Morin enhances hepatic Nrf2 expression in a liver fibrosis rat model

Table 1 Primer sequences

Name	Primer sequence
Rat Collagen I for	5'-ACTGGTACATCAGCCCAAACCC-3'
Rat Collagen I rev	5'-GGAATCCATCGGTCATGCTCT-3'
Rat Collagen III for	5'-GAGACTCCCCATCATAGATATCGC-3'
Rat Collagen III rev	5'-AGCAAACAGGGCCAATGTCC-3'
Rat α-SMA for	5'-GCTATGCTCTGCCTCATGCC-3'
Rat α-SMA rev	5'-CACGCTCAGCAGTAGTCACGAA-3'
Rat Nrf2 for	5'-ACACAGCATAGCCCATCTCGT-3'
Rat Nrf2 rev	5'-ACCAACCTGGATGAGCGACAC-3'
Rat NQO1 for	5'-CCACGCAGAGAGGACATCATT-3'
Rat NQO1 rev	5'-TTCGACCACCTCCCATCCTT-3'
Rat HO-1 for	5'-CTTCCCGAGCATCGACAAC-3'
Rat HO-1 rev	5'-CTGTCACCCTGTGCTTGACC-3'
Rat Gapdh for	5'-GCTGGTCATCAACGGGAAA-3'
Rat Gapdh rev	5'-CGCCAGTAGACTCCACGACAT-3'

Table 2 Comparison of liver-spleen ratio and liver weight index among different groups

	Control	Morin	CCl4	Morin + CCl4	F	P value
	(n = 5)	(n = 4)	(n = 3)	(n = 4)
LSR	12.27 ± 1.92	12.67 ± 1.60	16.43 ± 1.37ac	15.11 ± 1.99ac	4.668	0.022
LWI%	2.78 ± 0.25	2.80 ± 0.27	4.77 ± 0.47ac	4.17 ± 0.39ace	32.345	< 0.001

^aP < 0.05 vs control group,

^cP < 0.05 vs morin group,

^eP < 0.05 vs CCl₄ group. Liver-spleen ratio (LSR): Liver wet weight/spleen wet weight; liver weight index (LWI): (Liver wet weight/body weight) × l00%.

Table 3 Serum parameters among different groups

	Control	Morin	CCl4	Morin + CCl4	F	P value
	(n = 5)	(n = 4)	(n = 3)	(n = 4)
ALT (IU/L)	101.75 ± 15.46	108.00 ± 48.72	493.33 ± 199.38ac	291.50 ± 111.92ace	11.403	0.001
AST (IU/L)	339.25 ± 72.59	257.80 ± 98.22	1027.67 ± 206.60ac	585.50 ± 131.85ace	26.280	< 0.001
ALP (IU/L)	137.75 ± 29.75	160.80 ± 40.90	377.67 ± 41.07ac	266.50 ± 58.90ace	22.093	< 0.001

^aP < 0.05 vs control group,

^cP < 0.05 vs morin group,

^eP < 0.05 vs CCl₄ group.