Copyright
©The Author(s) 2017.
World J Gastroenterol. Apr 14, 2017; 23(14): 2585-2591
Published online Apr 14, 2017. doi: 10.3748/wjg.v23.i14.2585
Published online Apr 14, 2017. doi: 10.3748/wjg.v23.i14.2585
Table 1 Two sets of primers of exon 9 and 20 of PIK3CA gene for polymerase chain reaction
| Exon | Primers | |
| Exon 9 | Forward | 5’CAAAGCAATTTCTACACGAGATCC 3’ |
| Reverse | 5’GTAAAAACATGCTGAGATCAGCCACAT 3’ | |
| Exon 20 | Forward | 5’TGGAATGCCAGAACTACAATCTTT 3’ |
| Reverse | 5’GGTCTTTGCCTGCTGAGAGTT 3’ | |
Table 2 Primers of PIK3CA gene for pyrosequencing
| Exon | Primers | |
| Exon 9 RS1 | Nucleotide dispensation order | 5’ CCATAGAAAATCTTTCTCCT 3’ |
| 5’ ATCGACTACACTGACTGACTGACTGACTGACTGACTG 3’ | ||
| Exon 9 RS2 | Nucleotide dispensation order | 5’ TTCTCCTTGCTTCAGTGATTT 3’ |
| 5’ ATACACATGTCAGTCAGACTAGCTAGCTAGCTAG 3’ | ||
| Exon 9 RS3 | Nucleotide dispensation order | 5’ TAGAAAATCTTTCTCCTGCT 3’ |
| 5’ ATAGCACTGACTGACTGACTACTGACTGACTGACTG 3’ | ||
| Exon 20 RS | Nucleotide dispensation order | 5’TGGAATGCCAGAACTACAATCTTT 3’ |
| 5’GGTCTTTGCCTGCTGAGAGTT 3’ | ||
Table 3 PIK3CA mutations and clinicopathological characteristics in Northwest Chinese esophageal squamous cell carcinoma patients n (%)
| Clinical, epidemiologic, or pathologic feature | Total, n | PIK3CA | P value | |
| Mutant | Wild-type | |||
| All cases | 210 | 48 | 162 | |
| Sex | 0.4756 | |||
| Male | 137 (65.3) | 34 (70.8) | 123 (75.9) | |
| Female | 73 (34.7) | 14 (29.2) | 39 (24.1) | |
| Tobacco use | 0.2684 | |||
| Yes | 149 (71.0) | 31 (64.6) | 118 (72.9) | |
| No | 61 (29.0) | 17 (35.4) | 44 (28.1) | |
| Alcohol use | 0.3778 | |||
| Yes | 175 (83.3) | 38 (79.2) | 137 (84.6) | |
| No | 35 (16.7) | 10 (20.8) | 25 (15.4) | |
| Preoperative treatment | 0.8467 | |||
| Yes | 28 (13.3) | 6 (12.5) | 22 (13.6) | |
| No | 182 (86.7) | 42 (87.5) | 140 (86.4) | |
| Tumor location | 0.9651 | |||
| Upper thoracic | 20 (9.5) | 5 (10.4) | 15 (9.2) | |
| Middle thoracic | 109 (51.9) | 25 (52.1) | 84 (51.9) | |
| Lower thoracic | 81 (38.6) | 18 (37.5) | 63 (38.9) | |
| Stage | 0.1641 | |||
| IA | 16 (7.6) | 3 (6.3) | 13 (8.0) | |
| IB | 20 (9.5) | 5 (10.4) | 15 (9.3) | |
| IIA | 28 (13.3) | 11 (22.9) | 17 (10.5) | |
| IIB | 44 (21.0) | 10 (20.8) | 34 (21.0) | |
| IIIA | 49 (23.3) | 12 (25.0) | 37 (22.8) | |
| IIIB | 23 (11.0) | 1 (2.1) | 22 (13.6) | |
| IIIC | 30 (14.3) | 6 (12.5) | 24 (14.8) | |
| Lymph node metastasis | 0.2663 | |||
| Yes | 121 (57.6) | 31 (64.6) | 90 (55.6) | |
| No | 89 (42.4) | 17 (35.4) | 72 (44.4) | |
| Local recurrence | 0.7368 | |||
| Yes | 43 (20.5) | 9 (18.8) | 34 (21.0) | |
| No | 167 (79.5) | 39 (81.2) | 128 (79.0) | |
| Prognosis | 0.0885 | |||
| Dead | 88 (41.9) | 15 (31.3) | 73 (45.1) | |
| Survived | 122 (58.1) | 33 (68.7) | 89 (54.9) | |
- Citation: Liu SY, Chen W, Chughtai EA, Qiao Z, Jiang JT, Li SM, Zhang W, Zhang J. PIK3CA gene mutations in Northwest Chinese esophageal squamous cell carcinoma. World J Gastroenterol 2017; 23(14): 2585-2591
- URL: https://www.wjgnet.com/1007-9327/full/v23/i14/2585.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i14.2585