Mucosa repair mechanisms of Tong-Xie-Yao-Fang mediated by CRH-R2 in murine, dextran sulfate sodium-induced colitis

Table 1 Criteria for disease activity index

Score	Weight loss, %	Stool consistency	Bloodstain or gross bleeding
0	None	Normal	Negative
1	1-5	-	-
2	5-10	Loose stool	Positive
3	10-15	-	-
4	> 15	Diarrhea	Gross bleeding

Table 2 Histological score to quantify the degree of colitis

Score	Inflammation	Depth of lesions	Destruction of crypt	Width of lesions, %
0	None	None	None
1	Slight	Mucosa	Basal 1/3 damaged	1-25
2	Moderate	Mucosa and submucosa	Basal 2/3 damaged	26-50
3	Severe	Transmural	Intact epithelium only	51-75
4	-	-	Total crypt and epithelium	76-100

Table 3 Primer sequences and amplification length

Gene	Primer sequences	Amplification length
TNF-α	Forward: 5’-GCCTATGTCTCAGCCTCTTCTC-3’	22
	Reverse: 5’-TGGTGGTTTGCTACGACGTG-3’	20
CXCL-1	Forward: 5’-TCACCTCAAGAACATCCAGAGC-3’	22
	Reverse: 5’-ACTTGGGGACACCTTTTAGCAT-3’	22
IL-6	Forward: 5’-TCTCTGCAAGAGACTTCCATCC-3’	22
	Reverse: 5’-TTCCACGATTTCCCAGAGAACA-3’	22
β-actin	Forward: 5’-AGATCAAGATCATTGCTCCTCC-3’	22
	Reverse: 5’-GGTGTAAAACGCAGCTCAGTAA-3’	22

TNF-α: Tumor necrosis factor-alpha; CXCL-1: Chemokine (C-X-C motif) ligand-1; IL-6: Interleukin-6; β-actin: Beta-actin.