N-linked glycoproteomic profiling in esophageal squamous cell carcinoma

Table 1 Summary of high-mannose N-linked glycoproteins in esophageal squamous cell carcinoma by 2-dimensional gel electrophoresis

Spot No.	Accession	Protein identity (Theoretical MW/pI)	Ratio	P value	Scores	Unique Peptides	N-glycosylation sites
608	ENOA_HUMAN	Alpha-enolase (47.1/7.7)	2.2 (↑)	0.008	2050.2 (M:2050.2)	33	1 (102)
638	CATD_HUMAN	Cathepsin D (44.5/6.1)	2.6 (↑)	0.009	1351.8 (M:1351.8)	20	2 (134, 263)
645	G3P_HUMAN	Glyceraldehyde-3-phosphate dehydrogenase (36/9.3)	3.2 (↑)	0.009	319.9 (M:319.9)	4	0
646	G3P_HUMAN	Glyceraldehyde-3-phosphate dehydrogenase (36/9.3)	5.2 (↑)	0.002	611.5 (M:611.5)	10	0
648	G3P_HUMAN	Glyceraldehyde-3-phosphate dehydrogenase (36/9.3)	8.9 (↑)	0.009	730.1 (M:730.1)	14	0
660	CO1A2_HUMAN	Collagen alpha-2(I) chain (129.2/9.7)	1.7 (↑)	0.008	1207.8 (M:1207.8)	20	1 (207)
664	CATD_HUMAN	Cathepsin D (44.5/6.1)	12.5 (↑)	0.005	1337.1 (M:1337.1)	18	2 (134, 263)
668	ALDOA_HUMAN	Fructose-bisphosphate aldolase A (39.4/9.2)	1.6 (↑)	0.004	182.0 (M:182.0)	4	0
692	HSPB1_HUMAN	Heat shock protein beta-1 (22.8/6)	1.6 (↑)	0.009	1015.9 (M:1015.9)	15	0
694	TPIS_HUMAN	Triosephosphate isomerase (26.7/6.5)	1.8 (↑)	0.004	1277.6 (M:1277.6)	19	1 (233)
724	FABP5_HUMAN	Fatty acid-binding protein, epidermal (15.2/7.5)	2 (↑)	0.002	818.4 (M:818.4)	14	0
731	HPT_HUMAN	Haptoglobin (45.2/6.1)	2.1 (↑)	0.006	418.7 (M:418.7)	11	2 (207, 241)
734	HSPB1_HUMAN	Heat shock protein beta-1 (22.8/6)	1.5 (↑)	0.009	464.0 (M:464.0)	7	0
737	APOA1_HUMAN	Apolipoprotein A-I (30.8/5.5)	1.6 (↑)	0.002	200.4 (M:200.4)	4	0
738	COF1_HUMAN	Cofilin-1 (18.5/9.1)	1.5 (↑)	0.009	381.8 (M:381.8)	7	0
739	HBB_HUMAN	Hemoglobin subunit beta (16/6.9)	2.7 (↑)	0.008	720.5 (M:720.5)	10	0
753	CATD_HUMAN	Cathepsin D (44.5/6.1)	4.6 (↑)	0.009	702.0 (M:702.0)	13	2 (134, 263)
755	HBA_HUMAN	Hemoglobin subunit alpha (15.2/9.4)	3.9 (↑)	0.005	377.6 (M:377.6)	7	0
756	FIBB_HUMAN	Fibrinogen beta chain (55.9/9.3)	1.8 (↑)	0.009	3911.9 (M:3911.9)	55	0
791	FIBG_HUMAN	Fibrinogen gamma chain precursor (51.5/5.3)	1.5 (↑)	0.009	1356.7 (M:1356.7)	26	1 (78)
676	FIBB_HUMAN	Fibrinogen beta chain (55.9/9.3)	4.5 (↓)	0.006	1071.8 (M:1071.8)	18	0
678	CO3_HUMAN	Complement C3 (187/6)	6.2 (↓)	0.009	383.9 (M:383.9)	12	2 (85, 939)
688	HEMO_HUMAN	Hemopexin (51.6/6.6)	5.3 (↓)	0.002	698.2 (M:698.2)	13	2 (64, 240)
744	A1AT_HUMAN	Alpha-1-antitrypsin (46.7/5.3)	12.7 (↓)	0.009	784.8 (M:784.8)	14	3 (70, 107, 271)
748	HPT_HUMAN	Haptoglobin (45.2/6.1)	7.5 (↓)	0.008	1067.0 (M:1067.0)	19	2 (207, 241)
750	FIBB_HUMAN	Fibrinogen beta chain (55.9/9.3)	4.9 (↓)	0.009	3276.7 (M:3276.7)	48	0
772	A1AT_HUMAN	Alpha-1-antitrypsin (46.7/5.3)	1.8 (↓)	0.005	678.2 (M:678.2)	12	3 (70, 107, 271)
779	CLUS_HUMAN	Clusterin (52.5/5.9)	2.9 (↓)	0.009	619.3 (M:619.3)	10	3 (86, 103, 291)
801	FIBB_HUMAN	Fibrinogen beta chain (55.9/9.3)	2.1 (↓)	0.009	714.1 (M:714.1)	13	0
864	SODE_HUMAN	Extracellular superoxide dismutase [Cu-Zn] (25.8/6.2)	6.4 (↓)	0.004	798.6 (M:798.6)	12	1 (107)
902	APOA1_HUMAN	Apolipoprotein A-I (30.8/5.5)	4.3 (↓)	0.002	1206.2 (M:1206.2)	24	0
932	TRFE_HUMAN	Serotransferrin precursor (77/7)	3.8 (↓)	0.004	187.0 (M:187.0)	4	1 (630)
936	HPTR_HUMAN	Haptoglobin-related protein (39/6.7)	1.5 (↓)	0.002	226.4 (M:226.4)	4	1 (149)
1000	A1AT_HUMAN	Alpha-1-antitrypsin (46.7/5.3)	15.2 (↓)	0.006	1091.2 (M:1091.2)	20	3 (70, 107, 271)
1001	HPT_HUMAN	Haptoglobin (45.2/6.1)	9.8 (↓)	0.009	1054.6 (M:1054.6)	18	2 (207, 241)

