/L)AFP protein expression (absorbance at 460 nm)
Apoptosis (%)
AntisenseSenseOligo-dT15
00.32 ± 0.040.32 ± 0.040.32 ± 0.047.3 ± 5.2
6.30.22 ± 0.03 (31.5)aNDNDND
12.50.19 ± 0.05 (40.6)aNDND26.7 ± 7.8a
25.00.15 ± 0.02 (53.1)a0.31 ± 0.060.31 ± 0.0247.9 ± 7.5a
Table 3 Therapeutic efficacy of AFP S-ODNs in SMMC-7721 hepatoma bearing nude mice
Group (mg/day × day)Mean tumor weight (mg)
Toxic deathsAFP content (% inhibition)
0*2468101214
Control (0)96.7 ± 51.1191.4 ± 48.3336.9 ± 67.8504.8 ± 80.2636.3 ± 68.8823.9 ± 187.61056.7 ± 182.01201.6 ± 208.00/6-
Antisense (0.25 ± 8)83.1 ± 33.7145.2 ± 43.8 (24.2)215.8 ± 49.6 (35.9)a286.5 ± 49.2 (43.2)a356.7 ± 52.5 (43.9)a520.0 ± 56.1 (36.9)a653.1 ± 128.8 (38.2)a776.6 ± 61.3 (35.4)a0/668.9a
Antisense (0.50 ± 8)84.8 ± 34.6130.3 ± 36.9 (31.9)a202.3 ± 47.9 (39.9)a254.2 ± 43.2 (49.6)a310.8 ± 56.9 (51.2)a449.9 ± 108.3 (45.4)a593.9 ± 72.3 (43.8)a737.3 ± 146.7 (38.6)a0/673.8a
Sense (0.25 ± 8)100.5 ± 33.5201.4 ± 44.2339.1 ± 71.1510.4 ± 114.1629.6 ± 109.4829.5 ± 164.51034.9 ± 198.11191.9 ± 181.30/63.4
Sense (0.50 ± 8)86.9 ± 25.6187.7 ± 42.3359.7 ± 59.6509.5 ± 111.8659.7 ± 101.7837.7 ± 147.31011.0 ± 142.91232.3 ± 149.00/60
Table 4 Reversal of SMMC-7721 human hepatoma cell growth inhibition of AFP antisense S-ODNs by hybridization competition in vitro
Antisense (μmol/L)Sense (μmol/L)Hepatoma cell growth (absorbance at 570 nm)Suppression (%)
001.00 ± 0.08
0100.95 ± 0.075
1000.46 ± 0.0254
10100.51 ± 0.0549
101000.95 ± 0.055