Fasudil prevents liver fibrosis via activating natural killer cells and suppressing hepatic stellate cells

Table 1 Primers used in this study

Species	Gene	Primer (5' to 3')
Mouse	β-actin	F: ATCATGTTTGAGACCTTCAACA
		R: CATCTCTTGCTCGAAGTCCA
Mouse	MMP2	F: CTCCTGGTATGAGATAGCAAA
		R: TGCACCACCAACTGCTTAGC
Mouse	MMP9	F: TGCATCTTGGCTTTGCAGCTCTTCCTCATGGC
		R: TGGACCTGTGGGTTGTTGACCTCAAACTTGGC
Mouse	α-SMA	F: CTGACAGAGGCACCACTGAA
		R: GAAGGAATAGCCACGCTCAG
Human	α-SMA	F: GATCTCAGTGCAGAGGCTCG
		R: TTTGCTTGTCCAGGTGGTCC
Human	Ki67	F: TCCTCCAGGGATCCAACGA
		R: GGCAGGCGGGAGGTCTT
Human	Bcl-2	F: CTTCCCTCATCCTCCTGCTAC
		R: ACAAACTGGGTAAAGGTGATGG
Human	Bax	F: CTCCTGGTATGAGATAGCAAA
		R: TGCACCACCAACTGCTTAGC
Human	Rho	F: TCCTCCAGGGATCCAACGA
		R: GGCAGGCGGGAGGTCTT
Human	β-actin	F: CACTGTGTTGGCGTACAGGT
		R: TCATCACCATTGGCAATGAG

Note: α-SMA, alpha smooth muscle actin; β-actin, beta actin; Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2; MMP, matrix metalloproteinase; Rho, Ras homology family member

Table 2 Effect of Fasudil on the pathologic grading of hepatic fibrosis mice induce by thioacetamide

Group	Pathologic grading of hepatic fibrosis
	0	I	II	III
Ctrl (n = 5)	15	0	0	0
TAA (n = 5)	0	0	6	7
TAA + Fasudil	0	7	6	2

Results are 15 fields of vision from five mice per group (three fields were chosen from one liver section). TAA: Thioacetamide.