Table 2 Summary of sialic N-linked glycoproteins identified in esophageal squamous cell carcinoma by 2-dimensional gel electrophoresis

Spot No.	Accession	Protein identity (Theoretical MW/pI)	Ratio	P value	Scores	Unique Peptides	N-glycosylation sites
3569	ENOA_HUMAN	Alpha-enolase (47.1/7.7)	2 (↑)	0.002	802.0 (M:802.0)	18	2 (17, 102)
3570	IGKC_HUMAN	Ig kappa chain C region (11.6/5.5)	2.4 (↑)	0.006	54.4 (M:54.4)	2	0
3612	TRFE_HUMAN	Serotransferrin (77/7)	1.5 (↑)	0.004	927.5 (M:927.5)	25	1 (630)
3687	KV305_HUMAN	Ig kappa chain V-III region WOL (11.7/9.5)	1.7 (↑)	0.009	125.4 (M:125.4)	2	0
3720	A1AT_HUMAN	Alpha-1-antitrypsin (46.7/5.3)	1.9 (↑)	0.002	49.3 (M:49.3)	2	3 (70, 107, 271)
4746	FIBB_HUMAN	Fibrinogen beta chain (55.9/9.3)	2.8 (↓)	0.004	817.3 (M:817.3)	17	0
4784	ACTB_HUMAN	Actin, cytoplasmic 1 (41.7/5.2)	3.4 (↓)	0.002	43.6 (M:43.6)	2	1 (12)
4948	HBB_HUMAN	Hemoglobin subunit beta (16/6.9)	1.8 (↓)	0.006	129.3 (M:129.3)	4	0
4967	ACTA_HUMAN	Actin, aortic smooth muscle (42/5.1)	1.7 (↓)	0.009	402.1 (M:402.1)	9	1 (14)
4985	FHR2_HUMAN	Complement factor H-related protein 2 (30.6/6)	2 (↓)	0.002	271.1 (M:271.1)	5	0
4998	1433Z_HUMAN	14-3-3 protein zeta/delta (27.7/4.6)	6.6 (↓)	0.009	62.1 (M:62.1)	2	2 (173, 224)
5010	FIBA_HUMAN	Fibrinogen alpha chain (94.9/5.6)	2.1 (↓)	0.002	83.3 (M:83.3)	3	3 (288, 419, 831)
5019	ACTH_HUMAN	Actin, gamma-enteric smooth muscle (41.8/5.2)	1.6 (↓)	0.009	92.7 (M:92.7)	3	1 (13)
5031	KV204_HUMAN	Ig kappa chain V-II region TEW (12.3/5.6)	1.5 (↓)	0.008	84.9 (M:84.9)	2	0
5057	CATB_HUMAN	Cathepsin B (37.8/5.9)	1.6 (↓)	0.009	84.8 (M:84.8)	2	2 (192, 289)
5069	FABP5_HUMAN	Fatty acid-binding protein, epidermal (15.2/7.5)	1.5 (↓)	0.005	88.7 (M:88.7)	2	0
5076	CRYAB_HUMAN	Alpha-crystallin B chain (20.1/6.9)	3.8 (↓)	0.009	240.1 (M:240.1)	6	0
5081	TAGL_HUMAN	Transgelin (22.6/9.4)	4.1 (↓)	0.009	134.7 (M:134.7)	3	0
5083	HSP71_HUMAN	Heat shock 70 kDa protein 1A/1B (70/5.4)	1.9 (↓)	0.004	105.8 (M:105.8)	3	1 (432)
5098	K1C17_HUMAN	Keratin, type I cytoskeletal 17 (48.1/4.8)	2.2 (↓)	0.002	198.2 (M:198.2)	5	0
5128	CYC_HUMAN	Cytochrome c (11.7/10.1)	2.6 (↓)	0.006	97.4 (M:97.4)	3	0
5164	S10A9_HUMAN	Protein S100-A9 (13.2/5.7)	1.7 (↓)	0.009	85.7 (M:85.7)	3	0
5173	S10A8_HUMAN	Protein S100-A8 (10.8/6.6)	1.5 (↓)	0.002	421.3 (M:421.3)	8	0