Basic Study
Copyright ©The Author(s) 2017.
World J Gastroenterol. Sep 7, 2017; 23(33): 6088-6099
Published online Sep 7, 2017. doi: 10.3748/wjg.v23.i33.6088
Table 1 Serum glucose levels
DateControl group(mmol/L)SEExperimental group (mmol/L)SE
Before streptozotocin injection5.010.105.0300.10
1 wk after streptozotocin injection5.080.1017.360.38
2 wk after streptozotocin injection4.910.1820.720.24
3 wk after streptozotocin injection5.190.0621.580.27
4 wk after streptozotocin injection5.310.1220.080.09
5 wk after streptozotocin injection4.840.3222.261.21
6 wk after streptozotocin injection5.200.1021.451.11
Serum glucose levels in controls and after induction of diabetes by streptozotocin administration (up 1 to 6 wk).
Table 2 The average percentage of neurons immunoreactive to bioactive substances used in this study
Stomach partControl groupExperimental group
AntrumCorpusPylorusAntrumCorpusPylorus
nNOS1nNOS1
Myenteric ganglia37.12 ± 2.8122.28 ± 1.1915.91 ± 0.5841.93 ± 2.3440.74b ± 2.2235.38c ± 1.54
Submucosal ganglia-18.62 ± 1.66--19.79 ± 1.51-
VIP1VIP1
Myenteric ganglia18.38 ± 1.3923.2 ± 0.2323.64 ± 1.5640.74c ± 1.7730.93c ± 0.8631.20a ± 1.10
Submucosal ganglia-43.61 ± 1.06--37.00a ± 1.77-
GAL1GAL1
Myenteric ganglia26.53 ± 1.5217.73 ± 1.1216.32 ± 0.4336.67b ± 1.0216.51 ± 0.9217.99a ± 0.38
Submucosal ganglia-41.42 ± 0.88--40.49 ± 0.63-
1Relative frequency of particular neuronal subclasses is presented as percent (mean ± SE) of all neurons counted within the ganglia stained for Hu C/D proteins. nNOS, VIP and GAL immunoreactive perikarya in various parts of the porcine stomach under physiological conditions (Control group) and during experimentally induced hyperglicemia (Experimental group). Statistically significant data (aP < 0.05,
bP < 0.01, and
cP < 0.001) in particular animal group are marked by superscripted alphabetical lettering. nNOS: Neuronal isoform of nitric oxide synthase; VIP: Vasoactive intestinal peptide; GAL: Galanin; Hu C/D: Pan-neuronal marker.