Xiaotan Sanjie decoction inhibits interleukin-8-induced metastatic potency in gastric cancer

Table 1 Primer sequences used for quantitative reverse transcription-polymerase chain reaction

mRNA	Sense primer sequence	bp
hGAPDH-F	5’-GGGTGTGAACCATGAGAAGTATG-3’	145
hGAPDH-R	5’-GATGGCATGGACTGTGGTCAT-3’
CD44-F	5’-ATGGACAAGTTTTGGTGGCA-3’	230
CD44-R	5’-CAGGTCTCAAATCCGATGCTC-3’

F: Forward; R: Reverse.

Table 2 Effects of Xiaotan Sanjie decoction and interleukin-8 on adhesion and invasion of SGC-7901 cells

Group	Adhesion(OD value)bd	Invasion(Invasive cell number)bdf
Blank group	0.186 ± 0.012	785.00 ± 48.13
IL-8 group	0.213 ± 0.003	1103.33 ± 134.52
XTSJ group	0.136 ± 0.002	394.25 ± 62.20
XTSJ + IL-8 group	0.147 ± 0.001	363.25 ± 31.63

^bP < 0.01 vs IL-8;

^dP < 0.01 vs XTSJ decoction;

^fP < 0.01 vs IL-8 × XTSJ decoction. XTSJ: Xiaotan Sanjie; IL-8: Interleukin-8.

Table 3 Effects of Xiaotan Sanjie decoction and interleukin-8 on CD44 protein and mRNA expression

Group	CD44 proteinad	CD44 mRNAbce
Blank group	1.00 ± 0.00	1.00 ± 0.06
IL-8 group	1.05 ± 0.04	1.52 ± 0.06
XTSJ group	0.60 ± 0.08	1.30 ± 0.12
XTSJ + IL-8 group	0.71 ± 0.04	1.37 ± 0.18

^aP < 0.05,

^bP < 0.01 vs IL-8;

^cP < 0.05,

^dP < 0.01 vs XTSJ decoction;

^eP < 0.05, vs IL-8 × XTSJ decoction. XTSJ: Xiaotan Sanjie; IL-8: Interleukin-8.